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Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Cerebral ischemia-reperfusion induces the expression of phoenixin receptor (GPR173) and adult neurogenesis marker proteins in the rat striatum
AU - Mordecka-Chamera, Kinga
AU - Pałasz, Artur
AU - Suszka-Świtek, Aleksandra
AU - Bogus, Katarzyna
AU - Skałba, Władysław
AU - Piwowarczyk-Nowak, Aneta
AU - Worthington, John J.
AU - Pukowiec, Marta
AU - Sharma, Veerta
AU - Filipczyk, Łukasz
PY - 2024/12/29
Y1 - 2024/12/29
N2 - ObjectiveBrain ischemia is considered an extremely potent stress factor at the cellular and molecular level which may lead to massive neuronal death. Alternatively, short brain ischemia and reperfusion (I/R) can actually stimulate neurogenesis, angiogenesis and peptidergic signaling. There is little known about the potential effect of I/R on brain expression of the novel neuropeptide; phoenixin (PNX) and its receptor GPR173.MethodsThe study was carried out on adult male Wistar rats divided into seven groups: control, sham operation and 5 ischemic experimental groups across the time course of reperfusion. We examined mRNA and protein expression of GPR173 and neurogenesis markers Musashi-1, doublecortin (DCX), and Sox-2 in the striatum.ResultsGPR-173 positive cells were found only in the ischemic hemisphere, where Musashi-1, DCX and Sox-2-positive cells were also observed. Gene expression analysis also showed a significant increase of GPR-173 mRNA level in the I/R striatum in comparison with the control one. Results confirm previous findings suggesting that I/R stimulates adult neurogenesis in the striatum and affects peptidergic signaling in this structure.ConclusionsA very fast occurence of GPR-173 expression revealed in the striatum may potentially be exclusively related to neuroprotective neurochemical changes that occur in this region after I/R.
AB - ObjectiveBrain ischemia is considered an extremely potent stress factor at the cellular and molecular level which may lead to massive neuronal death. Alternatively, short brain ischemia and reperfusion (I/R) can actually stimulate neurogenesis, angiogenesis and peptidergic signaling. There is little known about the potential effect of I/R on brain expression of the novel neuropeptide; phoenixin (PNX) and its receptor GPR173.MethodsThe study was carried out on adult male Wistar rats divided into seven groups: control, sham operation and 5 ischemic experimental groups across the time course of reperfusion. We examined mRNA and protein expression of GPR173 and neurogenesis markers Musashi-1, doublecortin (DCX), and Sox-2 in the striatum.ResultsGPR-173 positive cells were found only in the ischemic hemisphere, where Musashi-1, DCX and Sox-2-positive cells were also observed. Gene expression analysis also showed a significant increase of GPR-173 mRNA level in the I/R striatum in comparison with the control one. Results confirm previous findings suggesting that I/R stimulates adult neurogenesis in the striatum and affects peptidergic signaling in this structure.ConclusionsA very fast occurence of GPR-173 expression revealed in the striatum may potentially be exclusively related to neuroprotective neurochemical changes that occur in this region after I/R.
KW - GPR173
KW - Phoenixin
KW - adult neurogenesis
KW - ischemia-reperfusion
KW - striatum
U2 - 10.1080/02699052.2024.2443004
DO - 10.1080/02699052.2024.2443004
M3 - Journal article
VL - 39
SP - 457
EP - 463
JO - Brain Injury
JF - Brain Injury
SN - 0269-9052
IS - 6
ER -