Rights statement: This is an author-created, un-copyedited version of an article accepted for publication/published in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi:10.1088/1748-605X/aa6200
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Ca:Mg:Zn:CO3 and Ca:Mg:CO3-tri- and bi-elemental carbonate microparticles for novel injectable self-gelling hydrogel-microparticle composites for tissue regeneration
AU - Douglas, Timothy Edward Lim
AU - Sobczyk, Katarzyna
AU - Łapa, Agata
AU - Włodarczyk, Katarzyna
AU - Brackman, Gilles
AU - Vidiasheva, Irina
AU - Reczyńska, Katarzyna
AU - Pietryga, Krzysztof
AU - Schaubroeck, David
AU - Bliznuk, Vitaliy
AU - Van Der Voort, Pascal
AU - Declercq, Heidi
AU - Van den Bulcke, Jan
AU - Samal, Sangram Keshari
AU - Khalenkow, Dmitry
AU - Parakhonskiy, Bogdan
AU - Van Acker, Joris
AU - Coenye, Tom
AU - Lewandowska-Szumieł, Małgorzata
AU - Pamuła, Elżbieta
AU - Skirtach, Andre
N1 - This is an author-created, un-copyedited version of an article accepted for publication/published in Biomedical Materials. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at doi:10.1088/1748-605X/aa6200
PY - 2017/3/24
Y1 - 2017/3/24
N2 - Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg. Zn and Ca were incorporated into microparticle preparations to a greater extent than Mg. Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition. Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites. Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus. Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial. This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
AB - Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum (GG), an anionic polysaccharide, to form injectable hydrogel-microparticle composites, containing Zn, Ca and Mg. Zn and Ca were incorporated into microparticle preparations to a greater extent than Mg. Microparticle groups were heterogeneous and contained microparticles of differing shape and elemental composition. Zn-rich microparticles were 'star shaped' and appeared to consist of small crystallites, while Zn-poor, Ca- and Mg-rich microparticles were irregular in shape and appeared to contain lager crystallites. Zn-free microparticle groups exhibited the best cytocompatibility and, unexpectedly, Zn-free composites showed the highest antibacterial activity towards methicilin-resistant Staphylococcus aureus. Composites containing Zn-free microparticles were cytocompatible and therefore appear most suitable for applications as an injectable biomaterial. This study proves the principle of creating bi- and tri-elemental microparticles to induce the gelation of GG to create injectable hydrogel-microparticle composites.
KW - hydrogel
KW - composite
KW - injectable
KW - zinc
KW - magnesium
KW - gellan gum
KW - carbonate
U2 - 10.1088/1748-605X/aa6200
DO - 10.1088/1748-605X/aa6200
M3 - Journal article
VL - 12
JO - Biomedical Materials
JF - Biomedical Materials
SN - 1748-6041
IS - 2
M1 - 025015
ER -