Home > Research > Publications & Outputs > Carbapenemase-producing Enterobacteriaceae in h...

Electronic data

  • Author Accepted Manuscript

    Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Hospital Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hospital Infection, 93, 2, 2016 DOI: 10.1016/j.jhin.2016.03.007

    Accepted author manuscript, 359 KB, PDF document

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Links

Text available via DOI:

View graph of relations

Carbapenemase-producing Enterobacteriaceae in hospital wastewater: a reservoir that may be unrelated to clinical isolates [star]

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Carbapenemase-producing Enterobacteriaceae in hospital wastewater : a reservoir that may be unrelated to clinical isolates [star]. / White, Leila; Hopkins, Katie ; Meunier, Danièle et al.

In: Journal of Hospital Infection, Vol. 93, No. 2, 06.2016, p. 145-151.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

White, L, Hopkins, K, Meunier, D, Perry, CL, Pike, R, Wilkinson, P, Pickup, RW, Cheesbrough, J & Woodford, N 2016, 'Carbapenemase-producing Enterobacteriaceae in hospital wastewater: a reservoir that may be unrelated to clinical isolates [star]', Journal of Hospital Infection, vol. 93, no. 2, pp. 145-151. https://doi.org/10.1016/j.jhin.2016.03.007

APA

White, L., Hopkins, K., Meunier, D., Perry, C. L., Pike, R., Wilkinson, P., Pickup, R. W., Cheesbrough, J., & Woodford, N. (2016). Carbapenemase-producing Enterobacteriaceae in hospital wastewater: a reservoir that may be unrelated to clinical isolates [star]. Journal of Hospital Infection, 93(2), 145-151. https://doi.org/10.1016/j.jhin.2016.03.007

Vancouver

White L, Hopkins K, Meunier D, Perry CL, Pike R, Wilkinson P et al. Carbapenemase-producing Enterobacteriaceae in hospital wastewater: a reservoir that may be unrelated to clinical isolates [star]. Journal of Hospital Infection. 2016 Jun;93(2):145-151. Epub 2016 Mar 24. doi: 10.1016/j.jhin.2016.03.007

Author

White, Leila ; Hopkins, Katie ; Meunier, Danièle et al. / Carbapenemase-producing Enterobacteriaceae in hospital wastewater : a reservoir that may be unrelated to clinical isolates [star]. In: Journal of Hospital Infection. 2016 ; Vol. 93, No. 2. pp. 145-151.

Bibtex

@article{e7580e10b0dd45b6a4ff2f3d33c4966d,
title = "Carbapenemase-producing Enterobacteriaceae in hospital wastewater: a reservoir that may be unrelated to clinical isolates [star]",
abstract = "SummaryBackground: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging infection control problem in hospitals worldwide. Identifying carriers can help reduce potential spread and infections.Aim: To assess whether testing hospital wastewater for CPE can supplement patient based screening for infection prevention purposes in a hospital without a recognised endemic CPE problem.Methods: Wastewater collected from hospital pipework on 16 occasions during February-March 2014 was screened for CPE using chromID{\textregistered}CARBA agar and chromID{\textregistered}CPS agar with a 10 μg ertapenem disc and combination disc testing. MICs were determined using British Society for Antimicrobial Chemotherapy methodology and carbapenemase genes detected by PCR or wholegenomesequencing. Selected isolates were typed by PFGE.Findings: Suspected CPE were recovered from all 16 wastewater samples. Of 17 isolates sent to Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, six (four Citrobacter freundii and two Enterobacter cloacae complex) were New Delhi metallo–beta-lactamase (NDM) producers and the remaining 11 (six Klebsiella oxytoca and five Enterobacter cloacae complex),Guiana-Extended-Spectrum-5 (GES-5) producers, the first to be described in Enterobacteriaceae in the UK. The four NDM-producing C. freundii, two NDM-producing E. cloacae complex and 4/5 GES-5-producing E. cloacae complex were each indistinguishable isolates of the same three strains,whereas the six GES-5-producing K. oxytoca overall shared 79% similarity.Conclusion: CPE are readily isolated from hospital wastewater using simple culture methods. There are either undetected carriers of CPE excreting into the wastewater, or these CPE represent colonisation of the pipework. Surveillance of hospital wastewater for CPE does not appear helpful for infection control purposes.",
keywords = "Guiana-Extended-Spectrum-5 (GES-5), Metallo-β-lactamase, Carbapenem resistance, Surveillance",
author = "Leila White and Katie Hopkins and Dani{\`e}le Meunier and Perry, {Claire L.} and Rachel Pike and Paul Wilkinson and Pickup, {Roger William} and John Cheesbrough and Neil Woodford",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Journal of Hospital Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hospital Infection, 93, 2, 2016 DOI: 10.1016/j.jhin.2016.03.007",
year = "2016",
month = jun,
doi = "10.1016/j.jhin.2016.03.007",
language = "English",
volume = "93",
pages = "145--151",
journal = "Journal of Hospital Infection",
issn = "0195-6701",
publisher = "W.B. Saunders Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Carbapenemase-producing Enterobacteriaceae in hospital wastewater

T2 - a reservoir that may be unrelated to clinical isolates [star]

AU - White, Leila

AU - Hopkins, Katie

AU - Meunier, Danièle

AU - Perry, Claire L.

AU - Pike, Rachel

AU - Wilkinson, Paul

AU - Pickup, Roger William

AU - Cheesbrough, John

AU - Woodford, Neil

N1 - This is the author’s version of a work that was accepted for publication in Journal of Hospital Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Hospital Infection, 93, 2, 2016 DOI: 10.1016/j.jhin.2016.03.007

PY - 2016/6

Y1 - 2016/6

N2 - SummaryBackground: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging infection control problem in hospitals worldwide. Identifying carriers can help reduce potential spread and infections.Aim: To assess whether testing hospital wastewater for CPE can supplement patient based screening for infection prevention purposes in a hospital without a recognised endemic CPE problem.Methods: Wastewater collected from hospital pipework on 16 occasions during February-March 2014 was screened for CPE using chromID®CARBA agar and chromID®CPS agar with a 10 μg ertapenem disc and combination disc testing. MICs were determined using British Society for Antimicrobial Chemotherapy methodology and carbapenemase genes detected by PCR or wholegenomesequencing. Selected isolates were typed by PFGE.Findings: Suspected CPE were recovered from all 16 wastewater samples. Of 17 isolates sent to Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, six (four Citrobacter freundii and two Enterobacter cloacae complex) were New Delhi metallo–beta-lactamase (NDM) producers and the remaining 11 (six Klebsiella oxytoca and five Enterobacter cloacae complex),Guiana-Extended-Spectrum-5 (GES-5) producers, the first to be described in Enterobacteriaceae in the UK. The four NDM-producing C. freundii, two NDM-producing E. cloacae complex and 4/5 GES-5-producing E. cloacae complex were each indistinguishable isolates of the same three strains,whereas the six GES-5-producing K. oxytoca overall shared 79% similarity.Conclusion: CPE are readily isolated from hospital wastewater using simple culture methods. There are either undetected carriers of CPE excreting into the wastewater, or these CPE represent colonisation of the pipework. Surveillance of hospital wastewater for CPE does not appear helpful for infection control purposes.

AB - SummaryBackground: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging infection control problem in hospitals worldwide. Identifying carriers can help reduce potential spread and infections.Aim: To assess whether testing hospital wastewater for CPE can supplement patient based screening for infection prevention purposes in a hospital without a recognised endemic CPE problem.Methods: Wastewater collected from hospital pipework on 16 occasions during February-March 2014 was screened for CPE using chromID®CARBA agar and chromID®CPS agar with a 10 μg ertapenem disc and combination disc testing. MICs were determined using British Society for Antimicrobial Chemotherapy methodology and carbapenemase genes detected by PCR or wholegenomesequencing. Selected isolates were typed by PFGE.Findings: Suspected CPE were recovered from all 16 wastewater samples. Of 17 isolates sent to Antimicrobial Resistance and Healthcare Associated Infections Reference Unit, six (four Citrobacter freundii and two Enterobacter cloacae complex) were New Delhi metallo–beta-lactamase (NDM) producers and the remaining 11 (six Klebsiella oxytoca and five Enterobacter cloacae complex),Guiana-Extended-Spectrum-5 (GES-5) producers, the first to be described in Enterobacteriaceae in the UK. The four NDM-producing C. freundii, two NDM-producing E. cloacae complex and 4/5 GES-5-producing E. cloacae complex were each indistinguishable isolates of the same three strains,whereas the six GES-5-producing K. oxytoca overall shared 79% similarity.Conclusion: CPE are readily isolated from hospital wastewater using simple culture methods. There are either undetected carriers of CPE excreting into the wastewater, or these CPE represent colonisation of the pipework. Surveillance of hospital wastewater for CPE does not appear helpful for infection control purposes.

KW - Guiana-Extended-Spectrum-5 (GES-5)

KW - Metallo-β-lactamase

KW - Carbapenem resistance

KW - Surveillance

U2 - 10.1016/j.jhin.2016.03.007

DO - 10.1016/j.jhin.2016.03.007

M3 - Journal article

VL - 93

SP - 145

EP - 151

JO - Journal of Hospital Infection

JF - Journal of Hospital Infection

SN - 0195-6701

IS - 2

ER -