Final published version
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Attenuated accumulation of jasmonates modifies stomatal responses to water deficit
AU - De Ollas Valverde, Carlos Jose
AU - Arbona, Vicent
AU - Gomez-Cadenas, Aurelio
AU - Dodd, Ian Charles
PY - 2018/4/9
Y1 - 2018/4/9
N2 - To determine whether drought-induced root jasmonates (JA, jasmonic acid and JA-Ile, jasmonic acid iso-leucine) accumulation affected shoot responses to drying soil, near isogenic wild type (WT) tomato (Solanum lycopersicum cv. Castlemart) and the def-1 mutant (which fails to accumulate jasmonates during water deficit) were self- and reciprocally-grafted. Rootstock hydraulic conductance was entirely rootstock-dependent and significantly lower in def-1, yet def-1 scions maintained a higher leaf water potential as the soil dried due to their lower stomatal conductance (gs). Stomatal sensitivity to drying soil (the slope of gs versus soil water content) was low in def-1 self grafts but was normalised by grafting onto WT rootstocks. Although soil drying increased OPDA (a JA-precursor and putative antitranspirant) concentrations in def-1 scions, foliar JA accumulation was negligible and foliar ABA accumulation reduced compared to WT scions. A WT rootstock increased drought-induced ABA and JA accumulation in def-1 scions, but decreased OPDA accumulation.Xylem-borne jasmonates were biologically active, since supplying exogenous JA via the transpiration stream to detached leaves decreased transpiration of WT seedlings but had the opposite effect in def-1. Thus foliar accumulation of both ABA and JA at WT levels is required for both maximum (well watered) gs and stomatal sensitivity to drying soil.
AB - To determine whether drought-induced root jasmonates (JA, jasmonic acid and JA-Ile, jasmonic acid iso-leucine) accumulation affected shoot responses to drying soil, near isogenic wild type (WT) tomato (Solanum lycopersicum cv. Castlemart) and the def-1 mutant (which fails to accumulate jasmonates during water deficit) were self- and reciprocally-grafted. Rootstock hydraulic conductance was entirely rootstock-dependent and significantly lower in def-1, yet def-1 scions maintained a higher leaf water potential as the soil dried due to their lower stomatal conductance (gs). Stomatal sensitivity to drying soil (the slope of gs versus soil water content) was low in def-1 self grafts but was normalised by grafting onto WT rootstocks. Although soil drying increased OPDA (a JA-precursor and putative antitranspirant) concentrations in def-1 scions, foliar JA accumulation was negligible and foliar ABA accumulation reduced compared to WT scions. A WT rootstock increased drought-induced ABA and JA accumulation in def-1 scions, but decreased OPDA accumulation.Xylem-borne jasmonates were biologically active, since supplying exogenous JA via the transpiration stream to detached leaves decreased transpiration of WT seedlings but had the opposite effect in def-1. Thus foliar accumulation of both ABA and JA at WT levels is required for both maximum (well watered) gs and stomatal sensitivity to drying soil.
KW - Drought stress
KW - JA
KW - ABA
KW - grafting
KW - Solanum lycopersicum
U2 - 10.1093/jxb/ery045
DO - 10.1093/jxb/ery045
M3 - Journal article
VL - 69
SP - 2103
EP - 2116
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 8
ER -