Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment

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Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment. / Hiorns, W. D.; Hastings, R. C.; Head, I. M. et al.
In: Microbiology, Vol. 141, No. 11, 01.11.1995, p. 2793-2800.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Hiorns, WD, Hastings, RC, Head, IM, McCarthy, AJ, Saunders, JR, Pickup, RW & Hall, GH 1995, 'Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment', Microbiology, vol. 141, no. 11, pp. 2793-2800. https://doi.org/10.1099/13500872-141-11-2793

APA

Hiorns, W. D., Hastings, R. C., Head, I. M., McCarthy, A. J., Saunders, J. R., Pickup, R. W., & Hall, G. H. (1995). Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment. Microbiology, 141(11), 2793-2800. https://doi.org/10.1099/13500872-141-11-2793

Vancouver

Hiorns WD, Hastings RC, Head IM, McCarthy AJ, Saunders JR, Pickup RW et al. Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment. Microbiology. 1995 Nov 1;141(11):2793-2800. doi: 10.1099/13500872-141-11-2793

Author

Hiorns, W. D. ; Hastings, R. C. ; Head, I. M. et al. / Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment. In: Microbiology. 1995 ; Vol. 141, No. 11. pp. 2793-2800.

Bibtex

@article{2ca6d4013d6441ebabf7b2a18b7c9ef9,

title = "Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment",

abstract = "Oligonucleotide sequences selected from the 16S rRNA genes of various species of ammonia-oxidizing bacteria were evaluated as specific PCR amplification primers and probes. The specificities of primer pairs for eubacterial, Nitrosospira and Nitrosomonas rRNA genes were established with sequence databases, and the primer pairs were used to amplify DNA from laboratory cultures and environmental samples. Eubacterial rRNA genes amplified from samples of soil and activated sludge hybridized with an oligonucleotide probe specific for Nitrosospira spp., but not with a Nitrosomonas-specific probe. Lakewater and sediment samples were analysed using a nested PCR technique in which eubacterial rRNA genes were subjected to a secondary amplification with Nitrosomonas or Nitrosospira specific primers. Again, the presence of Nitrosospira DNA, but not Nitrosomonas DNA, was detected and this was confirmed by hybridization of the amplified DNA with an internal oligonucleotide probe. Enrichments of lakewater and sediment samples, incubated for two weeks in the presence of ammonium, produced nitrite and were found to contain DNA from both Nitrosospira and Nitrosomonas as determined by nested PCR amplification and probing of 16S rRNA genes. This demonstrates that Nitrosospira spp. are widespread in the environment. The implications of the detection of Nitrosomonas DNA only after enrichment culture are discussed.",

keywords = "16S ribosomal RNA, Ammonia-oxidation, Nitrification, Nitrosomonas, Nitrosospira",

author = "Hiorns, {W. D.} and Hastings, {R. C.} and Head, {I. M.} and McCarthy, {A. J.} and Saunders, {J. R.} and Pickup, {R. W.} and Hall, {G. H.}",

year = "1995",

month = nov,

day = "1",

doi = "10.1099/13500872-141-11-2793",

language = "English",

volume = "141",

pages = "2793--2800",

journal = "Microbiology",

issn = "1350-0872",

publisher = "Society for General Microbiology",

number = "11",

}

RIS

TY - JOUR

T1 - Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment

AU - Hiorns, W. D.

AU - Hastings, R. C.

AU - Head, I. M.

AU - McCarthy, A. J.

AU - Saunders, J. R.

AU - Pickup, R. W.

AU - Hall, G. H.

PY - 1995/11/1

Y1 - 1995/11/1

N2 - Oligonucleotide sequences selected from the 16S rRNA genes of various species of ammonia-oxidizing bacteria were evaluated as specific PCR amplification primers and probes. The specificities of primer pairs for eubacterial, Nitrosospira and Nitrosomonas rRNA genes were established with sequence databases, and the primer pairs were used to amplify DNA from laboratory cultures and environmental samples. Eubacterial rRNA genes amplified from samples of soil and activated sludge hybridized with an oligonucleotide probe specific for Nitrosospira spp., but not with a Nitrosomonas-specific probe. Lakewater and sediment samples were analysed using a nested PCR technique in which eubacterial rRNA genes were subjected to a secondary amplification with Nitrosomonas or Nitrosospira specific primers. Again, the presence of Nitrosospira DNA, but not Nitrosomonas DNA, was detected and this was confirmed by hybridization of the amplified DNA with an internal oligonucleotide probe. Enrichments of lakewater and sediment samples, incubated for two weeks in the presence of ammonium, produced nitrite and were found to contain DNA from both Nitrosospira and Nitrosomonas as determined by nested PCR amplification and probing of 16S rRNA genes. This demonstrates that Nitrosospira spp. are widespread in the environment. The implications of the detection of Nitrosomonas DNA only after enrichment culture are discussed.

AB - Oligonucleotide sequences selected from the 16S rRNA genes of various species of ammonia-oxidizing bacteria were evaluated as specific PCR amplification primers and probes. The specificities of primer pairs for eubacterial, Nitrosospira and Nitrosomonas rRNA genes were established with sequence databases, and the primer pairs were used to amplify DNA from laboratory cultures and environmental samples. Eubacterial rRNA genes amplified from samples of soil and activated sludge hybridized with an oligonucleotide probe specific for Nitrosospira spp., but not with a Nitrosomonas-specific probe. Lakewater and sediment samples were analysed using a nested PCR technique in which eubacterial rRNA genes were subjected to a secondary amplification with Nitrosomonas or Nitrosospira specific primers. Again, the presence of Nitrosospira DNA, but not Nitrosomonas DNA, was detected and this was confirmed by hybridization of the amplified DNA with an internal oligonucleotide probe. Enrichments of lakewater and sediment samples, incubated for two weeks in the presence of ammonium, produced nitrite and were found to contain DNA from both Nitrosospira and Nitrosomonas as determined by nested PCR amplification and probing of 16S rRNA genes. This demonstrates that Nitrosospira spp. are widespread in the environment. The implications of the detection of Nitrosomonas DNA only after enrichment culture are discussed.

KW - 16S ribosomal RNA

KW - Ammonia-oxidation

KW - Nitrification

KW - Nitrosomonas

KW - Nitrosospira

U2 - 10.1099/13500872-141-11-2793

DO - 10.1099/13500872-141-11-2793

M3 - Journal article

C2 - 8535507

AN - SCOPUS:0028883636

VL - 141

SP - 2793

EP - 2800

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 11

ER -

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