TY - JOUR

T1 - Amino acids loaded chitosan/collagen based new membranes stimulate angiogenesis in chorioallantoic membrane assay

AU - Aleem, A.R.

AU - Shahzadi, L.

AU - Tehseen, S.

AU - Alvi, F.

AU - Chaudhry, A.A.

AU - Rehman, I.U.

AU - Yar, M.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Chitosan/collagen-based hydrogels were studied for their promising role in skin tissue engineering applications due to their unique biocompatibility and biodegradation properties. Amino acids are not only the mean of protein building units but also support endothelial cells proliferation and trigger angiogenesis during wound healing. The purpose of this study was to prepare amino acid based pro-angiogenic materials. Three structurally closed amino acids (AA) (arginine, alanine and phenylalanine) were loaded into chitosan/collagen hydrogels (ACC hydrogels) to study their effect on angiogenesis. In this study the ACC hydrogels were prepared through freeze drying procedure and their angiogenic potential was studied by chorioallantoic membrane assay (CAM assay). FTIR analysis was performed to confirm that there was no chemical change took place in polymeric materials during synthesis procedures. Results revealed that, arginine-loaded hydrogels were the most porous, with more interconnected pores and also the maximum growth of blood vessels were found around and inside the arginine loaded scaffold. The qualitative analysis for blood vessels showed the significant difference between control, chitosan/collagen alanine loaded hydrogel (CH-Ala), chitosan/collagen phenylalanine loaded hydrogel (CH-Phe) and chitosan/collagen arginine loaded hydrogel (CH-Arg) materials. Among these studied materials the CH-Arg was found more capable for angiogenesis.

AB - Chitosan/collagen-based hydrogels were studied for their promising role in skin tissue engineering applications due to their unique biocompatibility and biodegradation properties. Amino acids are not only the mean of protein building units but also support endothelial cells proliferation and trigger angiogenesis during wound healing. The purpose of this study was to prepare amino acid based pro-angiogenic materials. Three structurally closed amino acids (AA) (arginine, alanine and phenylalanine) were loaded into chitosan/collagen hydrogels (ACC hydrogels) to study their effect on angiogenesis. In this study the ACC hydrogels were prepared through freeze drying procedure and their angiogenic potential was studied by chorioallantoic membrane assay (CAM assay). FTIR analysis was performed to confirm that there was no chemical change took place in polymeric materials during synthesis procedures. Results revealed that, arginine-loaded hydrogels were the most porous, with more interconnected pores and also the maximum growth of blood vessels were found around and inside the arginine loaded scaffold. The qualitative analysis for blood vessels showed the significant difference between control, chitosan/collagen alanine loaded hydrogel (CH-Ala), chitosan/collagen phenylalanine loaded hydrogel (CH-Phe) and chitosan/collagen arginine loaded hydrogel (CH-Arg) materials. Among these studied materials the CH-Arg was found more capable for angiogenesis.

KW - Chitosan

KW - Collagen

KW - Phenylalanine

KW - Arginine

KW - Alanine

KW - Angiogenesis

U2 - 10.1016/j.ijbiomac.2019.08.095

DO - 10.1016/j.ijbiomac.2019.08.095

M3 - Journal article

VL - 140

SP - 401

EP - 406

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -