Research output: Contribution to Journal/Magazine › Journal article › peer-review
As-resistance in Laboratory reared Fl, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil. / Langdon, C. J.; Morgan, A. J.; Charnock, J. M. et al.
In: Environmental Pollution, Vol. 157, No. 11, 11.2009, p. 3114-3119.Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - As-resistance in Laboratory reared Fl, F2 and F3 generation offspring of the earthworm Lumbricus rubellus inhabiting an As-contaminated mine soil.
AU - Langdon, C. J.
AU - Morgan, A. J.
AU - Charnock, J. M.
AU - Semple, Kirk T.
AU - Lowe, C. N.
PY - 2009/11
Y1 - 2009/11
N2 - Previous studies provided no unequivocal evidence demonstrating that field populations of Lumbricus rubellus Hoffmeister (1843), exhibit genetically inherited resistance to As-toxicity. In this study F1, F2 and F3 generation offspring derived from adults inhabiting As-contaminated field soil were resistant when exposed to 2000 mg kg−1 sodium arsenate. The offspring of uncontaminated adults were not As-resistant. Cocoon viability was 80% for F1 and 82% for F2 offspring from As-contaminated adults and 59% in the F1 control population. High energy synchrotron analysis was used to determine whether ligand complexation of As differed in samples of: resistant mine-site adults, the resistant F1 and F2 offspring of the mine-site earthworms exposed to the LC25 sodium arsenate (700 mg kg−1) of the F1 parental generation; and adult L. rubellus from an uncontaminated site exposed to LC25 concentrations of sodium arsenate (50 mg kg−1). XANES and EXAFS indicated that As was present as a sulfur-coordinated species. As-resistance in F1, F2 and F3 offspring of the earthworm Lumbricus rubellus.
AB - Previous studies provided no unequivocal evidence demonstrating that field populations of Lumbricus rubellus Hoffmeister (1843), exhibit genetically inherited resistance to As-toxicity. In this study F1, F2 and F3 generation offspring derived from adults inhabiting As-contaminated field soil were resistant when exposed to 2000 mg kg−1 sodium arsenate. The offspring of uncontaminated adults were not As-resistant. Cocoon viability was 80% for F1 and 82% for F2 offspring from As-contaminated adults and 59% in the F1 control population. High energy synchrotron analysis was used to determine whether ligand complexation of As differed in samples of: resistant mine-site adults, the resistant F1 and F2 offspring of the mine-site earthworms exposed to the LC25 sodium arsenate (700 mg kg−1) of the F1 parental generation; and adult L. rubellus from an uncontaminated site exposed to LC25 concentrations of sodium arsenate (50 mg kg−1). XANES and EXAFS indicated that As was present as a sulfur-coordinated species. As-resistance in F1, F2 and F3 offspring of the earthworm Lumbricus rubellus.
KW - Earthworms
KW - Arsenic
KW - Exposure history
KW - Resistance
KW - Ligand-binding
U2 - 10.1016/j.envpol.2009.05.027
DO - 10.1016/j.envpol.2009.05.027
M3 - Journal article
VL - 157
SP - 3114
EP - 3119
JO - Environmental Pollution
JF - Environmental Pollution
SN - 0269-7491
IS - 11
ER -