Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - An investigation of ribulosebisphosphate carboxylase activity by high resolution 1H NMR
AU - Gutteridge, Steven
AU - Parry, Martin A J
AU - Schmidt, C. N Godfrey
AU - Feeney, Jim
PY - 1984/5/21
Y1 - 1984/5/21
N2 - The reactions catalysed by ribulosebisphosphate carboxylase/oxygenase have been studied by high resolution proton NMR spectroscopy. The protons of the substrate ribulose-P2 and the products of the carboxylase and oxygenase reactions are readily assigned to the various carbon centres. Half of the 3-phosphoglycerate molecules generated during carboxylase turnover of ribulose-P2 in saturating CO2 concentrations acquire deuterium from the solution at the C2 position, whereas the other 3-phosphoglycerate molecules and also the 3-phosphoglycerate produced as a result of the oxygenase activity contain no deuterium of this type. However, the addition of activated enzyme (i.e., in the presence of an effector) to a solution of ribulose-P2 in the near absence of either CO2 or O2 catalyses the exchange of the C3 proton with the deuterium of the solution.
AB - The reactions catalysed by ribulosebisphosphate carboxylase/oxygenase have been studied by high resolution proton NMR spectroscopy. The protons of the substrate ribulose-P2 and the products of the carboxylase and oxygenase reactions are readily assigned to the various carbon centres. Half of the 3-phosphoglycerate molecules generated during carboxylase turnover of ribulose-P2 in saturating CO2 concentrations acquire deuterium from the solution at the C2 position, whereas the other 3-phosphoglycerate molecules and also the 3-phosphoglycerate produced as a result of the oxygenase activity contain no deuterium of this type. However, the addition of activated enzyme (i.e., in the presence of an effector) to a solution of ribulose-P2 in the near absence of either CO2 or O2 catalyses the exchange of the C3 proton with the deuterium of the solution.
KW - Enediol
KW - NMR
KW - Proton/deuterium exchange
KW - Ribulose bisphosphate
KW - Ribulosebisphosphate carboxylase/oxygenase
UR - http://www.scopus.com/inward/record.url?scp=0005651561&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(84)81343-5
DO - 10.1016/0014-5793(84)81343-5
M3 - Journal article
AN - SCOPUS:0005651561
VL - 170
SP - 355
EP - 359
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2
ER -