Final published version
Research output: Contribution to Journal/Magazine › Review article › peer-review
Research output: Contribution to Journal/Magazine › Review article › peer-review
}
TY - JOUR
T1 - Characterisation of structural changes in collagen with Raman spectroscopy
AU - Martinez, Marcela Garcia
AU - Bullock, Anthony J.
AU - MacNeil, Sheila
AU - Rehman, Ihtesham U.
PY - 2019/7/3
Y1 - 2019/7/3
N2 - Raman spectroscopy can detect conformational changes in collagen structures and these findings are reviewed in this article. More specifically, some progressive diseases are mainly caused by alterations of collagen molecules but what is occurring at the biochemical level of this complex molecule usually remains unclear. While it may be true that a number of analytical techniques can analyze collagen, most of them have a series of limitations that limit their applicability to a wide range of samples. To understand in more detail the progression of a disease due to changes in the collagen structure, a technique that can detect subtle alterations at the biochemical level is needed. Raman spectroscopy is a label-free and noninvasive technique that can easily pick up on any conformational changes reflected primarily at the lipids, amides and proline and hydroxyproline regions. This review is the first compilation of studies of conformational changes in collagen molecules, providing help to understand changes in collagen biochemistry that can be of relevance to the human wound healing, ageing and pathologies.
AB - Raman spectroscopy can detect conformational changes in collagen structures and these findings are reviewed in this article. More specifically, some progressive diseases are mainly caused by alterations of collagen molecules but what is occurring at the biochemical level of this complex molecule usually remains unclear. While it may be true that a number of analytical techniques can analyze collagen, most of them have a series of limitations that limit their applicability to a wide range of samples. To understand in more detail the progression of a disease due to changes in the collagen structure, a technique that can detect subtle alterations at the biochemical level is needed. Raman spectroscopy is a label-free and noninvasive technique that can easily pick up on any conformational changes reflected primarily at the lipids, amides and proline and hydroxyproline regions. This review is the first compilation of studies of conformational changes in collagen molecules, providing help to understand changes in collagen biochemistry that can be of relevance to the human wound healing, ageing and pathologies.
KW - aldehydes
KW - collagen
KW - collagen crosslinking
KW - lysyl residues
KW - Raman spectroscopy
KW - secondary structure
U2 - 10.1080/05704928.2018.1506799
DO - 10.1080/05704928.2018.1506799
M3 - Review article
AN - SCOPUS:85068446571
VL - 54
SP - 509
EP - 542
JO - APPLIED SPECTROSCOPY REVIEWS
JF - APPLIED SPECTROSCOPY REVIEWS
SN - 0570-4928
IS - 6
ER -