Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea

Biomedical and Life Sciences

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Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea. / Baronio, Diego; Martinez, Denis; Fiori, Cintia Zappe et al.
In: Respiratory physiology & neurobiology, Vol. 185, No. 2, 15.01.2013, p. 217-221.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Baronio, D, Martinez, D, Fiori, CZ, Bambini-Junior, V, Forgiarini, LF, Pase da Rosa, D, Kim, LJ & Cerski, MR 2013, 'Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea', Respiratory physiology & neurobiology, vol. 185, no. 2, pp. 217-221. https://doi.org/10.1016/j.resp.2012.10.012

APA

Baronio, D., Martinez, D., Fiori, C. Z., Bambini-Junior, V., Forgiarini, L. F., Pase da Rosa, D., Kim, L. J., & Cerski, M. R. (2013). Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea. Respiratory physiology & neurobiology, 185(2), 217-221. https://doi.org/10.1016/j.resp.2012.10.012

Vancouver

Baronio D, Martinez D, Fiori CZ, Bambini-Junior V, Forgiarini LF, Pase da Rosa D et al. Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea. Respiratory physiology & neurobiology. 2013 Jan 15;185(2):217-221. Epub 2012 Oct 30. doi: 10.1016/j.resp.2012.10.012

Author

Baronio, Diego ; Martinez, Denis ; Fiori, Cintia Zappe et al. / Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea. In: Respiratory physiology & neurobiology. 2013 ; Vol. 185, No. 2. pp. 217-221.

Bibtex

@article{22175c40248747e48d0c4742da182d3e,

title = "Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea",

abstract = "Rostral fluid displacement has been proposed as a pathophysiologic mechanism of both central and obstructive sleep apnea. Aquaporins are membrane proteins that regulate water transport across the cell membrane and are involved in brain edema formation and resolution. The present study investigated the effect of intermittent hypoxia (IH), a model of sleep apnea, on brain aquaporins. Mice were exposed to intermittent hypoxia to a nadir of 7% oxygen fraction. Brain water content, Aquaporin-1 and Aquaporin-3 were measured in the cerebellum and hippocampus. Hematoxylin-eosin and immunohistochemistry stainings were performed to evaluate cell damage. Compared to the sham group, the hypoxia group presented higher brain water content, lower levels of Aquaporin-1 and similar levels of Aquaporin-3. Immunoreactivity to GFAP and S100B was stronger in the hypoxia group in areas of extensive gliosis, compatible with cytotoxic edema. These findings, although preliminary, indicate an effect of IH on aquaporins levels. Further investigation about the relevance of these data on the pathophysiology of OSA is warranted.",

keywords = "Animals, Aquaporins/metabolism, Brain/metabolism, Disease Models, Animal, Glial Fibrillary Acidic Protein/metabolism, Hypoxia/complications, Male, Mice, Mice, Inbred C57BL, Nerve Growth Factors/metabolism, Organ Size, S100 Calcium Binding Protein beta Subunit, S100 Proteins/metabolism, Sleep Apnea Syndromes/etiology, Statistics, Nonparametric, Water/metabolism",

author = "Diego Baronio and Denis Martinez and Fiori, {Cintia Zappe} and Victorio Bambini-Junior and Forgiarini, {Luiz Felipe} and {Pase da Rosa}, Darlan and Kim, {Lenise Jihe} and Cerski, {Marcelle Reesink}",

year = "2013",

month = jan,

day = "15",

doi = "10.1016/j.resp.2012.10.012",

language = "English",

volume = "185",

pages = "217--221",

journal = "Respiratory physiology & neurobiology",

issn = "1569-9048",

publisher = "Elsevier",

number = "2",

}

RIS

TY - JOUR

T1 - Altered aquaporins in the brains of mice submitted to intermittent hypoxia model of sleep apnea

AU - Baronio, Diego

AU - Martinez, Denis

AU - Fiori, Cintia Zappe

AU - Bambini-Junior, Victorio

AU - Forgiarini, Luiz Felipe

AU - Pase da Rosa, Darlan

AU - Kim, Lenise Jihe

AU - Cerski, Marcelle Reesink

PY - 2013/1/15

Y1 - 2013/1/15

N2 - Rostral fluid displacement has been proposed as a pathophysiologic mechanism of both central and obstructive sleep apnea. Aquaporins are membrane proteins that regulate water transport across the cell membrane and are involved in brain edema formation and resolution. The present study investigated the effect of intermittent hypoxia (IH), a model of sleep apnea, on brain aquaporins. Mice were exposed to intermittent hypoxia to a nadir of 7% oxygen fraction. Brain water content, Aquaporin-1 and Aquaporin-3 were measured in the cerebellum and hippocampus. Hematoxylin-eosin and immunohistochemistry stainings were performed to evaluate cell damage. Compared to the sham group, the hypoxia group presented higher brain water content, lower levels of Aquaporin-1 and similar levels of Aquaporin-3. Immunoreactivity to GFAP and S100B was stronger in the hypoxia group in areas of extensive gliosis, compatible with cytotoxic edema. These findings, although preliminary, indicate an effect of IH on aquaporins levels. Further investigation about the relevance of these data on the pathophysiology of OSA is warranted.

AB - Rostral fluid displacement has been proposed as a pathophysiologic mechanism of both central and obstructive sleep apnea. Aquaporins are membrane proteins that regulate water transport across the cell membrane and are involved in brain edema formation and resolution. The present study investigated the effect of intermittent hypoxia (IH), a model of sleep apnea, on brain aquaporins. Mice were exposed to intermittent hypoxia to a nadir of 7% oxygen fraction. Brain water content, Aquaporin-1 and Aquaporin-3 were measured in the cerebellum and hippocampus. Hematoxylin-eosin and immunohistochemistry stainings were performed to evaluate cell damage. Compared to the sham group, the hypoxia group presented higher brain water content, lower levels of Aquaporin-1 and similar levels of Aquaporin-3. Immunoreactivity to GFAP and S100B was stronger in the hypoxia group in areas of extensive gliosis, compatible with cytotoxic edema. These findings, although preliminary, indicate an effect of IH on aquaporins levels. Further investigation about the relevance of these data on the pathophysiology of OSA is warranted.

KW - Animals

KW - Aquaporins/metabolism

KW - Brain/metabolism

KW - Disease Models, Animal

KW - Glial Fibrillary Acidic Protein/metabolism

KW - Hypoxia/complications

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Nerve Growth Factors/metabolism

KW - Organ Size

KW - S100 Calcium Binding Protein beta Subunit

KW - S100 Proteins/metabolism

KW - Sleep Apnea Syndromes/etiology

KW - Statistics, Nonparametric

KW - Water/metabolism

U2 - 10.1016/j.resp.2012.10.012

DO - 10.1016/j.resp.2012.10.012

M3 - Journal article

C2 - 23123204

VL - 185

SP - 217

EP - 221

JO - Respiratory physiology & neurobiology

JF - Respiratory physiology & neurobiology

SN - 1569-9048

IS - 2

ER -

Research

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