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ABA signalling, grafting, irrigation scheduling, partial rootzone drying, tomato, water use.

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ABA signalling, grafting, irrigation scheduling, partial rootzone drying, tomato, water use. / Belimov, A. A.; Dodd, Ian C.; Safronova, V. I. et al.
In: Journal of Experimental Botany, Vol. 58, No. 6, 04.2007, p. 1485-1495.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Belimov AA, Dodd IC, Safronova VI, Hontzeas N, Davies WJ. ABA signalling, grafting, irrigation scheduling, partial rootzone drying, tomato, water use. Journal of Experimental Botany. 2007 Apr;58(6):1485-1495. doi: 10.1093/jxb/erm010

Author

Belimov, A. A. ; Dodd, Ian C. ; Safronova, V. I. et al. / ABA signalling, grafting, irrigation scheduling, partial rootzone drying, tomato, water use. In: Journal of Experimental Botany. 2007 ; Vol. 58, No. 6. pp. 1485-1495.

Bibtex

@article{ca849a607d5745d5858629c2a0348be1,
title = "ABA signalling, grafting, irrigation scheduling, partial rootzone drying, tomato, water use.",
abstract = "The role of bacterial 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity in the interaction between tomato (Lycopersicon esculentum=Solanum lycopersicum) and Pseudomonas brassicacearum was studied in different strains. The phytopathogenic strain 520-1 possesses ACC deaminase activity, an important trait of plant growth-promoting rhizobacteria (PGPR) that stimulates root growth. The ACC-utilizing PGPR strain Am3 increased in vitro root elongation and root biomass of soil-grown tomato cv. Ailsa Craig at low bacterial concentrations (106 cells ml–1 in vitro and 106 cells g–1 soil) but had negative effects on in vitro root elongation at higher bacterial concentrations. A mutant strain of Am3 (designated T8-1) that was engineered to be ACC deaminase deficient failed to promote tomato root growth in vitro and in soil. Although strains T8-1 and 520-1 inhibited root growth in vitro at higher bacterial concentrations (>106 cells ml–1), they did not cause disease symptoms in vitro after seed inoculation, or in soil supplemented with bacteria. All the P. brassicacearum strains studied caused pith necrosis when stems or fruits were inoculated with a bacterial suspension, as did the causal organism of this disease (P. corrugata 176), but the non-pathogenic strain Pseudomonas sp. Dp2 did not. Strains Am3 and T8-1 were marked with antibiotic resistance and fluorescence to show that bacteria introduced to the nutrient solution or on seeds in vitro, or in soil were capable of colonizing the root surface, but were not detected inside root tissues. Both strains showed similar colonization ability either on root surfaces or in wounded stems. The results suggest that bacterial ACC deaminase of P. brassicacearum Am3 can promote growth in tomato by masking the phytopathogenic properties of this bacterium.",
keywords = "ACC deaminase, colonization, ethylene, GFP, PGPR, phytopathogen, plant–bacteria interactions, Pseudomonas, rhizosphere, tomato",
author = "Belimov, {A. A.} and Dodd, {Ian C.} and Safronova, {V. I.} and N. Hontzeas and Davies, {William J.}",
note = "Copyright {\textcopyright} 2007 Oxford University Press",
year = "2007",
month = apr,
doi = "10.1093/jxb/erm010",
language = "English",
volume = "58",
pages = "1485--1495",
journal = "Journal of Experimental Botany",
issn = "1460-2431",
publisher = "OXFORD UNIV PRESS",
number = "6",

}

RIS

TY - JOUR

T1 - ABA signalling, grafting, irrigation scheduling, partial rootzone drying, tomato, water use.

AU - Belimov, A. A.

AU - Dodd, Ian C.

AU - Safronova, V. I.

AU - Hontzeas, N.

AU - Davies, William J.

N1 - Copyright © 2007 Oxford University Press

PY - 2007/4

Y1 - 2007/4

N2 - The role of bacterial 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity in the interaction between tomato (Lycopersicon esculentum=Solanum lycopersicum) and Pseudomonas brassicacearum was studied in different strains. The phytopathogenic strain 520-1 possesses ACC deaminase activity, an important trait of plant growth-promoting rhizobacteria (PGPR) that stimulates root growth. The ACC-utilizing PGPR strain Am3 increased in vitro root elongation and root biomass of soil-grown tomato cv. Ailsa Craig at low bacterial concentrations (106 cells ml–1 in vitro and 106 cells g–1 soil) but had negative effects on in vitro root elongation at higher bacterial concentrations. A mutant strain of Am3 (designated T8-1) that was engineered to be ACC deaminase deficient failed to promote tomato root growth in vitro and in soil. Although strains T8-1 and 520-1 inhibited root growth in vitro at higher bacterial concentrations (>106 cells ml–1), they did not cause disease symptoms in vitro after seed inoculation, or in soil supplemented with bacteria. All the P. brassicacearum strains studied caused pith necrosis when stems or fruits were inoculated with a bacterial suspension, as did the causal organism of this disease (P. corrugata 176), but the non-pathogenic strain Pseudomonas sp. Dp2 did not. Strains Am3 and T8-1 were marked with antibiotic resistance and fluorescence to show that bacteria introduced to the nutrient solution or on seeds in vitro, or in soil were capable of colonizing the root surface, but were not detected inside root tissues. Both strains showed similar colonization ability either on root surfaces or in wounded stems. The results suggest that bacterial ACC deaminase of P. brassicacearum Am3 can promote growth in tomato by masking the phytopathogenic properties of this bacterium.

AB - The role of bacterial 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity in the interaction between tomato (Lycopersicon esculentum=Solanum lycopersicum) and Pseudomonas brassicacearum was studied in different strains. The phytopathogenic strain 520-1 possesses ACC deaminase activity, an important trait of plant growth-promoting rhizobacteria (PGPR) that stimulates root growth. The ACC-utilizing PGPR strain Am3 increased in vitro root elongation and root biomass of soil-grown tomato cv. Ailsa Craig at low bacterial concentrations (106 cells ml–1 in vitro and 106 cells g–1 soil) but had negative effects on in vitro root elongation at higher bacterial concentrations. A mutant strain of Am3 (designated T8-1) that was engineered to be ACC deaminase deficient failed to promote tomato root growth in vitro and in soil. Although strains T8-1 and 520-1 inhibited root growth in vitro at higher bacterial concentrations (>106 cells ml–1), they did not cause disease symptoms in vitro after seed inoculation, or in soil supplemented with bacteria. All the P. brassicacearum strains studied caused pith necrosis when stems or fruits were inoculated with a bacterial suspension, as did the causal organism of this disease (P. corrugata 176), but the non-pathogenic strain Pseudomonas sp. Dp2 did not. Strains Am3 and T8-1 were marked with antibiotic resistance and fluorescence to show that bacteria introduced to the nutrient solution or on seeds in vitro, or in soil were capable of colonizing the root surface, but were not detected inside root tissues. Both strains showed similar colonization ability either on root surfaces or in wounded stems. The results suggest that bacterial ACC deaminase of P. brassicacearum Am3 can promote growth in tomato by masking the phytopathogenic properties of this bacterium.

KW - ACC deaminase

KW - colonization

KW - ethylene

KW - GFP

KW - PGPR

KW - phytopathogen

KW - plant–bacteria interactions

KW - Pseudomonas

KW - rhizosphere

KW - tomato

U2 - 10.1093/jxb/erm010

DO - 10.1093/jxb/erm010

M3 - Journal article

VL - 58

SP - 1485

EP - 1495

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 1460-2431

IS - 6

ER -