Home > Research > Publications & Outputs > Analysis of Rad3 and Chk1 protein kinases defin...

Electronic data

Links

Text available via DOI:

View graph of relations

Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses.

Research output: Contribution to journalJournal articlepeer-review

Published

Standard

Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. / Martinho, Rui G.; Lindsay, Howard D.; Flaggs, Gail; DeMaggio, Anthony J; Hoekstra, Merl F.; Carr, Antony M.; Bentley, Nicola J.

In: EMBO Journal, Vol. 17, No. 24, 15.12.1998, p. 7239-7249.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Martinho, RG, Lindsay, HD, Flaggs, G, DeMaggio, AJ, Hoekstra, MF, Carr, AM & Bentley, NJ 1998, 'Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses.', EMBO Journal, vol. 17, no. 24, pp. 7239-7249. https://doi.org/10.1093/emboj/17.24.7239

APA

Martinho, R. G., Lindsay, H. D., Flaggs, G., DeMaggio, A. J., Hoekstra, M. F., Carr, A. M., & Bentley, N. J. (1998). Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. EMBO Journal, 17(24), 7239-7249. https://doi.org/10.1093/emboj/17.24.7239

Vancouver

Martinho RG, Lindsay HD, Flaggs G, DeMaggio AJ, Hoekstra MF, Carr AM et al. Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. EMBO Journal. 1998 Dec 15;17(24):7239-7249. https://doi.org/10.1093/emboj/17.24.7239

Author

Martinho, Rui G. ; Lindsay, Howard D. ; Flaggs, Gail ; DeMaggio, Anthony J ; Hoekstra, Merl F. ; Carr, Antony M. ; Bentley, Nicola J. / Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. In: EMBO Journal. 1998 ; Vol. 17, No. 24. pp. 7239-7249.

Bibtex

@article{2b3c3268be84465c96794cbb9ea8839e,
title = "Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses.",
abstract = "Eukaryotic cells respond to DNA damage and S phase replication blocks by arresting cell-cycle progression through the DNA structure checkpoint pathways. In Schizosaccharomyces pombe, the Chk1 kinase is essential for mitotic arrest and is phosphorylated after DNA damage. During S phase, the Cds1 kinase is activated in response to DNA damage and DNA replication blocks. The response of both Chk1 and Cds1 requires the six 'checkpoint Rad' proteins (Rad1, Rad3, Rad9, Rad17, Rad26 and Hus1). We demonstrate that DNA damage-dependent phosphorylation of Chk1 is also cell-cycle specific, occurring primarily in late S phase and G2, but not during M/G1 or early S phase. We have also isolated and characterized a temperature-sensitive allele of rad3. Rad3 functions differently depending on which checkpoint pathway is activated. Following DNA damage, rad3 is required to initiate but not maintain the Chk1 response. When DNA replication is inhibited, rad3 is required for both initiation and maintenance of the Cds1 response. We have identified a strong genetic interaction between rad3 and cds1, and biochemical evidence shows a physical interaction is possible between Rad3 and Cds1, and between Rad3 and Chk1 in vitro. Together, our results highlight the cell-cycle specificity of the DNA structure-dependent checkpoint response and identify distinct roles for Rad3 in the different checkpoint responses.",
keywords = "ATM, ATR, cell-cycle checkpoints, Chk1, Rad3",
author = "Martinho, {Rui G.} and Lindsay, {Howard D.} and Gail Flaggs and DeMaggio, {Anthony J} and Hoekstra, {Merl F.} and Carr, {Antony M.} and Bentley, {Nicola J.}",
year = "1998",
month = dec,
day = "15",
doi = "10.1093/emboj/17.24.7239",
language = "English",
volume = "17",
pages = "7239--7249",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "24",

}

RIS

TY - JOUR

T1 - Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses.

AU - Martinho, Rui G.

AU - Lindsay, Howard D.

AU - Flaggs, Gail

AU - DeMaggio, Anthony J

AU - Hoekstra, Merl F.

AU - Carr, Antony M.

AU - Bentley, Nicola J.

PY - 1998/12/15

Y1 - 1998/12/15

N2 - Eukaryotic cells respond to DNA damage and S phase replication blocks by arresting cell-cycle progression through the DNA structure checkpoint pathways. In Schizosaccharomyces pombe, the Chk1 kinase is essential for mitotic arrest and is phosphorylated after DNA damage. During S phase, the Cds1 kinase is activated in response to DNA damage and DNA replication blocks. The response of both Chk1 and Cds1 requires the six 'checkpoint Rad' proteins (Rad1, Rad3, Rad9, Rad17, Rad26 and Hus1). We demonstrate that DNA damage-dependent phosphorylation of Chk1 is also cell-cycle specific, occurring primarily in late S phase and G2, but not during M/G1 or early S phase. We have also isolated and characterized a temperature-sensitive allele of rad3. Rad3 functions differently depending on which checkpoint pathway is activated. Following DNA damage, rad3 is required to initiate but not maintain the Chk1 response. When DNA replication is inhibited, rad3 is required for both initiation and maintenance of the Cds1 response. We have identified a strong genetic interaction between rad3 and cds1, and biochemical evidence shows a physical interaction is possible between Rad3 and Cds1, and between Rad3 and Chk1 in vitro. Together, our results highlight the cell-cycle specificity of the DNA structure-dependent checkpoint response and identify distinct roles for Rad3 in the different checkpoint responses.

AB - Eukaryotic cells respond to DNA damage and S phase replication blocks by arresting cell-cycle progression through the DNA structure checkpoint pathways. In Schizosaccharomyces pombe, the Chk1 kinase is essential for mitotic arrest and is phosphorylated after DNA damage. During S phase, the Cds1 kinase is activated in response to DNA damage and DNA replication blocks. The response of both Chk1 and Cds1 requires the six 'checkpoint Rad' proteins (Rad1, Rad3, Rad9, Rad17, Rad26 and Hus1). We demonstrate that DNA damage-dependent phosphorylation of Chk1 is also cell-cycle specific, occurring primarily in late S phase and G2, but not during M/G1 or early S phase. We have also isolated and characterized a temperature-sensitive allele of rad3. Rad3 functions differently depending on which checkpoint pathway is activated. Following DNA damage, rad3 is required to initiate but not maintain the Chk1 response. When DNA replication is inhibited, rad3 is required for both initiation and maintenance of the Cds1 response. We have identified a strong genetic interaction between rad3 and cds1, and biochemical evidence shows a physical interaction is possible between Rad3 and Cds1, and between Rad3 and Chk1 in vitro. Together, our results highlight the cell-cycle specificity of the DNA structure-dependent checkpoint response and identify distinct roles for Rad3 in the different checkpoint responses.

KW - ATM

KW - ATR

KW - cell-cycle checkpoints

KW - Chk1

KW - Rad3

U2 - 10.1093/emboj/17.24.7239

DO - 10.1093/emboj/17.24.7239

M3 - Journal article

VL - 17

SP - 7239

EP - 7249

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 24

ER -