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Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α

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Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α. / Pourvali, K.; Matak, P.; Latunde-Dada, G.O. et al.
In: FEBS Letters, Vol. 586, No. 16, 30.07.2012, p. 2423-2427.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Pourvali, K, Matak, P, Latunde-Dada, GO, Solomou, S, Mastrogiannaki, M, Peyssonnaux, C & Sharp, PA 2012, 'Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α', FEBS Letters, vol. 586, no. 16, pp. 2423-2427. https://doi.org/10.1016/j.febslet.2012.05.058

APA

Pourvali, K., Matak, P., Latunde-Dada, G. O., Solomou, S., Mastrogiannaki, M., Peyssonnaux, C., & Sharp, P. A. (2012). Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α. FEBS Letters, 586(16), 2423-2427. https://doi.org/10.1016/j.febslet.2012.05.058

Vancouver

Pourvali K, Matak P, Latunde-Dada GO, Solomou S, Mastrogiannaki M, Peyssonnaux C et al. Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α. FEBS Letters. 2012 Jul 30;586(16):2423-2427. doi: 10.1016/j.febslet.2012.05.058

Author

Pourvali, K. ; Matak, P. ; Latunde-Dada, G.O. et al. / Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α. In: FEBS Letters. 2012 ; Vol. 586, No. 16. pp. 2423-2427.

Bibtex

@article{4141a6c527fb4840b5ae0ffcb407368b,
title = "Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α",
abstract = "Hypoxia, via stabilization of HIF2α, regulates the expression of the intestinal iron transporters DMT1 and ferroportin. Here we investigated whether the intestinal copper importer Ctr1 was also regulated by hypoxia. Copper uptake and Ctr1 mRNA expression were significantly increased in Caco‐2 cells exposed to hypoxia. To determine whether HIF2α was involved in regulation of Ctr1 expression, we employed three models of HIF2α knockdown (chemical suppression of HIF2α translation in Caco‐2 cells; HIF2α‐siRNA‐treated HuTu80 cells; HIF2α‐intestinal knockout mice); Ctr1 mRNA expression was decreased in all three models under normoxic conditions. HIF2α translational inhibitor did not alter Ctr1 expression under hypoxic conditions. We conclude that basal expression of Ctr1 is regulated by HIF2α; however, the induction by hypoxia is a HIF2α‐independent event.",
author = "K. Pourvali and P. Matak and G.O. Latunde-Dada and S. Solomou and M. Mastrogiannaki and C. Peyssonnaux and P.A. Sharp",
year = "2012",
month = jul,
day = "30",
doi = "10.1016/j.febslet.2012.05.058",
language = "English",
volume = "586",
pages = "2423--2427",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "16",

}

RIS

TY - JOUR

T1 - Basal expression of copper transporter 1 in intestinal epithelial cells is regulated by hypoxia-inducible factor 2α

AU - Pourvali, K.

AU - Matak, P.

AU - Latunde-Dada, G.O.

AU - Solomou, S.

AU - Mastrogiannaki, M.

AU - Peyssonnaux, C.

AU - Sharp, P.A.

PY - 2012/7/30

Y1 - 2012/7/30

N2 - Hypoxia, via stabilization of HIF2α, regulates the expression of the intestinal iron transporters DMT1 and ferroportin. Here we investigated whether the intestinal copper importer Ctr1 was also regulated by hypoxia. Copper uptake and Ctr1 mRNA expression were significantly increased in Caco‐2 cells exposed to hypoxia. To determine whether HIF2α was involved in regulation of Ctr1 expression, we employed three models of HIF2α knockdown (chemical suppression of HIF2α translation in Caco‐2 cells; HIF2α‐siRNA‐treated HuTu80 cells; HIF2α‐intestinal knockout mice); Ctr1 mRNA expression was decreased in all three models under normoxic conditions. HIF2α translational inhibitor did not alter Ctr1 expression under hypoxic conditions. We conclude that basal expression of Ctr1 is regulated by HIF2α; however, the induction by hypoxia is a HIF2α‐independent event.

AB - Hypoxia, via stabilization of HIF2α, regulates the expression of the intestinal iron transporters DMT1 and ferroportin. Here we investigated whether the intestinal copper importer Ctr1 was also regulated by hypoxia. Copper uptake and Ctr1 mRNA expression were significantly increased in Caco‐2 cells exposed to hypoxia. To determine whether HIF2α was involved in regulation of Ctr1 expression, we employed three models of HIF2α knockdown (chemical suppression of HIF2α translation in Caco‐2 cells; HIF2α‐siRNA‐treated HuTu80 cells; HIF2α‐intestinal knockout mice); Ctr1 mRNA expression was decreased in all three models under normoxic conditions. HIF2α translational inhibitor did not alter Ctr1 expression under hypoxic conditions. We conclude that basal expression of Ctr1 is regulated by HIF2α; however, the induction by hypoxia is a HIF2α‐independent event.

U2 - 10.1016/j.febslet.2012.05.058

DO - 10.1016/j.febslet.2012.05.058

M3 - Journal article

VL - 586

SP - 2423

EP - 2427

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 16

ER -