TY - BOOK

T1 - Assessing toxicity of carbon based nanoparticles in cells and zebrafish by using biospectroscopy

AU - Li, Junyi

PY - 2016

Y1 - 2016

N2 - Raman and infrared (IR) spectroscopies provide detailed information about biologicalconstituents such as lipids, proteins, carbohydrates and DNA/RNA, etc. Based on this,these techniques can be used to differentiate cells and tissues, as well as employed as adiagnostic tool for detecting post-exposure biochemical alterations in toxicityassessment due to the induced changes of chemical composition and structurereflected by their spectral properties. Over the past few decades, Raman and IRspectroscopies with the development of more sophisticated instruments can providehigh-resolution spectral data from heterogeneous biological samples, which consistingof large amount of biochemical information, is complex. Therefore, computationalanalysis is employed to process and analyse the data for obtaining meaningfulinformation and getting deeper insight into the wavenumbers-related biochemicalalterations. Carbon-based nanoparticles (CNPs) are most widely used novelnanomaterials. With their widespread application, concerns emerge on their potentialrisk to the health of organism and human, and investigation on their possible toxicityis urgently required. This thesis is contributing to the toxicity assessment of CNPs byusing spectroscopic techniques coupled with computational analysis. Findings fromour projects indicated that this approach has the capability of detecting the CNPsinducedbiochemical alterations both in vitro and in vivo, which implies thattechniques involved in IR and Raman spectroscopy can provide a rapid and highlysensitive tool to detect minimal changes at the subcellular level.

AB - Raman and infrared (IR) spectroscopies provide detailed information about biologicalconstituents such as lipids, proteins, carbohydrates and DNA/RNA, etc. Based on this,these techniques can be used to differentiate cells and tissues, as well as employed as adiagnostic tool for detecting post-exposure biochemical alterations in toxicityassessment due to the induced changes of chemical composition and structurereflected by their spectral properties. Over the past few decades, Raman and IRspectroscopies with the development of more sophisticated instruments can providehigh-resolution spectral data from heterogeneous biological samples, which consistingof large amount of biochemical information, is complex. Therefore, computationalanalysis is employed to process and analyse the data for obtaining meaningfulinformation and getting deeper insight into the wavenumbers-related biochemicalalterations. Carbon-based nanoparticles (CNPs) are most widely used novelnanomaterials. With their widespread application, concerns emerge on their potentialrisk to the health of organism and human, and investigation on their possible toxicityis urgently required. This thesis is contributing to the toxicity assessment of CNPs byusing spectroscopic techniques coupled with computational analysis. Findings fromour projects indicated that this approach has the capability of detecting the CNPsinducedbiochemical alterations both in vitro and in vivo, which implies thattechniques involved in IR and Raman spectroscopy can provide a rapid and highlysensitive tool to detect minimal changes at the subcellular level.

M3 - Doctoral Thesis

PB - Lancaster University

ER -