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An In-depth Proteomic Map of Leishmania donovani Isolate from Post Kala-azar Dermal Leishmaniasis (PKDL) Patient

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An In-depth Proteomic Map of Leishmania donovani Isolate from Post Kala-azar Dermal Leishmaniasis (PKDL) Patient. / Routaray, Chinmayee Bar; Kumar, Avishek; Sundar, Shyam et al.

In: Acta Parasitologica, Vol. 67, No. 2, 30.06.2022, p. 687-696.

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Routaray, CB, Kumar, A, Sundar, S, Sathe, G, Pawar, H & Pai, K 2022, 'An In-depth Proteomic Map of Leishmania donovani Isolate from Post Kala-azar Dermal Leishmaniasis (PKDL) Patient', Acta Parasitologica, vol. 67, no. 2, pp. 687-696. https://doi.org/10.1007/s11686-021-00511-3

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Routaray CB, Kumar A, Sundar S, Sathe G, Pawar H, Pai K. An In-depth Proteomic Map of Leishmania donovani Isolate from Post Kala-azar Dermal Leishmaniasis (PKDL) Patient. Acta Parasitologica. 2022 Jun 30;67(2):687-696. Epub 2022 Jan 12. doi: 10.1007/s11686-021-00511-3

Author

Routaray, Chinmayee Bar ; Kumar, Avishek ; Sundar, Shyam et al. / An In-depth Proteomic Map of Leishmania donovani Isolate from Post Kala-azar Dermal Leishmaniasis (PKDL) Patient. In: Acta Parasitologica. 2022 ; Vol. 67, No. 2. pp. 687-696.

Bibtex

@article{b34c01c52ffe486fac4655d257d93699,
title = "An In-depth Proteomic Map of Leishmania donovani Isolate from Post Kala-azar Dermal Leishmaniasis (PKDL) Patient",
abstract = "Background: The trypanosomatid protozoan parasite Leishmania donovani is the etiological agent of visceral leishmaniasis (VL) or kala-azar. The patients that have undergone treatment may still harbor the parasite and in a small fraction of the patients the disease re-erupts in the form of post kala-azar dermal leishmaniasis (PKDL). PKDL is a pathological condition found to be intermediate between VL and complete cure of VL. The PKDL disease progression is determined by the host immune response to L. donovani. The majority of the proteomic studies on L. donovani till date have been undertaken on parasites either isolated from kala-azar patients or on established laboratory strains of L. donovani. However, no proteomic information is available on the cutaneous localized isolates of L. donovani from PKDL patients.Methods: The promastigote stage of L. donovani isolate from PKDL patient was cultured and harvested. The cell lysates were trypsin digested, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The LC-MS/MS raw data were analyzed on Proteome Discoverer. Further bioinformatics analysis was carried out.Results: In the present, we have used high-resolution mass spectrometry to map the global proteome of a L. donovani isolate from PKDL patient. This in-depth study resulted in the identification of 5537 unique proteins from PKDL isolate of L. donovani which covered 64% of its proteome.Outcome: This study also identified proteins previously shown to be upregulated in PKDL L. donovani. This is the most in-depth proteome of Leishmania donovani parasite till date.",
author = "Routaray, {Chinmayee Bar} and Avishek Kumar and Shyam Sundar and Gajanan Sathe and Harsh Pawar and Kalpana Pai",
year = "2022",
month = jun,
day = "30",
doi = "10.1007/s11686-021-00511-3",
language = "English",
volume = "67",
pages = "687--696",
journal = "Acta Parasitologica",
issn = "1230-2821",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - An In-depth Proteomic Map of Leishmania donovani Isolate from Post Kala-azar Dermal Leishmaniasis (PKDL) Patient

AU - Routaray, Chinmayee Bar

AU - Kumar, Avishek

AU - Sundar, Shyam

AU - Sathe, Gajanan

AU - Pawar, Harsh

AU - Pai, Kalpana

PY - 2022/6/30

Y1 - 2022/6/30

N2 - Background: The trypanosomatid protozoan parasite Leishmania donovani is the etiological agent of visceral leishmaniasis (VL) or kala-azar. The patients that have undergone treatment may still harbor the parasite and in a small fraction of the patients the disease re-erupts in the form of post kala-azar dermal leishmaniasis (PKDL). PKDL is a pathological condition found to be intermediate between VL and complete cure of VL. The PKDL disease progression is determined by the host immune response to L. donovani. The majority of the proteomic studies on L. donovani till date have been undertaken on parasites either isolated from kala-azar patients or on established laboratory strains of L. donovani. However, no proteomic information is available on the cutaneous localized isolates of L. donovani from PKDL patients.Methods: The promastigote stage of L. donovani isolate from PKDL patient was cultured and harvested. The cell lysates were trypsin digested, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The LC-MS/MS raw data were analyzed on Proteome Discoverer. Further bioinformatics analysis was carried out.Results: In the present, we have used high-resolution mass spectrometry to map the global proteome of a L. donovani isolate from PKDL patient. This in-depth study resulted in the identification of 5537 unique proteins from PKDL isolate of L. donovani which covered 64% of its proteome.Outcome: This study also identified proteins previously shown to be upregulated in PKDL L. donovani. This is the most in-depth proteome of Leishmania donovani parasite till date.

AB - Background: The trypanosomatid protozoan parasite Leishmania donovani is the etiological agent of visceral leishmaniasis (VL) or kala-azar. The patients that have undergone treatment may still harbor the parasite and in a small fraction of the patients the disease re-erupts in the form of post kala-azar dermal leishmaniasis (PKDL). PKDL is a pathological condition found to be intermediate between VL and complete cure of VL. The PKDL disease progression is determined by the host immune response to L. donovani. The majority of the proteomic studies on L. donovani till date have been undertaken on parasites either isolated from kala-azar patients or on established laboratory strains of L. donovani. However, no proteomic information is available on the cutaneous localized isolates of L. donovani from PKDL patients.Methods: The promastigote stage of L. donovani isolate from PKDL patient was cultured and harvested. The cell lysates were trypsin digested, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The LC-MS/MS raw data were analyzed on Proteome Discoverer. Further bioinformatics analysis was carried out.Results: In the present, we have used high-resolution mass spectrometry to map the global proteome of a L. donovani isolate from PKDL patient. This in-depth study resulted in the identification of 5537 unique proteins from PKDL isolate of L. donovani which covered 64% of its proteome.Outcome: This study also identified proteins previously shown to be upregulated in PKDL L. donovani. This is the most in-depth proteome of Leishmania donovani parasite till date.

U2 - 10.1007/s11686-021-00511-3

DO - 10.1007/s11686-021-00511-3

M3 - Journal article

VL - 67

SP - 687

EP - 696

JO - Acta Parasitologica

JF - Acta Parasitologica

SN - 1230-2821

IS - 2

ER -