Behaviour of two IgG subclasses in transport of immunoglobulin across the human placenta

Keywords

placenta, immunoglobulin, chorionic villi, syncytiotrophoblast, IgG subclasses, GAMMA-RECEPTOR-II, COATED VESICLES, FC-RECEPTORS

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Behaviour of two IgG subclasses in transport of immunoglobulin across the human placenta. / Mongan, L C ; Ockleford, C D .
In: Journal of Anatomy, Vol. 188, 02.1996, p. 43-51.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{234a92710da04cfc83a2b49889625c91,

title = "Behaviour of two IgG subclasses in transport of immunoglobulin across the human placenta",

abstract = "The human IgG subclasses are a family of highly related yet distinct molecules. Each of these four subclasses performs a discrete function within the human immune system. Previous studies have shown that one of these molecules, hIgG2, may be discriminated against in transport across the human placenta. We have aimed to elucidate the mechanism of this discrimination in order to gain a more comprehensive understanding of the process of transport of immunoglobulin across the human placenta. We have used a combination of immunocytochemical localisation and biochemical analysis to detail the behaviour of hIgG2. Confocal laser scanning microscopy was used to compare the localisation of hIgG1 (chosen as representative of the efficiently transported subclasses) and hIgG2 in term and first trimester chorionic villi.Complementary evidence was provided from immunoblot analysis of isolated placental coated vesicles. The data presented here suggest that hIgG2 is transported into the syncytiotrophoblast and appears to accumulate in the stroma of the villi. This leads us to the hypothesis that the fetal capillary endothelium is the cellular impediment to the transport of hIgG2 into the fetal circulation.",

keywords = "placenta, immunoglobulin, chorionic villi, syncytiotrophoblast, IgG subclasses, GAMMA-RECEPTOR-II, COATED VESICLES, FC-RECEPTORS",

author = "Mongan, {L C} and Ockleford, {C D}",

year = "1996",

month = feb,

language = "English",

volume = "188",

pages = "43--51",

journal = "Journal of Anatomy",

issn = "0021-8782",

publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Behaviour of two IgG subclasses in transport of immunoglobulin across the human placenta

AU - Mongan, L C

AU - Ockleford, C D

PY - 1996/2

Y1 - 1996/2

N2 - The human IgG subclasses are a family of highly related yet distinct molecules. Each of these four subclasses performs a discrete function within the human immune system. Previous studies have shown that one of these molecules, hIgG2, may be discriminated against in transport across the human placenta. We have aimed to elucidate the mechanism of this discrimination in order to gain a more comprehensive understanding of the process of transport of immunoglobulin across the human placenta. We have used a combination of immunocytochemical localisation and biochemical analysis to detail the behaviour of hIgG2. Confocal laser scanning microscopy was used to compare the localisation of hIgG1 (chosen as representative of the efficiently transported subclasses) and hIgG2 in term and first trimester chorionic villi.Complementary evidence was provided from immunoblot analysis of isolated placental coated vesicles. The data presented here suggest that hIgG2 is transported into the syncytiotrophoblast and appears to accumulate in the stroma of the villi. This leads us to the hypothesis that the fetal capillary endothelium is the cellular impediment to the transport of hIgG2 into the fetal circulation.

AB - The human IgG subclasses are a family of highly related yet distinct molecules. Each of these four subclasses performs a discrete function within the human immune system. Previous studies have shown that one of these molecules, hIgG2, may be discriminated against in transport across the human placenta. We have aimed to elucidate the mechanism of this discrimination in order to gain a more comprehensive understanding of the process of transport of immunoglobulin across the human placenta. We have used a combination of immunocytochemical localisation and biochemical analysis to detail the behaviour of hIgG2. Confocal laser scanning microscopy was used to compare the localisation of hIgG1 (chosen as representative of the efficiently transported subclasses) and hIgG2 in term and first trimester chorionic villi.Complementary evidence was provided from immunoblot analysis of isolated placental coated vesicles. The data presented here suggest that hIgG2 is transported into the syncytiotrophoblast and appears to accumulate in the stroma of the villi. This leads us to the hypothesis that the fetal capillary endothelium is the cellular impediment to the transport of hIgG2 into the fetal circulation.

KW - placenta

KW - immunoglobulin

KW - chorionic villi

KW - syncytiotrophoblast

KW - IgG subclasses

KW - GAMMA-RECEPTOR-II

KW - COATED VESICLES

KW - FC-RECEPTORS

M3 - Journal article

VL - 188

SP - 43

EP - 51

JO - Journal of Anatomy

JF - Journal of Anatomy

SN - 0021-8782

ER -

Research

Keywords