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Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose.

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Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose. / Leckie, Callum P.; McAinsh, Martin R.; Allen, Gethyn J. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 26, 22.12.1998, p. 15837-15842.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Leckie, CP, McAinsh, MR, Allen, GJ, Sanders, D & Hetherington, AM 1998, 'Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose.', Proceedings of the National Academy of Sciences of the United States of America, vol. 95, no. 26, pp. 15837-15842. <http://www.pnas.org/content/95/26/15837.full>

APA

Leckie, C. P., McAinsh, M. R., Allen, G. J., Sanders, D., & Hetherington, A. M. (1998). Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose. Proceedings of the National Academy of Sciences of the United States of America, 95(26), 15837-15842. http://www.pnas.org/content/95/26/15837.full

Vancouver

Leckie CP, McAinsh MR, Allen GJ, Sanders D, Hetherington AM. Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose. Proceedings of the National Academy of Sciences of the United States of America. 1998 Dec 22;95(26):15837-15842.

Author

Leckie, Callum P. ; McAinsh, Martin R. ; Allen, Gethyn J. et al. / Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 26. pp. 15837-15842.

Bibtex

@article{fc3bc06944954d33aedacfcfcd21ff24,
title = "Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose.",
abstract = "Abscisic acid (ABA) is a plant hormone involved in the response of plants to reduced water availability. Reduction of guard cell turgor by ABA diminishes the aperture of the stomatal pore and thereby contributes to the ability of the plant to conserve water during periods of drought. Previous work has demonstrated that cytosolic Ca2+ is involved in the signal transduction pathway that mediates the reduction in guard cell turgor elicited by ABA. Here we report that ABA uses a Ca2+-mobilization pathway that involves cyclic adenosine 5′-diphosphoribose (cADPR). Microinjection of cADPR into guard cells caused reductions in turgor that were preceded by increases in the concentration of free Ca2+ in the cytosol. Patch clamp measurements of isolated guard cell vacuoles revealed the presence of a cADPR-elicited Ca2+-selective current that was inhibited at cytosolic Ca2+ ≥ 600 nM. Furthermore, microinjection of the cADPR antagonist 8-NH2-cADPR caused a reduction in the rate of turgor loss in response to ABA in 54% of cells tested, and nicotinamide, an antagonist of cADPR production, elicited a dose-dependent block of ABA-induced stomatal closure. Our data provide definitive evidence for a physiological role for cADPR and illustrate one mechanism of stimulus-specific Ca2+ mobilization in higher plants. Taken together with other recent data [Wu, Y., Kuzma, J., Marechal, E., Graeff, R., Lee, H. C., Foster, R. & Chua, N.-H. (1997) Science 278, 2126–2130], these results establish cADPR as a key player in ABA signal transduction pathways in plants.",
keywords = "cADPR, guard cell, stomata, Ca2+, ABA, cytosolic calcium",
author = "Leckie, {Callum P.} and McAinsh, {Martin R.} and Allen, {Gethyn J.} and Dale Sanders and Hetherington, {Alistair M.}",
year = "1998",
month = dec,
day = "22",
language = "English",
volume = "95",
pages = "15837--15842",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "26",

}

RIS

TY - JOUR

T1 - Abscisic acid-mediates stomatal closure mediated by cyclic ADP-ribose.

AU - Leckie, Callum P.

AU - McAinsh, Martin R.

AU - Allen, Gethyn J.

AU - Sanders, Dale

AU - Hetherington, Alistair M.

PY - 1998/12/22

Y1 - 1998/12/22

N2 - Abscisic acid (ABA) is a plant hormone involved in the response of plants to reduced water availability. Reduction of guard cell turgor by ABA diminishes the aperture of the stomatal pore and thereby contributes to the ability of the plant to conserve water during periods of drought. Previous work has demonstrated that cytosolic Ca2+ is involved in the signal transduction pathway that mediates the reduction in guard cell turgor elicited by ABA. Here we report that ABA uses a Ca2+-mobilization pathway that involves cyclic adenosine 5′-diphosphoribose (cADPR). Microinjection of cADPR into guard cells caused reductions in turgor that were preceded by increases in the concentration of free Ca2+ in the cytosol. Patch clamp measurements of isolated guard cell vacuoles revealed the presence of a cADPR-elicited Ca2+-selective current that was inhibited at cytosolic Ca2+ ≥ 600 nM. Furthermore, microinjection of the cADPR antagonist 8-NH2-cADPR caused a reduction in the rate of turgor loss in response to ABA in 54% of cells tested, and nicotinamide, an antagonist of cADPR production, elicited a dose-dependent block of ABA-induced stomatal closure. Our data provide definitive evidence for a physiological role for cADPR and illustrate one mechanism of stimulus-specific Ca2+ mobilization in higher plants. Taken together with other recent data [Wu, Y., Kuzma, J., Marechal, E., Graeff, R., Lee, H. C., Foster, R. & Chua, N.-H. (1997) Science 278, 2126–2130], these results establish cADPR as a key player in ABA signal transduction pathways in plants.

AB - Abscisic acid (ABA) is a plant hormone involved in the response of plants to reduced water availability. Reduction of guard cell turgor by ABA diminishes the aperture of the stomatal pore and thereby contributes to the ability of the plant to conserve water during periods of drought. Previous work has demonstrated that cytosolic Ca2+ is involved in the signal transduction pathway that mediates the reduction in guard cell turgor elicited by ABA. Here we report that ABA uses a Ca2+-mobilization pathway that involves cyclic adenosine 5′-diphosphoribose (cADPR). Microinjection of cADPR into guard cells caused reductions in turgor that were preceded by increases in the concentration of free Ca2+ in the cytosol. Patch clamp measurements of isolated guard cell vacuoles revealed the presence of a cADPR-elicited Ca2+-selective current that was inhibited at cytosolic Ca2+ ≥ 600 nM. Furthermore, microinjection of the cADPR antagonist 8-NH2-cADPR caused a reduction in the rate of turgor loss in response to ABA in 54% of cells tested, and nicotinamide, an antagonist of cADPR production, elicited a dose-dependent block of ABA-induced stomatal closure. Our data provide definitive evidence for a physiological role for cADPR and illustrate one mechanism of stimulus-specific Ca2+ mobilization in higher plants. Taken together with other recent data [Wu, Y., Kuzma, J., Marechal, E., Graeff, R., Lee, H. C., Foster, R. & Chua, N.-H. (1997) Science 278, 2126–2130], these results establish cADPR as a key player in ABA signal transduction pathways in plants.

KW - cADPR

KW - guard cell

KW - stomata

KW - Ca2+

KW - ABA

KW - cytosolic calcium

M3 - Journal article

VL - 95

SP - 15837

EP - 15842

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 26

ER -