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Abscisic acid-induced elevation of guard cell cytosolic Ca2+ precedes stomatal closure.

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Abscisic acid-induced elevation of guard cell cytosolic Ca2+ precedes stomatal closure. / McAinsh, Martin R.; Brownlee, C.; Hetherington, Alistair M.

In: Nature, Vol. 343, No. 6254, 11.01.1990, p. 186-188.

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McAinsh, Martin R. ; Brownlee, C. ; Hetherington, Alistair M. / Abscisic acid-induced elevation of guard cell cytosolic Ca2+ precedes stomatal closure. In: Nature. 1990 ; Vol. 343, No. 6254. pp. 186-188.

Bibtex

@article{36cb6e8ce5e843eeae9b0dea3cbb78e7,
title = "Abscisic acid-induced elevation of guard cell cytosolic Ca2+ precedes stomatal closure.",
abstract = "STOMATTA allow the diffusion of CO2 into the leaf for photosynthesis and the diffusion of H2O out of the leaf during transpiration1,2. This gaseous exchange is regulated by pairs of guard cells that surround each stomatal pore. During water stress the loss of water through transpiration is reduced in response to abscisic acid3, a naturally occurring plant growth regulator which is also present in certain mammals4, algae5 and fungi6, by the promotion of stomatal closure and inhibition of opening7. This involves alterations to guard cell turgor, causing the cells to shrink and thereby reducing the size of the stomatal pore. These changes are driven by cation and anion effluxes8. It has been proposed that an abscisic acid-dependent increase in the concentration of guard cell cytosolic free calcium triggers the intracellular machinery responsible for stomatal closure9(for a review, see ref. 10), but attempts to test this hypothesis by measuring [45Ca] fluxes have produced equivocal results11. Using the fluorescent calcium indicator fura-2, we report that abscisic acid induces a rapid increase in guard cell cytosolic free Ca2+ in Commelina communisL., and that this increase precedes stomatal closure. These results strongly support the suggestion that Ca2+ is an intracellular second messenger in this response.",
keywords = "stomata, cytosolic calcium, Ca2+, fura-2, second messenger",
author = "McAinsh, {Martin R.} and C. Brownlee and Hetherington, {Alistair M.}",
year = "1990",
month = jan,
day = "11",
doi = "10.1038/343186a0",
language = "English",
volume = "343",
pages = "186--188",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6254",

}

RIS

TY - JOUR

T1 - Abscisic acid-induced elevation of guard cell cytosolic Ca2+ precedes stomatal closure.

AU - McAinsh, Martin R.

AU - Brownlee, C.

AU - Hetherington, Alistair M.

PY - 1990/1/11

Y1 - 1990/1/11

N2 - STOMATTA allow the diffusion of CO2 into the leaf for photosynthesis and the diffusion of H2O out of the leaf during transpiration1,2. This gaseous exchange is regulated by pairs of guard cells that surround each stomatal pore. During water stress the loss of water through transpiration is reduced in response to abscisic acid3, a naturally occurring plant growth regulator which is also present in certain mammals4, algae5 and fungi6, by the promotion of stomatal closure and inhibition of opening7. This involves alterations to guard cell turgor, causing the cells to shrink and thereby reducing the size of the stomatal pore. These changes are driven by cation and anion effluxes8. It has been proposed that an abscisic acid-dependent increase in the concentration of guard cell cytosolic free calcium triggers the intracellular machinery responsible for stomatal closure9(for a review, see ref. 10), but attempts to test this hypothesis by measuring [45Ca] fluxes have produced equivocal results11. Using the fluorescent calcium indicator fura-2, we report that abscisic acid induces a rapid increase in guard cell cytosolic free Ca2+ in Commelina communisL., and that this increase precedes stomatal closure. These results strongly support the suggestion that Ca2+ is an intracellular second messenger in this response.

AB - STOMATTA allow the diffusion of CO2 into the leaf for photosynthesis and the diffusion of H2O out of the leaf during transpiration1,2. This gaseous exchange is regulated by pairs of guard cells that surround each stomatal pore. During water stress the loss of water through transpiration is reduced in response to abscisic acid3, a naturally occurring plant growth regulator which is also present in certain mammals4, algae5 and fungi6, by the promotion of stomatal closure and inhibition of opening7. This involves alterations to guard cell turgor, causing the cells to shrink and thereby reducing the size of the stomatal pore. These changes are driven by cation and anion effluxes8. It has been proposed that an abscisic acid-dependent increase in the concentration of guard cell cytosolic free calcium triggers the intracellular machinery responsible for stomatal closure9(for a review, see ref. 10), but attempts to test this hypothesis by measuring [45Ca] fluxes have produced equivocal results11. Using the fluorescent calcium indicator fura-2, we report that abscisic acid induces a rapid increase in guard cell cytosolic free Ca2+ in Commelina communisL., and that this increase precedes stomatal closure. These results strongly support the suggestion that Ca2+ is an intracellular second messenger in this response.

KW - stomata

KW - cytosolic calcium

KW - Ca2+

KW - fura-2

KW - second messenger

U2 - 10.1038/343186a0

DO - 10.1038/343186a0

M3 - Journal article

VL - 343

SP - 186

EP - 188

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6254

ER -