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Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis. / Robinson, Nicholas P.
In: Methods in Molecular Biology, Vol. 1054, 17.07.2013, p. 45-61.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Robinson, NP 2013, 'Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis', Methods in Molecular Biology, vol. 1054, pp. 45-61. https://doi.org/10.1007/978-1-62703-565-1_3

APA

Robinson, N. P. (2013). Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis. Methods in Molecular Biology, 1054, 45-61. https://doi.org/10.1007/978-1-62703-565-1_3

Vancouver

Robinson NP. Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis. Methods in Molecular Biology. 2013 Jul 17;1054:45-61. doi: 10.1007/978-1-62703-565-1_3

Author

Robinson, Nicholas P. / Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis. In: Methods in Molecular Biology. 2013 ; Vol. 1054. pp. 45-61.

Bibtex

@article{87fb84cb46524496a3655c856080a844,
title = "Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis",
abstract = "Branched DNA molecules are generated by the essential processes of replication and recombination. Owing to their distinctive extended shapes, these intermediates migrate differently from linear double-stranded DNA under certain electrophoretic conditions. However, these branched species exist in the cell at much low abundance than the bulk linear DNA. Consequently, branched molecules cannot be visualized by conventional electrophoresis and ethidium bromide staining. Two-dimensional native-native agarose electrophoresis has therefore been developed as a method to facilitate the separation and visualization of branched replication and recombination intermediates. A wide variety of studies have employed this technique to examine branched molecules in eukaryotic, archaeal, and bacterial cells, providing valuable insights into how DNA is duplicated and repaired in all three domains of life.",
keywords = "DNA Replication, Electrophoresis, Gel, Two-Dimensional, Eukaryotic Cells, Prokaryotic Cells, Recombination, Genetic, Journal Article",
author = "Robinson, {Nicholas P.}",
year = "2013",
month = jul,
day = "17",
doi = "10.1007/978-1-62703-565-1_3",
language = "English",
volume = "1054",
pages = "45--61",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis

AU - Robinson, Nicholas P.

PY - 2013/7/17

Y1 - 2013/7/17

N2 - Branched DNA molecules are generated by the essential processes of replication and recombination. Owing to their distinctive extended shapes, these intermediates migrate differently from linear double-stranded DNA under certain electrophoretic conditions. However, these branched species exist in the cell at much low abundance than the bulk linear DNA. Consequently, branched molecules cannot be visualized by conventional electrophoresis and ethidium bromide staining. Two-dimensional native-native agarose electrophoresis has therefore been developed as a method to facilitate the separation and visualization of branched replication and recombination intermediates. A wide variety of studies have employed this technique to examine branched molecules in eukaryotic, archaeal, and bacterial cells, providing valuable insights into how DNA is duplicated and repaired in all three domains of life.

AB - Branched DNA molecules are generated by the essential processes of replication and recombination. Owing to their distinctive extended shapes, these intermediates migrate differently from linear double-stranded DNA under certain electrophoretic conditions. However, these branched species exist in the cell at much low abundance than the bulk linear DNA. Consequently, branched molecules cannot be visualized by conventional electrophoresis and ethidium bromide staining. Two-dimensional native-native agarose electrophoresis has therefore been developed as a method to facilitate the separation and visualization of branched replication and recombination intermediates. A wide variety of studies have employed this technique to examine branched molecules in eukaryotic, archaeal, and bacterial cells, providing valuable insights into how DNA is duplicated and repaired in all three domains of life.

KW - DNA Replication

KW - Electrophoresis, Gel, Two-Dimensional

KW - Eukaryotic Cells

KW - Prokaryotic Cells

KW - Recombination, Genetic

KW - Journal Article

U2 - 10.1007/978-1-62703-565-1_3

DO - 10.1007/978-1-62703-565-1_3

M3 - Journal article

C2 - 23913284

VL - 1054

SP - 45

EP - 61

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -