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Bidirectional isotachophoresis on a planar chip with integrated conductivity detection

Research output: Contribution to journalJournal articlepeer-review

Published
<mark>Journal publication date</mark>9/10/2002
<mark>Journal</mark>Analyst
Issue number11
Volume127
Number of pages7
Pages (from-to)1413-1419
Publication StatusPublished
<mark>Original language</mark>English

Abstract

The use of a miniaturised planar separation device with integrated conductivity detection for performing bidirectional isotachophoresis (ITP) is described. The chips were produced in poly(methyl methacrylate) (PMMA) using a milling procedure. To enable bidirectional ITP the devices were designed to inject samples into the centre of the separation channel and incorporated two integrated on-column conductivity detectors, positioned at opposite ends of this channel. When used with a hydrodynamic sample transport system the devices were used for the analysis of a range of small ions: NH4+; Na+; Mg2+; Ca2+; Li+; NO3−; ClO4−; SO42−; F−. Results sucessfully achieved included the simultaneous separation of three anions and three cations.