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    Rights statement: http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 105 (2), pp 193-202 1992, © 1992 Cambridge University Press.

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Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms

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Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms. / Bates, P A; Robertson, C D; Tetley, L; Coombs, G H.

In: Parasitology, Vol. 105 , No. 2, 10.1992, p. 193-202.

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Bates, PA, Robertson, CD, Tetley, L & Coombs, GH 1992, 'Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms', Parasitology, vol. 105 , no. 2, pp. 193-202. https://doi.org/10.1017/S0031182000074102

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Bates, P A ; Robertson, C D ; Tetley, L ; Coombs, G H. / Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms. In: Parasitology. 1992 ; Vol. 105 , No. 2. pp. 193-202.

Bibtex

@article{e68958575ea3472b943aa86c3d294aa7,
title = "Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms",
abstract = "A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigote-like forms in Schneider's Drosophila medium supplemented with 20% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession of megasomes), biochemical (cysteine proteinase activity and gelatin SDS-PAGE banding pattern) and infectivity (in vivo) data are presented which show the close similarity of the cultured forms to lesion amastigotes. The axenically cultured forms grew optimally at a temperature of 32-33 degrees C, providing further evidence for their amastigote nature. It was found that adjustment of the pH of the growth medium to 5.4 was required in order to retain the amastigote morphology of the cultured parasites. This supports the notion that leishmanial amastigotes are acidophiles.",
keywords = "Leishmania mexicana, axenic culture , amastigotes , megasomes , cysteine proteinase , infectivity",
author = "Bates, {P A} and Robertson, {C D} and L Tetley and Coombs, {G H}",
note = "http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 105 (2), pp 193-202 1992, {\textcopyright} 1992 Cambridge University Press.",
year = "1992",
month = oct,
doi = "10.1017/S0031182000074102",
language = "English",
volume = "105 ",
pages = "193--202",
journal = "Parasitology",
issn = "0031-1820",
publisher = "Cambridge University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms

AU - Bates, P A

AU - Robertson, C D

AU - Tetley, L

AU - Coombs, G H

N1 - http://journals.cambridge.org/action/displayJournal?jid=PAR The final, definitive version of this article has been published in the Journal, Parasitology, 105 (2), pp 193-202 1992, © 1992 Cambridge University Press.

PY - 1992/10

Y1 - 1992/10

N2 - A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigote-like forms in Schneider's Drosophila medium supplemented with 20% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession of megasomes), biochemical (cysteine proteinase activity and gelatin SDS-PAGE banding pattern) and infectivity (in vivo) data are presented which show the close similarity of the cultured forms to lesion amastigotes. The axenically cultured forms grew optimally at a temperature of 32-33 degrees C, providing further evidence for their amastigote nature. It was found that adjustment of the pH of the growth medium to 5.4 was required in order to retain the amastigote morphology of the cultured parasites. This supports the notion that leishmanial amastigotes are acidophiles.

AB - A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigote-like forms in Schneider's Drosophila medium supplemented with 20% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession of megasomes), biochemical (cysteine proteinase activity and gelatin SDS-PAGE banding pattern) and infectivity (in vivo) data are presented which show the close similarity of the cultured forms to lesion amastigotes. The axenically cultured forms grew optimally at a temperature of 32-33 degrees C, providing further evidence for their amastigote nature. It was found that adjustment of the pH of the growth medium to 5.4 was required in order to retain the amastigote morphology of the cultured parasites. This supports the notion that leishmanial amastigotes are acidophiles.

KW - Leishmania mexicana

KW - axenic culture

KW - amastigotes

KW - megasomes

KW - cysteine proteinase

KW - infectivity

U2 - 10.1017/S0031182000074102

DO - 10.1017/S0031182000074102

M3 - Journal article

C2 - 1454417

VL - 105

SP - 193

EP - 202

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 2

ER -