Rights statement: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Mutagenesis following peer review. The definitive publisher-authenticated version Bethany L Patenall, Hollie J Hathaway, Maisem Laabei, Amber E Young, Naing T Thet, A Toby A Jenkins, Robert D Short, Sarah L Allinson, Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli, Mutagenesis 20021 36, 5, 380-387 is available online at: https://academic.oup.com/mutage/article/36/5/380/6359773
Accepted author manuscript, 512 KB, PDF document
Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli
AU - Patenall, Bethany L
AU - Hathaway, Hollie
AU - Laabei, Maisem
AU - Young, Amber E.
AU - Thet, Naing T.
AU - Jenkins, A.T.A.
AU - Short, Robert D
AU - Allinson, Sarah Louise
N1 - This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Mutagenesis following peer review. The definitive publisher-authenticated version Bethany L Patenall, Hollie J Hathaway, Maisem Laabei, Amber E Young, Naing T Thet, A Toby A Jenkins, Robert D Short, Sarah L Allinson, Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli, Mutagenesis 20021 36, 5, 380-387 is available online at: https://academic.oup.com/mutage/article/36/5/380/6359773
PY - 2021/9/30
Y1 - 2021/9/30
N2 - Abstract The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV-radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on Escherichia coli were assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test, respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, which did not resemble that for UV implying that CAP-produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences.
AB - Abstract The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV-radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on Escherichia coli were assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test, respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, which did not resemble that for UV implying that CAP-produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences.
KW - Antibiotics
KW - Mutation
KW - Ciprofloxacin
KW - DNA damage
KW - DNA fragmentation
KW - Mutagenesis
KW - Plasma
KW - Ultraviolet rays
KW - Escherichia coli
U2 - 10.1093/mutage/geab030
DO - 10.1093/mutage/geab030
M3 - Journal article
VL - 36
SP - 380
EP - 387
JO - Mutagenesis
JF - Mutagenesis
SN - 0267-8357
IS - 5
ER -