Final published version
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Caloric restriction improves basal redox parameters in hippocampus and cerebral cortex of Wistar rats
AU - Ribeiro, Leticia C
AU - Rodrigues, Letícia
AU - Quincozes-Santos, André
AU - Tramontina, Ana C
AU - Bambini-Junior, Victorio
AU - Zanotto, Caroline
AU - Diehl, Luisa A
AU - Biasibetti, Regina
AU - Kleinkauf-Rocha, Juliana
AU - Dalmaz, Carla
AU - Goncalves, Carlos-Alberto
AU - Gottfried, Carmem
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2012/9/7
Y1 - 2012/9/7
N2 - Caloric restriction (CR) has been shown to either decrease or prevent the progression of several age-related pathologies. In previous work, we demonstrated that CR modulates astrocyte functions, suggesting that CR may exert neuroglial modulation. Here, we investigated the effects of CR on hippocampal (Hc) and cortical (Cx) oxidative stress parameters of male Wistar rats. Our results showed that CR-fed rats had 17% less body weight gain after 12 weeks of treatment. CR improved locomotion performance, increased glutathione levels and decreased glutathione peroxidase activity and the production of reactive oxygen species. However, no changes were observed in lipid peroxidation, nitric oxide content and catalase activity. Single cell gel electrophoresis assay (comet assay) revealed a reduction in the extent of basal DNA damage upon CR. Our data suggest that dietary CR could induce both hippocampal and cortical modulation resulting in metabolic changes and as a consequence, significant improvement of cellular defense-associated parameters.
AB - Caloric restriction (CR) has been shown to either decrease or prevent the progression of several age-related pathologies. In previous work, we demonstrated that CR modulates astrocyte functions, suggesting that CR may exert neuroglial modulation. Here, we investigated the effects of CR on hippocampal (Hc) and cortical (Cx) oxidative stress parameters of male Wistar rats. Our results showed that CR-fed rats had 17% less body weight gain after 12 weeks of treatment. CR improved locomotion performance, increased glutathione levels and decreased glutathione peroxidase activity and the production of reactive oxygen species. However, no changes were observed in lipid peroxidation, nitric oxide content and catalase activity. Single cell gel electrophoresis assay (comet assay) revealed a reduction in the extent of basal DNA damage upon CR. Our data suggest that dietary CR could induce both hippocampal and cortical modulation resulting in metabolic changes and as a consequence, significant improvement of cellular defense-associated parameters.
KW - Animals
KW - Behavior, Animal
KW - Caloric Restriction
KW - Cerebral Cortex/metabolism
KW - Comet Assay
KW - DNA Damage
KW - Male
KW - Motor Activity/physiology
KW - Oxidation-Reduction
KW - Rats
KW - Rats, Wistar
KW - Reactive Oxygen Species/metabolism
U2 - 10.1016/j.brainres.2012.07.021
DO - 10.1016/j.brainres.2012.07.021
M3 - Journal article
C2 - 22842081
VL - 1472
SP - 11
EP - 19
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -