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Rights statement: Open access
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Rights statement: © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
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Rights statement: © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering
AU - Hardy, John George
AU - Torres-Rendon, Jose Guillermo
AU - Leal-Egana, Aldo
AU - Walther, Andreas
AU - Schlaad, Helmut
AU - Cölfen, Helmut
AU - Scheibel, Thomas Rainer
PY - 2016/7/11
Y1 - 2016/7/11
N2 - Materials based on biodegradable polyesters such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT) have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein is reported the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
AB - Materials based on biodegradable polyesters such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT) have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein is reported the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
KW - spider silk
KW - recombinant protein
KW - biodegradable polymers
KW - biomaterials
KW - biomineralization
KW - bone tissue engineering
U2 - 10.3390/ma9070560
DO - 10.3390/ma9070560
M3 - Journal article
VL - 9
JO - Materials
JF - Materials
SN - 1996-1944
IS - 7
M1 - 560
ER -