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Candida albicans as an essential “keystone” component within polymicrobial oral biofilm models?

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Candida albicans as an essential “keystone” component within polymicrobial oral biofilm models? / Young, T.; Alshanta, O.-A.; Kean, R.; Bradshaw, D.; Pratten, J.; Williams, C.; Woodall, C.; Ramage, G.; Brown, J.L.

In: Microorganisms , Vol. 9, No. 1, 59, 28.12.2020.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Young, T, Alshanta, O-A, Kean, R, Bradshaw, D, Pratten, J, Williams, C, Woodall, C, Ramage, G & Brown, JL 2020, 'Candida albicans as an essential “keystone” component within polymicrobial oral biofilm models?', Microorganisms , vol. 9, no. 1, 59. https://doi.org/10.3390/microorganisms9010059

APA

Young, T., Alshanta, O-A., Kean, R., Bradshaw, D., Pratten, J., Williams, C., Woodall, C., Ramage, G., & Brown, J. L. (2020). Candida albicans as an essential “keystone” component within polymicrobial oral biofilm models? Microorganisms , 9(1), [59]. https://doi.org/10.3390/microorganisms9010059

Vancouver

Author

Young, T. ; Alshanta, O.-A. ; Kean, R. ; Bradshaw, D. ; Pratten, J. ; Williams, C. ; Woodall, C. ; Ramage, G. ; Brown, J.L. / Candida albicans as an essential “keystone” component within polymicrobial oral biofilm models?. In: Microorganisms . 2020 ; Vol. 9, No. 1.

Bibtex

@article{bc88db9acc3048a7ae9989dd84cd2321,
title = "Candida albicans as an essential “keystone” component within polymicrobial oral biofilm models?",
abstract = "Background: Existing standardized biofilm assays focus on simple mono-species or bacterial-only models. Incorporating Candida albicans into complex biofilm models can offer a more appropriate and relevant polymicrobial biofilm for the development of oral health products. Aims: This study aimed to assess the importance of interkingdom interactions in polymicrobial oral biofilm systems with or without C. albicans, and test how these models respond to oral therapeutic challenges in vitro. Materials and Methods: Polymicrobial biofilms (two models containing 5 and 10 bacterial species, respectively) were created in parallel in the presence and absence of C. albicans and challenged using clinically relevant antimicrobials. The metabolic profiles and biomasses of these complex biofilms were estimated using resazurin dye and crystal violet stain, respectively. Quantitative PCR was utilized to assess compositional changes in microbial load. Additional assays, for measurements of pH and lactate, were included to monitor fluctuations in virulence “biomarkers.” Results: An increased level of metabolic activity and biomass in the presence of C. albicans was observed. Bacterial load was increased by more than a factor of 10 in the presence of C. albicans. Assays showed inclusion of C. albicans impacted the biofilm virulence profiles. C. albicans did not affect the biofilms{\textquoteright} responses to the short-term incubations with different treatments. Conclusions: The interkingdom biofilms described herein are structurally robust and exhibit all the hallmarks of a reproducible model. To our knowledge, these data are the first to test the hypothesis that yeasts may act as potential “keystone” components of oral biofilms. {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",
keywords = "Antimicro-bials, Biofilm models, Fungal–bacterial interactions, High-throughput, Oral biofilm, Polymicrobial",
author = "T. Young and O.-A. Alshanta and R. Kean and D. Bradshaw and J. Pratten and C. Williams and C. Woodall and G. Ramage and J.L. Brown",
year = "2020",
month = dec,
day = "28",
doi = "10.3390/microorganisms9010059",
language = "English",
volume = "9",
journal = "Microorganisms ",
issn = "2076-2607",
publisher = "MDPI - Open Access Publishing",
number = "1",

}

RIS

TY - JOUR

T1 - Candida albicans as an essential “keystone” component within polymicrobial oral biofilm models?

AU - Young, T.

AU - Alshanta, O.-A.

AU - Kean, R.

AU - Bradshaw, D.

AU - Pratten, J.

AU - Williams, C.

AU - Woodall, C.

AU - Ramage, G.

AU - Brown, J.L.

PY - 2020/12/28

Y1 - 2020/12/28

N2 - Background: Existing standardized biofilm assays focus on simple mono-species or bacterial-only models. Incorporating Candida albicans into complex biofilm models can offer a more appropriate and relevant polymicrobial biofilm for the development of oral health products. Aims: This study aimed to assess the importance of interkingdom interactions in polymicrobial oral biofilm systems with or without C. albicans, and test how these models respond to oral therapeutic challenges in vitro. Materials and Methods: Polymicrobial biofilms (two models containing 5 and 10 bacterial species, respectively) were created in parallel in the presence and absence of C. albicans and challenged using clinically relevant antimicrobials. The metabolic profiles and biomasses of these complex biofilms were estimated using resazurin dye and crystal violet stain, respectively. Quantitative PCR was utilized to assess compositional changes in microbial load. Additional assays, for measurements of pH and lactate, were included to monitor fluctuations in virulence “biomarkers.” Results: An increased level of metabolic activity and biomass in the presence of C. albicans was observed. Bacterial load was increased by more than a factor of 10 in the presence of C. albicans. Assays showed inclusion of C. albicans impacted the biofilm virulence profiles. C. albicans did not affect the biofilms’ responses to the short-term incubations with different treatments. Conclusions: The interkingdom biofilms described herein are structurally robust and exhibit all the hallmarks of a reproducible model. To our knowledge, these data are the first to test the hypothesis that yeasts may act as potential “keystone” components of oral biofilms. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

AB - Background: Existing standardized biofilm assays focus on simple mono-species or bacterial-only models. Incorporating Candida albicans into complex biofilm models can offer a more appropriate and relevant polymicrobial biofilm for the development of oral health products. Aims: This study aimed to assess the importance of interkingdom interactions in polymicrobial oral biofilm systems with or without C. albicans, and test how these models respond to oral therapeutic challenges in vitro. Materials and Methods: Polymicrobial biofilms (two models containing 5 and 10 bacterial species, respectively) were created in parallel in the presence and absence of C. albicans and challenged using clinically relevant antimicrobials. The metabolic profiles and biomasses of these complex biofilms were estimated using resazurin dye and crystal violet stain, respectively. Quantitative PCR was utilized to assess compositional changes in microbial load. Additional assays, for measurements of pH and lactate, were included to monitor fluctuations in virulence “biomarkers.” Results: An increased level of metabolic activity and biomass in the presence of C. albicans was observed. Bacterial load was increased by more than a factor of 10 in the presence of C. albicans. Assays showed inclusion of C. albicans impacted the biofilm virulence profiles. C. albicans did not affect the biofilms’ responses to the short-term incubations with different treatments. Conclusions: The interkingdom biofilms described herein are structurally robust and exhibit all the hallmarks of a reproducible model. To our knowledge, these data are the first to test the hypothesis that yeasts may act as potential “keystone” components of oral biofilms. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

KW - Antimicro-bials

KW - Biofilm models

KW - Fungal–bacterial interactions

KW - High-throughput

KW - Oral biofilm

KW - Polymicrobial

U2 - 10.3390/microorganisms9010059

DO - 10.3390/microorganisms9010059

M3 - Journal article

VL - 9

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 1

M1 - 59

ER -