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Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line.

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Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line. / OWEN, P J ; MUSK, P ; EVANS, C A et al.
In: Journal of Biological Chemistry, Vol. 268, No. 21, 25.07.1993, p. 15696-15703.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

OWEN, PJ, MUSK, P, EVANS, CA & WHETTON, AD 1993, 'Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line.', Journal of Biological Chemistry, vol. 268, no. 21, pp. 15696-15703. <http://www.jbc.org/content/268/21/15696.abstract>

APA

OWEN, P. J., MUSK, P., EVANS, C. A., & WHETTON, A. D. (1993). Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line. Journal of Biological Chemistry, 268(21), 15696-15703. http://www.jbc.org/content/268/21/15696.abstract

Vancouver

OWEN PJ, MUSK P, EVANS CA, WHETTON AD. Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line. Journal of Biological Chemistry. 1993 Jul 25;268(21):15696-15703.

Author

OWEN, P J ; MUSK, P ; EVANS, C A et al. / Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 21. pp. 15696-15703.

Bibtex

@article{5ab8da00490b49fba9cda041cbfcd432,
title = "Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line.",
abstract = "A temperature-sensitive mutant of the v-abl oncoprotein has previously been shown to have markedly reduced tyrosine protein kinase activity in interleukin 3 (IL-3)-dependent cells grown at restrictive (39-degrees-C), compared to permissive (32-degrees-C) temperatures. Transfection of this mutant v-abl into the IC2.9 cell line, generated the IC.DP subclone which was dependent on IL-3 for survival at 39-degrees-C, but not at 32-degrees-C. Furthermore, IC.DP cells cultured at 32-degrees-C exhibited IL-3-independent thymidine incorporation, which was not apparent at 39-degrees-C. Switching cells from the restrictive to the permissive temperature resulted in an increase in cellular inositol-1,4,5-trisphosphate, choline phosphate and diacylglycerol levels in the IC.DP cell line. These increases were only observed after a lag period of 4 h. Within 2 h of switching IC.DP cells previously maintained at 32 to 39-degrees-C, there was a significant decrease in all three metabolites. Temperature switches had no effect upon these metabolites in the parent IC2.9 cell line. Down-regulation of protein kinase C inhibited v-abl-stimulated DNA synthesis in IC.DP cells cultured at 32-degrees-C. IC.DP cells cultured at 32-degrees-C were found to have a constitutively activated Na+/H+ antiport, although this activation was inhibited by the down-modulation of protein kinase C. These data indicate a role for phospholipid hydrolysis and protein kinase C activation in V-ABL-mediated abrogation of IL-3 dependence.",
author = "OWEN, {P J} and P MUSK and EVANS, {C A} and WHETTON, {A D}",
year = "1993",
month = jul,
day = "25",
language = "English",
volume = "268",
pages = "15696--15703",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "21",

}

RIS

TY - JOUR

T1 - Cellular signaling events elicited by v-abl associated with growth factor independence in an interleukin-3-dependent cell line.

AU - OWEN, P J

AU - MUSK, P

AU - EVANS, C A

AU - WHETTON, A D

PY - 1993/7/25

Y1 - 1993/7/25

N2 - A temperature-sensitive mutant of the v-abl oncoprotein has previously been shown to have markedly reduced tyrosine protein kinase activity in interleukin 3 (IL-3)-dependent cells grown at restrictive (39-degrees-C), compared to permissive (32-degrees-C) temperatures. Transfection of this mutant v-abl into the IC2.9 cell line, generated the IC.DP subclone which was dependent on IL-3 for survival at 39-degrees-C, but not at 32-degrees-C. Furthermore, IC.DP cells cultured at 32-degrees-C exhibited IL-3-independent thymidine incorporation, which was not apparent at 39-degrees-C. Switching cells from the restrictive to the permissive temperature resulted in an increase in cellular inositol-1,4,5-trisphosphate, choline phosphate and diacylglycerol levels in the IC.DP cell line. These increases were only observed after a lag period of 4 h. Within 2 h of switching IC.DP cells previously maintained at 32 to 39-degrees-C, there was a significant decrease in all three metabolites. Temperature switches had no effect upon these metabolites in the parent IC2.9 cell line. Down-regulation of protein kinase C inhibited v-abl-stimulated DNA synthesis in IC.DP cells cultured at 32-degrees-C. IC.DP cells cultured at 32-degrees-C were found to have a constitutively activated Na+/H+ antiport, although this activation was inhibited by the down-modulation of protein kinase C. These data indicate a role for phospholipid hydrolysis and protein kinase C activation in V-ABL-mediated abrogation of IL-3 dependence.

AB - A temperature-sensitive mutant of the v-abl oncoprotein has previously been shown to have markedly reduced tyrosine protein kinase activity in interleukin 3 (IL-3)-dependent cells grown at restrictive (39-degrees-C), compared to permissive (32-degrees-C) temperatures. Transfection of this mutant v-abl into the IC2.9 cell line, generated the IC.DP subclone which was dependent on IL-3 for survival at 39-degrees-C, but not at 32-degrees-C. Furthermore, IC.DP cells cultured at 32-degrees-C exhibited IL-3-independent thymidine incorporation, which was not apparent at 39-degrees-C. Switching cells from the restrictive to the permissive temperature resulted in an increase in cellular inositol-1,4,5-trisphosphate, choline phosphate and diacylglycerol levels in the IC.DP cell line. These increases were only observed after a lag period of 4 h. Within 2 h of switching IC.DP cells previously maintained at 32 to 39-degrees-C, there was a significant decrease in all three metabolites. Temperature switches had no effect upon these metabolites in the parent IC2.9 cell line. Down-regulation of protein kinase C inhibited v-abl-stimulated DNA synthesis in IC.DP cells cultured at 32-degrees-C. IC.DP cells cultured at 32-degrees-C were found to have a constitutively activated Na+/H+ antiport, although this activation was inhibited by the down-modulation of protein kinase C. These data indicate a role for phospholipid hydrolysis and protein kinase C activation in V-ABL-mediated abrogation of IL-3 dependence.

M3 - Journal article

VL - 268

SP - 15696

EP - 15703

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 21

ER -