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Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms

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Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms. / Bloemers, Duncan; Carmo-Silva, Elizabete.
Photosynthesis: Methods and Protocols. ed. / Sarah Covshoff. New York: Humana Press, 2024. p. 405-416 (Methods in Molecular Biology; Vol. 2790).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter

Harvard

Bloemers, D & Carmo-Silva, E 2024, Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms. in S Covshoff (ed.), Photosynthesis: Methods and Protocols. Methods in Molecular Biology, vol. 2790, Humana Press, New York, pp. 405-416. https://doi.org/10.1007/978-1-0716-3790-6_21

APA

Bloemers, D., & Carmo-Silva, E. (2024). Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms. In S. Covshoff (Ed.), Photosynthesis: Methods and Protocols (pp. 405-416). (Methods in Molecular Biology; Vol. 2790). Humana Press. https://doi.org/10.1007/978-1-0716-3790-6_21

Vancouver

Bloemers D, Carmo-Silva E. Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms. In Covshoff S, editor, Photosynthesis: Methods and Protocols. New York: Humana Press. 2024. p. 405-416. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-3790-6_21

Author

Bloemers, Duncan ; Carmo-Silva, Elizabete. / Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms. Photosynthesis: Methods and Protocols. editor / Sarah Covshoff. New York : Humana Press, 2024. pp. 405-416 (Methods in Molecular Biology).

Bibtex

@inbook{80bbbb414a08461994ecdfd523e01566,
title = "Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms",
abstract = "Antibodies are a valuable research tool, with uses including detection and quantification of specific proteins. By using peptide fragments to raise antibodies, they can be designed to differentiate between structurally similar proteins, or to bind conserved motifs in divergent proteins. Peptide sequence selection and antibody validation are crucial to ensure reliable results from antibody-based experiments. This chapter describes the steps for the identification of peptide sequences to produce protein- or isoform-specific antibodies using recombinant technologies as well as the subsequent validation of such antibodies. The photosynthetic protein Rubisco activase is used as a case study to explain the various steps involved and key aspects to take into consideration.",
keywords = "Antibodies - chemistry - immunology - metabolism, Immunoblotting, Photosynthesis, Plant Proteins - metabolism, Protein Isoforms, Antibody design, Monospecific antibody, Protein detection, Protein quantification, Recombinant antibody, Amino Acid Sequence, Antibody validation, Rubisco activase, Antibody production",
author = "Duncan Bloemers and Elizabete Carmo-Silva",
year = "2024",
month = apr,
day = "23",
doi = "10.1007/978-1-0716-3790-6_21",
language = "English",
isbn = "9781071637920",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "405--416",
editor = "Sarah Covshoff",
booktitle = "Photosynthesis",

}

RIS

TY - CHAP

T1 - Antibody Design for the Quantification of Photosynthetic Proteins and Their Isoforms

AU - Bloemers, Duncan

AU - Carmo-Silva, Elizabete

PY - 2024/4/23

Y1 - 2024/4/23

N2 - Antibodies are a valuable research tool, with uses including detection and quantification of specific proteins. By using peptide fragments to raise antibodies, they can be designed to differentiate between structurally similar proteins, or to bind conserved motifs in divergent proteins. Peptide sequence selection and antibody validation are crucial to ensure reliable results from antibody-based experiments. This chapter describes the steps for the identification of peptide sequences to produce protein- or isoform-specific antibodies using recombinant technologies as well as the subsequent validation of such antibodies. The photosynthetic protein Rubisco activase is used as a case study to explain the various steps involved and key aspects to take into consideration.

AB - Antibodies are a valuable research tool, with uses including detection and quantification of specific proteins. By using peptide fragments to raise antibodies, they can be designed to differentiate between structurally similar proteins, or to bind conserved motifs in divergent proteins. Peptide sequence selection and antibody validation are crucial to ensure reliable results from antibody-based experiments. This chapter describes the steps for the identification of peptide sequences to produce protein- or isoform-specific antibodies using recombinant technologies as well as the subsequent validation of such antibodies. The photosynthetic protein Rubisco activase is used as a case study to explain the various steps involved and key aspects to take into consideration.

KW - Antibodies - chemistry - immunology - metabolism

KW - Immunoblotting

KW - Photosynthesis

KW - Plant Proteins - metabolism

KW - Protein Isoforms

KW - Antibody design

KW - Monospecific antibody

KW - Protein detection

KW - Protein quantification

KW - Recombinant antibody

KW - Amino Acid Sequence

KW - Antibody validation

KW - Rubisco activase

KW - Antibody production

U2 - 10.1007/978-1-0716-3790-6_21

DO - 10.1007/978-1-0716-3790-6_21

M3 - Chapter

C2 - 38649583

SN - 9781071637920

SN - 9781071637890

T3 - Methods in Molecular Biology

SP - 405

EP - 416

BT - Photosynthesis

A2 - Covshoff, Sarah

PB - Humana Press

CY - New York

ER -