Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Blood meal identification and parasite detection in laboratory-fed and field-captured Lutzomyia longipalpis by PCR using FTA databasing paper
AU - Sant'Anna, Mauricio R V
AU - Jones, Nathaniel G
AU - Hindley, Jonathan A
AU - Mendes-Sousa, Antonio F
AU - Dillon, Rod J
AU - Cavalcante, Reginaldo R
AU - Alexander, Bruce
AU - Bates, Paul A
PY - 2008
Y1 - 2008
N2 - The phlebotomine sand fly Lutzomyia longipalpis takes blood from a variety of wild and domestic animals and transmits Leishmania (Leishmania) infantum chagasi, etiological agent of American visceral leishmaniasis. Blood meal identification in sand flies has depended largely on serological methods but a new protocol described here uses filter-based technology to stabilise and store blood meal DNA, allowing subsequent PCR identification of blood meal sources, as well as parasite detection, in blood-fed sand flies. This technique revealed that 53.6% of field-collected sand flies captured in the back yards of houses in Teresina (Brazil) had fed on chickens. The potential applications of this technique in epidemiological studies and strategic planning for leishmaniasis control programmes are discussed.
AB - The phlebotomine sand fly Lutzomyia longipalpis takes blood from a variety of wild and domestic animals and transmits Leishmania (Leishmania) infantum chagasi, etiological agent of American visceral leishmaniasis. Blood meal identification in sand flies has depended largely on serological methods but a new protocol described here uses filter-based technology to stabilise and store blood meal DNA, allowing subsequent PCR identification of blood meal sources, as well as parasite detection, in blood-fed sand flies. This technique revealed that 53.6% of field-collected sand flies captured in the back yards of houses in Teresina (Brazil) had fed on chickens. The potential applications of this technique in epidemiological studies and strategic planning for leishmaniasis control programmes are discussed.
KW - Animals
KW - Animals, Domestic
KW - Animals, Wild
KW - Blood
KW - Brazil
KW - Cricetinae
KW - Feeding Behavior
KW - Female
KW - Humans
KW - Leishmaniasis, Visceral
KW - Parasitology
KW - Polymerase Chain Reaction
KW - Psychodidae
KW - Specimen Handling
U2 - 10.1016/j.actatropica.2008.06.003
DO - 10.1016/j.actatropica.2008.06.003
M3 - Journal article
C2 - 18606150
VL - 107
SP - 230
EP - 237
JO - Acta Tropica
JF - Acta Tropica
IS - 3
ER -