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Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem

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Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem. / Bogus, K.; Żarczyńska, M.; Pałasz, A. et al.
In: Molecular Biology Reports, Vol. 49, No. 2, 28.02.2022, p. 1601-1608.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Bogus, K, Żarczyńska, M, Pałasz, A, Suszka-Świtek, A, Worthington, JJ, Krzystanek, M & Żarczyński, P 2022, 'Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem', Molecular Biology Reports, vol. 49, no. 2, pp. 1601-1608. https://doi.org/10.1007/s11033-021-06943-4

APA

Bogus, K., Żarczyńska, M., Pałasz, A., Suszka-Świtek, A., Worthington, J. J., Krzystanek, M., & Żarczyński, P. (2022). Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem. Molecular Biology Reports, 49(2), 1601-1608. https://doi.org/10.1007/s11033-021-06943-4

Vancouver

Bogus K, Żarczyńska M, Pałasz A, Suszka-Świtek A, Worthington JJ, Krzystanek M et al. Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem. Molecular Biology Reports. 2022 Feb 28;49(2):1601-1608. Epub 2021 Nov 19. doi: 10.1007/s11033-021-06943-4

Author

Bogus, K. ; Żarczyńska, M. ; Pałasz, A. et al. / Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem. In: Molecular Biology Reports. 2022 ; Vol. 49, No. 2. pp. 1601-1608.

Bibtex

@article{f9134b8de4c942f7baf17c8007c77f42,
title = "Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem",
abstract = "Background: Neurosteroids are involved in several important brain functions and have recently been considered novel players in the mechanic actions of neuropsychiatric drugs. There are no reports of murine studies focusing on the effect of chronic neurosteroid treatment in parallel with antipsychotics on key steroidogenic enzyme expression and we therefore focused on steroidogenic enzyme gene expression in the brainstem of rats chronically treated with olanzapine and haloperidol. Methods and results: Studies were carried out on adult, male Sprague–Dawley rats which were divided into 3 groups: control and experimental animals treated with olanzapine or haloperidol. Total mRNA was isolated from homogenized brainstem samples for RealTime-PCR to estimate gene expression of related aromatase, 3β-HSD and P450scc. Long-term treatment with the selected antipsychotics was reflected in the modulation of steroidogenic enzyme gene expression in the examined brainstem region; with both olanzapine and haloperidol increasing aromatase, 3β-HSD and P450scc gene expression. Conclusions: The present findings shed new light on the pharmacology of antipsychotics and suggest the existence of possible regulatory interplay between neuroleptic action and steroidogenesis at the level of brainstem neuronal centres. ",
keywords = "3β-HSD, Aromatase, Haloperidol, Neurosteroids, Olanzapine, P450scc",
author = "K. Bogus and M. {\.Z}arczy{\'n}ska and A. Pa{\l}asz and A. Suszka-{\'S}witek and J.J. Worthington and M. Krzystanek and P. {\.Z}arczy{\'n}ski",
year = "2022",
month = feb,
day = "28",
doi = "10.1007/s11033-021-06943-4",
language = "English",
volume = "49",
pages = "1601--1608",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Antipsychotics increase steroidogenic enzyme gene expression in the rat brainstem

AU - Bogus, K.

AU - Żarczyńska, M.

AU - Pałasz, A.

AU - Suszka-Świtek, A.

AU - Worthington, J.J.

AU - Krzystanek, M.

AU - Żarczyński, P.

PY - 2022/2/28

Y1 - 2022/2/28

N2 - Background: Neurosteroids are involved in several important brain functions and have recently been considered novel players in the mechanic actions of neuropsychiatric drugs. There are no reports of murine studies focusing on the effect of chronic neurosteroid treatment in parallel with antipsychotics on key steroidogenic enzyme expression and we therefore focused on steroidogenic enzyme gene expression in the brainstem of rats chronically treated with olanzapine and haloperidol. Methods and results: Studies were carried out on adult, male Sprague–Dawley rats which were divided into 3 groups: control and experimental animals treated with olanzapine or haloperidol. Total mRNA was isolated from homogenized brainstem samples for RealTime-PCR to estimate gene expression of related aromatase, 3β-HSD and P450scc. Long-term treatment with the selected antipsychotics was reflected in the modulation of steroidogenic enzyme gene expression in the examined brainstem region; with both olanzapine and haloperidol increasing aromatase, 3β-HSD and P450scc gene expression. Conclusions: The present findings shed new light on the pharmacology of antipsychotics and suggest the existence of possible regulatory interplay between neuroleptic action and steroidogenesis at the level of brainstem neuronal centres.

AB - Background: Neurosteroids are involved in several important brain functions and have recently been considered novel players in the mechanic actions of neuropsychiatric drugs. There are no reports of murine studies focusing on the effect of chronic neurosteroid treatment in parallel with antipsychotics on key steroidogenic enzyme expression and we therefore focused on steroidogenic enzyme gene expression in the brainstem of rats chronically treated with olanzapine and haloperidol. Methods and results: Studies were carried out on adult, male Sprague–Dawley rats which were divided into 3 groups: control and experimental animals treated with olanzapine or haloperidol. Total mRNA was isolated from homogenized brainstem samples for RealTime-PCR to estimate gene expression of related aromatase, 3β-HSD and P450scc. Long-term treatment with the selected antipsychotics was reflected in the modulation of steroidogenic enzyme gene expression in the examined brainstem region; with both olanzapine and haloperidol increasing aromatase, 3β-HSD and P450scc gene expression. Conclusions: The present findings shed new light on the pharmacology of antipsychotics and suggest the existence of possible regulatory interplay between neuroleptic action and steroidogenesis at the level of brainstem neuronal centres.

KW - 3β-HSD

KW - Aromatase

KW - Haloperidol

KW - Neurosteroids

KW - Olanzapine

KW - P450scc

U2 - 10.1007/s11033-021-06943-4

DO - 10.1007/s11033-021-06943-4

M3 - Journal article

VL - 49

SP - 1601

EP - 1608

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 2

ER -