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Asparaginase from the testa of developing lupin and pea seeds.

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Asparaginase from the testa of developing lupin and pea seeds. / Sodek, Ladaslav; Lea, Peter J.

In: Phytochemistry, Vol. 34, No. 1, 08.1993, p. 51-56.

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Sodek, Ladaslav ; Lea, Peter J. / Asparaginase from the testa of developing lupin and pea seeds. In: Phytochemistry. 1993 ; Vol. 34, No. 1. pp. 51-56.

Bibtex

@article{0835e1d93a964dfeab50989c221afd96,
title = "Asparaginase from the testa of developing lupin and pea seeds.",
abstract = "Potassium independent asparaginase has been purified from the testa of maturing seeds of Lupinus polyphyllus. The enzyme has a Mr of 71 × 103 and is composed of two subunits of Mr 35–36 × 103. Antisera raised against the purified asparaginase cross-reacted with a protein of similar characteristics isolated from the cotyledons and leaves of L. polyphyllus but no reaction was detected with extracts from the roots and nodules following Western blot analysis. Potassium dependent asparaginase was purified to a lesser extent from the testa of pea seeds and was shown to have a Mr of 61 × 103. The potassium independent and dependent enzymes appear to be distinct proteins with different immunological characteristics. The proportion of the two enzymes varies with plant species, organ and developmental age.",
keywords = "Lupinus polyphyllus, Pisum sativum, Leguminosae, asparaginase, immunology, potassium, testa.",
author = "Ladaslav Sodek and Lea, {Peter J.}",
year = "1993",
month = aug,
doi = "10.1016/S0031-9422(00)90781-7",
language = "English",
volume = "34",
pages = "51--56",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier Limited",
number = "1",

}

RIS

TY - JOUR

T1 - Asparaginase from the testa of developing lupin and pea seeds.

AU - Sodek, Ladaslav

AU - Lea, Peter J.

PY - 1993/8

Y1 - 1993/8

N2 - Potassium independent asparaginase has been purified from the testa of maturing seeds of Lupinus polyphyllus. The enzyme has a Mr of 71 × 103 and is composed of two subunits of Mr 35–36 × 103. Antisera raised against the purified asparaginase cross-reacted with a protein of similar characteristics isolated from the cotyledons and leaves of L. polyphyllus but no reaction was detected with extracts from the roots and nodules following Western blot analysis. Potassium dependent asparaginase was purified to a lesser extent from the testa of pea seeds and was shown to have a Mr of 61 × 103. The potassium independent and dependent enzymes appear to be distinct proteins with different immunological characteristics. The proportion of the two enzymes varies with plant species, organ and developmental age.

AB - Potassium independent asparaginase has been purified from the testa of maturing seeds of Lupinus polyphyllus. The enzyme has a Mr of 71 × 103 and is composed of two subunits of Mr 35–36 × 103. Antisera raised against the purified asparaginase cross-reacted with a protein of similar characteristics isolated from the cotyledons and leaves of L. polyphyllus but no reaction was detected with extracts from the roots and nodules following Western blot analysis. Potassium dependent asparaginase was purified to a lesser extent from the testa of pea seeds and was shown to have a Mr of 61 × 103. The potassium independent and dependent enzymes appear to be distinct proteins with different immunological characteristics. The proportion of the two enzymes varies with plant species, organ and developmental age.

KW - Lupinus polyphyllus

KW - Pisum sativum

KW - Leguminosae

KW - asparaginase

KW - immunology

KW - potassium

KW - testa.

U2 - 10.1016/S0031-9422(00)90781-7

DO - 10.1016/S0031-9422(00)90781-7

M3 - Journal article

VL - 34

SP - 51

EP - 56

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - 1

ER -