Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Antifungal bioassay-guided fractionation of an oil extract of propolis
AU - Finger, Daiane
AU - Machado, Christiane Schinieder
AU - Torres, Yohandra Reyes
AU - Quináia, Sueli Percio
AU - Thomaz, Amanda Cristina Godot
AU - Gobbo, Angélica Rita
AU - Monteiro, Marta Chagas
AU - Ferreira, Antonio Gilberto
AU - Sawaya, Alexandra Christine Helena Frankland
AU - Eberlin, Marcos Nogueira
PY - 2013/10/31
Y1 - 2013/10/31
N2 - The study aimed to evaluate the antifungal potential of an extract of propolis obtained with edible vegetable oil (ODEP) and to identify antifungal compounds in this extract. Propolis sample was extracted with canola oil. After filtration, the oily liquid extract was submitted to solvent partition and dried to obtain ODEP, which was tested in vitro against Candida albicans strains. ODEP was fractioned on Sephadex and by high-performance liquid chromatography (HPLC). Active fractions and/or a pure compound were analyzed by nuclear magnetic resonance, liquid chromatographic mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to characterize their chemical composition. Bioassay-guided fractionation allowed the isolation of dihydrokaempferide, which was also quantified in ODEP by HPLC together with the total flavonoid content. Kaempferide and isosakuranetin were identified by LC-MS and LC-MS/MS in fractions with a potential activity against C.albicans. In vitro assays showed that some fractions from ODEP produced better inhibition of C.albicans than the propolis extract itself.
AB - The study aimed to evaluate the antifungal potential of an extract of propolis obtained with edible vegetable oil (ODEP) and to identify antifungal compounds in this extract. Propolis sample was extracted with canola oil. After filtration, the oily liquid extract was submitted to solvent partition and dried to obtain ODEP, which was tested in vitro against Candida albicans strains. ODEP was fractioned on Sephadex and by high-performance liquid chromatography (HPLC). Active fractions and/or a pure compound were analyzed by nuclear magnetic resonance, liquid chromatographic mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to characterize their chemical composition. Bioassay-guided fractionation allowed the isolation of dihydrokaempferide, which was also quantified in ODEP by HPLC together with the total flavonoid content. Kaempferide and isosakuranetin were identified by LC-MS and LC-MS/MS in fractions with a potential activity against C.albicans. In vitro assays showed that some fractions from ODEP produced better inhibition of C.albicans than the propolis extract itself.
U2 - 10.1111/jfq.12039
DO - 10.1111/jfq.12039
M3 - Journal article
AN - SCOPUS:84885062343
VL - 36
SP - 291
EP - 301
JO - Journal of Food Quality
JF - Journal of Food Quality
SN - 0146-9428
IS - 5
ER -