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Characterisation of oligosaccharides from the chondroitin/dermatan sulphates: 1H and 13C NMR studies of oligosaccharides generated by nitrous acid depolymerisation

Research output: Contribution to Journal/MagazineJournal articlepeer-review

<mark>Journal publication date</mark>18/10/2011
<mark>Journal</mark>Carbohydrate Research
Issue number14
Number of pages6
Pages (from-to)2222-2227
Publication StatusPublished
Early online date2/07/11
<mark>Original language</mark>English


The polymers chondroitin sulphate and dermatan sulphate have been fragmented by an anhydrous hydrazine/nitrous acid procedure. The resulting disaccharides from the polymer repeat sequences were reduced with NaBH4 and purified by ion exchange chromatography. Whereas enzymatic depolymerisation leads to the loss of the distinction between glucuronic and iduronic acids of CS and DS in the resultant disaccharides, nitrous acid depolymerisation retains these structures. Complete 1H and 13C NMR data have been derived for the major components which were shown to have the structures: GlcA(b1?3)- anTal6S-ol (I) and L-IdoA(a1?3)anTal4S-ol (II), where anTal-ol represents (2,5)anhydro-D-talitol and 6S/4S represent O-ester sulphate groups at C-6/C-4 sites.