Bacterial-dependent up-regulation of intestinal bile acid binding protein and transport is FXR-mediated following ileo-cecal resection

Biomedical and Life Sciences

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https://doi.org/10.1016/j.surg.2008.03.035
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Keywords

NUCLEAR RECEPTOR, GROWTH, ALPHA, LIVER, BETA, MICE, GUT

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Bacterial-dependent up-regulation of intestinal bile acid binding protein and transport is FXR-mediated following ileo-cecal resection. / Dekaney, Christopher M.; von Allmen, Douglas C.; Garrison, Aaron P. et al.
In: Surgery, Vol. 144, No. 2, 08.2008, p. 174-181.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{6e1dfddaae054fa6abb9d2b726586809,

title = "Bacterial-dependent up-regulation of intestinal bile acid binding protein and transport is FXR-mediated following ileo-cecal resection",

abstract = "Background. Bile acid (BA) reclamation following ileo-cecal resection (ICR) may prevent colonic mucosa from chronic injury. In this study, we hypothesized that in a marine model of ICR the remnant colon would upregulate the cellular machinery necessary for BA reclamation and would do so in an FXR- and bacteria-dependent manner.Methods. Conventional (WT), conventional FXR knockout (FXR null) and germ free (GF) mice were randomized to undergo either ICR or sham operation. The ascending colon was harvested for histology and immunohistochemistry and changes in bile acid homeostatic gene expression determined by real-tinge polymerase chain reaction (RTPCR) 7 days following surgery.Results. Following ICR WT mice showed significant increases in the expression of genes regulating bile acid transport including IBABP, Asbt, Ost beta and FGF 15. Increased expression of IBABP and Asbt was confirmed by immunohistochemistry. Induction of bile acid transport genes was absent or attenuated in FXR null and GF mice.Conclusion. Bacterial dependent up regulation of IBABP is FXR mediated in the colon following ICR Mice lacking microbiota (GF) or FXR are unable to increase the expression of IBABP or FGF 15.",

keywords = "NUCLEAR RECEPTOR, GROWTH, ALPHA, LIVER, BETA, MICE, GUT",

author = "Dekaney, {Christopher M.} and {von Allmen}, {Douglas C.} and Garrison, {Aaron P.} and Rigby, {Rachael J.} and Lund, {P. Kay} and Henning, {Susan J.} and Helmrath, {Michael A.}",

year = "2008",

month = aug,

doi = "10.1016/j.surg.2008.03.035",

language = "English",

volume = "144",

pages = "174--181",

journal = "Surgery",

issn = "0039-6060",

publisher = "Mosby Inc.",

number = "2",

}

RIS

TY - JOUR

T1 - Bacterial-dependent up-regulation of intestinal bile acid binding protein and transport is FXR-mediated following ileo-cecal resection

AU - Dekaney, Christopher M.

AU - von Allmen, Douglas C.

AU - Garrison, Aaron P.

AU - Rigby, Rachael J.

AU - Lund, P. Kay

AU - Henning, Susan J.

AU - Helmrath, Michael A.

PY - 2008/8

Y1 - 2008/8

N2 - Background. Bile acid (BA) reclamation following ileo-cecal resection (ICR) may prevent colonic mucosa from chronic injury. In this study, we hypothesized that in a marine model of ICR the remnant colon would upregulate the cellular machinery necessary for BA reclamation and would do so in an FXR- and bacteria-dependent manner.Methods. Conventional (WT), conventional FXR knockout (FXR null) and germ free (GF) mice were randomized to undergo either ICR or sham operation. The ascending colon was harvested for histology and immunohistochemistry and changes in bile acid homeostatic gene expression determined by real-tinge polymerase chain reaction (RTPCR) 7 days following surgery.Results. Following ICR WT mice showed significant increases in the expression of genes regulating bile acid transport including IBABP, Asbt, Ost beta and FGF 15. Increased expression of IBABP and Asbt was confirmed by immunohistochemistry. Induction of bile acid transport genes was absent or attenuated in FXR null and GF mice.Conclusion. Bacterial dependent up regulation of IBABP is FXR mediated in the colon following ICR Mice lacking microbiota (GF) or FXR are unable to increase the expression of IBABP or FGF 15.

AB - Background. Bile acid (BA) reclamation following ileo-cecal resection (ICR) may prevent colonic mucosa from chronic injury. In this study, we hypothesized that in a marine model of ICR the remnant colon would upregulate the cellular machinery necessary for BA reclamation and would do so in an FXR- and bacteria-dependent manner.Methods. Conventional (WT), conventional FXR knockout (FXR null) and germ free (GF) mice were randomized to undergo either ICR or sham operation. The ascending colon was harvested for histology and immunohistochemistry and changes in bile acid homeostatic gene expression determined by real-tinge polymerase chain reaction (RTPCR) 7 days following surgery.Results. Following ICR WT mice showed significant increases in the expression of genes regulating bile acid transport including IBABP, Asbt, Ost beta and FGF 15. Increased expression of IBABP and Asbt was confirmed by immunohistochemistry. Induction of bile acid transport genes was absent or attenuated in FXR null and GF mice.Conclusion. Bacterial dependent up regulation of IBABP is FXR mediated in the colon following ICR Mice lacking microbiota (GF) or FXR are unable to increase the expression of IBABP or FGF 15.

KW - NUCLEAR RECEPTOR

KW - GROWTH

KW - ALPHA

KW - LIVER

KW - BETA

KW - MICE

KW - GUT

UR - http://www.scopus.com/inward/record.url?scp=47549117542&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2008.03.035

DO - 10.1016/j.surg.2008.03.035

M3 - Journal article

VL - 144

SP - 174

EP - 181

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 2

ER -

Research

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