This work proposes an atmospheric pressure plasma jet operated by the injection of natural air. The conventional plasma jet has been modified by creating a small hole in the quartz tube. The small amount of argon gas flow inside the tube creates a pressure difference with the surrounding environment. By Bernoulli's principle, natural air is forced to penetrate inside the tube to equalize this difference. The operational range of this device exists for only low argon gas flow rates of 200–600 sccm at a specified value of applied voltage. Compared to the conventional plasma jet without a hole, the concentration of reactive nitrogen species is significantly increased due to the mixing of the surrounding air. In addition, the gas temperature of the device remains close to room temperature, suggesting its strong possibility for biomedical applications. This approach can be an initiation towards the commercialization of plasma jets by using natural air.