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Antiphospholipid antibodies prevent extravillous trophoblast differentiation.

Research output: Contribution to journalJournal articlepeer-review

Published
  • Siobhan Quenby
  • Steve Mountfield
  • Judith E. Cartwright
  • Guy StJ. Whitley
  • Larry Chamley
  • Gill S. Vince
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<mark>Journal publication date</mark>1/03/2005
<mark>Journal</mark>Fertility and Sterility
Issue number3
Volume83
Number of pages8
Pages (from-to)691-698
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Objective We investigated the hypothesis that antiphospholipid antibodies (aPL) have a detrimental effect on human extravillous trophoblast (EVT) differentiation into giant multinucleated cells “in vitro.” Design The EVT were isolated from the placental chorion using enzymatic digestion and Percoll gradient centrifugation. After 24, 36, and 48 hours in culture, giant multinuclear cells (GMC) were identified by immunohistochemistry using antibodies to cytokeratin 7 and counted. Setting An academic research laboratory. Patient(s) Placentas were donated by women having an elective cesarean section for a normal pregnancy at term. Main outcome measure(s) This model was then used to investigate the effect of two different monoclonal aPL to β2-glycoprotein 1 (IIC5 and ID2), and control mouse IgG antibody on EVT differentiation. Result(s) Freshly isolated EVT were nonproliferative but moved together losing their intervening cell walls and differentiated into GMC. Maximal numbers of GMC were detected after 48 hours of culture. The aPL, IIC5, and ID2 significantly inhibited GMC formation, whereas the mouse IgG control had no effect. Conclusion(s) Antiphospholipid antibodies can inhibit EVT differentiation and GMC formation “in vitro” suggesting that a failure of trophoblast differentiation and subsequent uteroplacental development may be an underlying pathology in antiphospholipid syndrome-associated pregnancy loss.

Bibliographic note

RAE_import_type : Journal article RAE_uoa_type : Allied Health Professions and Studies