Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Age-dependent transformation frequency in elite wheat varieties
AU - Pastori, G. M.
AU - Wilkinson, M. D.
AU - Steele, S. H.
AU - Sparks, C. A.
AU - Jones, H. D.
AU - Parry, M. A J
PY - 2001/7/20
Y1 - 2001/7/20
N2 - Wheat is a major world crop and as such is a primary target for improvement of agronomic characteristics via genetic engineering. Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varieties is not always successful due to variability in regeneration and transformation frequencies between varieties. In this work, two elite wheat varieties with a relatively high embryogenic capacity were transformed by particle bombardment. A strong correlation between transformation frequency and the age of wheat donor plants was observed in both varieties. The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally-controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypicaily normal, fertile, transgenic plants.
AB - Wheat is a major world crop and as such is a primary target for improvement of agronomic characteristics via genetic engineering. Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varieties is not always successful due to variability in regeneration and transformation frequencies between varieties. In this work, two elite wheat varieties with a relatively high embryogenic capacity were transformed by particle bombardment. A strong correlation between transformation frequency and the age of wheat donor plants was observed in both varieties. The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally-controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypicaily normal, fertile, transgenic plants.
KW - Bar gene
KW - Elite wheat varieties
KW - Transformation frequency
KW - uidA gene
KW - Wheat transformation
UR - http://www.scopus.com/inward/record.url?scp=0034936403&partnerID=8YFLogxK
U2 - 10.1093/jexbot/52.357.857
DO - 10.1093/jexbot/52.357.857
M3 - Journal article
C2 - 11413223
AN - SCOPUS:0034936403
VL - 52
SP - 857
EP - 863
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 357
ER -