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Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor. / Varzakakou, Maria; Roukas, Triantafyllos; Papaioannou, Emmanouil et al.
In: Preparative Biochemistry and Biotechnology, Vol. 41, No. 1, 01.2011, p. 7-21.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Varzakakou, M, Roukas, T, Papaioannou, E, Kotzekidou, P & Liakopoulou-Kyriakides, M 2011, 'Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor', Preparative Biochemistry and Biotechnology, vol. 41, no. 1, pp. 7-21. https://doi.org/10.1080/10826068.2010.525436

APA

Varzakakou, M., Roukas, T., Papaioannou, E., Kotzekidou, P., & Liakopoulou-Kyriakides, M. (2011). Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor. Preparative Biochemistry and Biotechnology, 41(1), 7-21. https://doi.org/10.1080/10826068.2010.525436

Vancouver

Varzakakou M, Roukas T, Papaioannou E, Kotzekidou P, Liakopoulou-Kyriakides M. Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor. Preparative Biochemistry and Biotechnology. 2011 Jan;41(1):7-21. Epub 2011 Jan 10. doi: 10.1080/10826068.2010.525436

Author

Varzakakou, Maria ; Roukas, Triantafyllos ; Papaioannou, Emmanouil et al. / Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor. In: Preparative Biochemistry and Biotechnology. 2011 ; Vol. 41, No. 1. pp. 7-21.

Bibtex

@article{f6fb2eeff6924d78aec14d63475f0b1a,
title = "Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor",
abstract = "The phenomenon of autolysis in Blakeslea trispora during carotene production from deproteinized hydrolyzed whey in an airlift reactor was investigated. The process of cellular autolysis was studied by measuring the changes in carotene concentration, dry biomass, residual sugars, pH, intracellular protein, specific activity of the hydrolytic enzymes (proteases, chitinase), and micromorphology of the fungus using a computerized image analysis system. All these parameters were useful indicators of autolysis, but image analysis was found to be the most useful indicator of the onset and progress of autolysis in the culture. Autolysis of B. trispora began early in the growth phase, continued during the stationary phase, and increased significantly in the decline phase. The morphological differentiation of the fungus was a result of the degradation of the cell membrane by hydrolytic enzymes. The biosynthesis of carotenes was carried out in the exponential phase, where the phenomenon of autolysis was not intense.",
keywords = "airlift reactor, autolysis, Blakeslea trispora, carotenes, deproteinized hydrolyzed whey, morphology",
author = "Maria Varzakakou and Triantafyllos Roukas and Emmanouil Papaioannou and Parthena Kotzekidou and Maria Liakopoulou-Kyriakides",
year = "2011",
month = jan,
doi = "10.1080/10826068.2010.525436",
language = "English",
volume = "41",
pages = "7--21",
journal = "Preparative Biochemistry and Biotechnology",
issn = "1082-6068",
publisher = "Taylor and Francis Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Autolysis of Blakeslea trispora during carotene production from cheese whey in an airlift reactor

AU - Varzakakou, Maria

AU - Roukas, Triantafyllos

AU - Papaioannou, Emmanouil

AU - Kotzekidou, Parthena

AU - Liakopoulou-Kyriakides, Maria

PY - 2011/1

Y1 - 2011/1

N2 - The phenomenon of autolysis in Blakeslea trispora during carotene production from deproteinized hydrolyzed whey in an airlift reactor was investigated. The process of cellular autolysis was studied by measuring the changes in carotene concentration, dry biomass, residual sugars, pH, intracellular protein, specific activity of the hydrolytic enzymes (proteases, chitinase), and micromorphology of the fungus using a computerized image analysis system. All these parameters were useful indicators of autolysis, but image analysis was found to be the most useful indicator of the onset and progress of autolysis in the culture. Autolysis of B. trispora began early in the growth phase, continued during the stationary phase, and increased significantly in the decline phase. The morphological differentiation of the fungus was a result of the degradation of the cell membrane by hydrolytic enzymes. The biosynthesis of carotenes was carried out in the exponential phase, where the phenomenon of autolysis was not intense.

AB - The phenomenon of autolysis in Blakeslea trispora during carotene production from deproteinized hydrolyzed whey in an airlift reactor was investigated. The process of cellular autolysis was studied by measuring the changes in carotene concentration, dry biomass, residual sugars, pH, intracellular protein, specific activity of the hydrolytic enzymes (proteases, chitinase), and micromorphology of the fungus using a computerized image analysis system. All these parameters were useful indicators of autolysis, but image analysis was found to be the most useful indicator of the onset and progress of autolysis in the culture. Autolysis of B. trispora began early in the growth phase, continued during the stationary phase, and increased significantly in the decline phase. The morphological differentiation of the fungus was a result of the degradation of the cell membrane by hydrolytic enzymes. The biosynthesis of carotenes was carried out in the exponential phase, where the phenomenon of autolysis was not intense.

KW - airlift reactor

KW - autolysis

KW - Blakeslea trispora

KW - carotenes

KW - deproteinized hydrolyzed whey

KW - morphology

U2 - 10.1080/10826068.2010.525436

DO - 10.1080/10826068.2010.525436

M3 - Journal article

C2 - 21229460

AN - SCOPUS:78651380468

VL - 41

SP - 7

EP - 21

JO - Preparative Biochemistry and Biotechnology

JF - Preparative Biochemistry and Biotechnology

SN - 1082-6068

IS - 1

ER -