Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Assessment of Anaerobic Digestate Amended with Wood Ash and Green Vegetable Matter and Impacts on Microbial Growth
AU - Isagba, Ebuwa S.
AU - Ajieh, Mike U.
AU - Oshoma, Cyprian E.
AU - Amenaghawon, Andrew
AU - Ogofure, Abraham
AU - Obatusin, Victoria
AU - Obuekwe, Ifeyinwa S.
AU - Tongo, Isioma
AU - Ihoeghian, Newton
AU - Edosa, Valerie I. O.
AU - Erhunmwunse, Nosakhare
AU - Lag-Brotons, A. J.
AU - Emokaro, Christopher
AU - Ezemonye, Lawrence I. N.
AU - Semple, Kirk T.
PY - 2023/9/30
Y1 - 2023/9/30
N2 - Anaerobic digestion (AD) is a waste management method worldwide, that results in biogas and digestate production. The digestate remains is a potential biofertilizer but may fall short of vital nutrients required for optimal plant growth, hence, requires amendment with other biomass residues. This study was aimed at investigating the microbial quality of digestate amended with wood ash and vegetable matter during treatment. Digestate from cattle rumen content (CR), food waste (FW), fruit waste (FRW) and their combinations were amended with wood ash, vegetable (green) matter and a blend of both. Amendment was carried out for 5 weeks, and samples were analyzed weekly. Enumeration and identification of bacteria and fungi were carried out on culture media. The rhizobacterial potential of the identified bacterial isolates was also investigated using standard microbiological procedures. The results showed high bacterial and fungal load in vegetable amended digestate from the first to the fourth week. Comparatively, cattle rumen content and food waste digestate amended with vegetables were highly significant, with mean values of 0.8 and 0.6, respectively. The identified bacterial isolates namely, Bacillus subtilis, Salmonella sp, Pseudomonas sp, Proteus sp, Enterobacter sp, Chromobacterium sp Bacillus spp. and Escherichia coli were found to be involved in the solubilization of phosphate, nitrogen fixation, ammonia production and induction of indole acetic acid. Specifically, feedstocks amended with vegetable (green) waste was found to have a significant influence on microbial growth (bacterial and fungal loads) between week 1 and 4. This suggests that digestate amendment is a crucial recipe for microbial growth which has considerable benefits in nutrient enhancement and increase in carbon levels.
AB - Anaerobic digestion (AD) is a waste management method worldwide, that results in biogas and digestate production. The digestate remains is a potential biofertilizer but may fall short of vital nutrients required for optimal plant growth, hence, requires amendment with other biomass residues. This study was aimed at investigating the microbial quality of digestate amended with wood ash and vegetable matter during treatment. Digestate from cattle rumen content (CR), food waste (FW), fruit waste (FRW) and their combinations were amended with wood ash, vegetable (green) matter and a blend of both. Amendment was carried out for 5 weeks, and samples were analyzed weekly. Enumeration and identification of bacteria and fungi were carried out on culture media. The rhizobacterial potential of the identified bacterial isolates was also investigated using standard microbiological procedures. The results showed high bacterial and fungal load in vegetable amended digestate from the first to the fourth week. Comparatively, cattle rumen content and food waste digestate amended with vegetables were highly significant, with mean values of 0.8 and 0.6, respectively. The identified bacterial isolates namely, Bacillus subtilis, Salmonella sp, Pseudomonas sp, Proteus sp, Enterobacter sp, Chromobacterium sp Bacillus spp. and Escherichia coli were found to be involved in the solubilization of phosphate, nitrogen fixation, ammonia production and induction of indole acetic acid. Specifically, feedstocks amended with vegetable (green) waste was found to have a significant influence on microbial growth (bacterial and fungal loads) between week 1 and 4. This suggests that digestate amendment is a crucial recipe for microbial growth which has considerable benefits in nutrient enhancement and increase in carbon levels.
KW - Anaerobic
KW - Biomass
KW - Compost
KW - Rhizobacteria
KW - Biofertilizer
KW - Nutrient
KW - Deficiency
KW - Availability
KW - Feedstock
U2 - 10.1007/s12649-023-02055-1
DO - 10.1007/s12649-023-02055-1
M3 - Journal article
VL - 14
SP - 3013
EP - 3025
JO - Waste and Biomass Valorization
JF - Waste and Biomass Valorization
SN - 1877-2641
IS - 9
ER -