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Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy

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Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy. / Bullock, Anthony J.; Garcia, Marcela; Shepherd, Joanna et al.

In: APPLIED SPECTROSCOPY REVIEWS, Vol. 55, No. 2, 28.02.2020, p. 158-171.

Research output: Contribution to Journal/MagazineReview articlepeer-review

Harvard

Bullock, AJ, Garcia, M, Shepherd, J, Rehman, I & MacNeil, S 2020, 'Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy', APPLIED SPECTROSCOPY REVIEWS, vol. 55, no. 2, pp. 158-171. https://doi.org/10.1080/05704928.2018.1558232

APA

Vancouver

Bullock AJ, Garcia M, Shepherd J, Rehman I, MacNeil S. Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy. APPLIED SPECTROSCOPY REVIEWS. 2020 Feb 28;55(2):158-171. Epub 2019 Jan 28. doi: 10.1080/05704928.2018.1558232

Author

Bullock, Anthony J. ; Garcia, Marcela ; Shepherd, Joanna et al. / Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy. In: APPLIED SPECTROSCOPY REVIEWS. 2020 ; Vol. 55, No. 2. pp. 158-171.

Bibtex

@article{05cf6574507149b99ed5eb1e35e807ed,
title = "Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy",
abstract = "Skin has a highly regulated pH environment of around pH 7.2 but with an acid barrier mantle of around pH 5.5. Trauma, inflammation, and infection are all thought to disrupt this pH environment but the lack of a non-invasive technique to measure pH within discrete locations within skin has hindered investigating what role pH plays in wound healing. In this study, a confocal Raman microspectroscopy method was used for measuring pH in a 3D tissue engineered model of human skin (TE-skin) and evaluated for its ability to detect changes in pH in response to wounding, inflammation and bacterial infection. The state of protonation of phosphate groups within the TE-skin was used to indicate pH in a non-destructive manner exploring depths of skin from the stratum corneum to 600 microns into the dermis. Deliberate wounding or inflammation (induced by IL-17) resulted in a loss of the acid mantle. Detailed scanning of TE-skin infected with Staphylococcus aureus or Pseudomonas aeruginosa revealed heterogeneous pH microenvironments ranging in size from 10 × 10 to 50 × 100 microns and ranging from pH 5 to 9. These microenvironments were not detected if an average pH for the TE-skin model was used.",
keywords = "pH, skin, bacteria, tissue-engineered",
author = "Bullock, {Anthony J.} and Marcela Garcia and Joanna Shepherd and Ihtesham Rehman and Sheila MacNeil",
year = "2020",
month = feb,
day = "28",
doi = "10.1080/05704928.2018.1558232",
language = "English",
volume = "55",
pages = "158--171",
journal = "APPLIED SPECTROSCOPY REVIEWS",
issn = "0570-4928",
publisher = "Taylor and Francis Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Bacteria induced pH changes in tissue-engineered human skin detected non-invasively using Raman confocal spectroscopy

AU - Bullock, Anthony J.

AU - Garcia, Marcela

AU - Shepherd, Joanna

AU - Rehman, Ihtesham

AU - MacNeil, Sheila

PY - 2020/2/28

Y1 - 2020/2/28

N2 - Skin has a highly regulated pH environment of around pH 7.2 but with an acid barrier mantle of around pH 5.5. Trauma, inflammation, and infection are all thought to disrupt this pH environment but the lack of a non-invasive technique to measure pH within discrete locations within skin has hindered investigating what role pH plays in wound healing. In this study, a confocal Raman microspectroscopy method was used for measuring pH in a 3D tissue engineered model of human skin (TE-skin) and evaluated for its ability to detect changes in pH in response to wounding, inflammation and bacterial infection. The state of protonation of phosphate groups within the TE-skin was used to indicate pH in a non-destructive manner exploring depths of skin from the stratum corneum to 600 microns into the dermis. Deliberate wounding or inflammation (induced by IL-17) resulted in a loss of the acid mantle. Detailed scanning of TE-skin infected with Staphylococcus aureus or Pseudomonas aeruginosa revealed heterogeneous pH microenvironments ranging in size from 10 × 10 to 50 × 100 microns and ranging from pH 5 to 9. These microenvironments were not detected if an average pH for the TE-skin model was used.

AB - Skin has a highly regulated pH environment of around pH 7.2 but with an acid barrier mantle of around pH 5.5. Trauma, inflammation, and infection are all thought to disrupt this pH environment but the lack of a non-invasive technique to measure pH within discrete locations within skin has hindered investigating what role pH plays in wound healing. In this study, a confocal Raman microspectroscopy method was used for measuring pH in a 3D tissue engineered model of human skin (TE-skin) and evaluated for its ability to detect changes in pH in response to wounding, inflammation and bacterial infection. The state of protonation of phosphate groups within the TE-skin was used to indicate pH in a non-destructive manner exploring depths of skin from the stratum corneum to 600 microns into the dermis. Deliberate wounding or inflammation (induced by IL-17) resulted in a loss of the acid mantle. Detailed scanning of TE-skin infected with Staphylococcus aureus or Pseudomonas aeruginosa revealed heterogeneous pH microenvironments ranging in size from 10 × 10 to 50 × 100 microns and ranging from pH 5 to 9. These microenvironments were not detected if an average pH for the TE-skin model was used.

KW - pH

KW - skin

KW - bacteria

KW - tissue-engineered

U2 - 10.1080/05704928.2018.1558232

DO - 10.1080/05704928.2018.1558232

M3 - Review article

VL - 55

SP - 158

EP - 171

JO - APPLIED SPECTROSCOPY REVIEWS

JF - APPLIED SPECTROSCOPY REVIEWS

SN - 0570-4928

IS - 2

ER -