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Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes

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Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes. / Hatano, Tomoyuki ; Palani, Saravanan ; Papatziamou, Dimitra et al.
In: Biorxiv, 17.08.2021.

Research output: Contribution to Journal/MagazineJournal article

Harvard

Hatano, T, Palani, S, Papatziamou, D, Souza, D, Salzer, R, Tamarit, D, Makwana, M, Potter, A, Haig, A, Xu, W, Townsend, D, Rochester, D, Bellini, D, Hussain, H, Ettema, T, Löwe, J, Baum, B, Robinson, N & Balasubramanian, M 2021, 'Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes', Biorxiv. https://doi.org/10.1101/2021.08.17.456605

APA

Hatano, T., Palani, S., Papatziamou, D., Souza, D., Salzer, R., Tamarit, D., Makwana, M., Potter, A., Haig, A., Xu, W., Townsend, D., Rochester, D., Bellini, D., Hussain, H., Ettema, T., Löwe, J., Baum, B., Robinson, N., & Balasubramanian, M. (2021). Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes. Biorxiv. https://doi.org/10.1101/2021.08.17.456605

Vancouver

Hatano T, Palani S, Papatziamou D, Souza D, Salzer R, Tamarit D et al. Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes. Biorxiv. 2021 Aug 17. doi: 10.1101/2021.08.17.456605

Author

Hatano, Tomoyuki ; Palani, Saravanan ; Papatziamou, Dimitra et al. / Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes. In: Biorxiv. 2021.

Bibtex

@article{955ab6c40e5e456a87a6579a4f464f7c,
title = "Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes",
abstract = "The ESCRT machinery performs a critical role in membrane remodelling events in all eukaryotic cells, including in membrane trafficking, membrane repair, cytokinetic abscission, in viral egress, and in the generation of extracellular vesicles. While the machinery is complex in modern day eukaryotes, where it comprises dozens of proteins, the system has simpler and more ancient origins. Indeed, homologues of ESCRT-III and the Vps4 ATPase, the proteins that execute the final membrane scission reaction, play analogous roles in cytokinesis and potentially in extracellular vesicle formation in TACK archaea where ESCRT-I and II homologues seem to be absent. Here, we explore the phylogeny, structure, and biochemistry of homologues of the ESCRT machinery and the associated ubiquitylation system found in genome assemblies of the recently discovered Asgard archaea. In these closest living prokaryotic relatives of eukaryotes, we provide evidence for the ESCRT-I and II sub-complexes being involved in the ubiquitin-directed recruitment of ESCRT-III,_as it is in eukaryotes. This analysis suggests a pre-eukaryotic origin for the Ub-coupled ESCRT system and a likely path of ESCRT evolution via a series of gene duplication and diversification events.",
author = "Tomoyuki Hatano and Saravanan Palani and Dimitra Papatziamou and Diorge Souza and Ralf Salzer and Daniel Tamarit and Mehul Makwana and Antonia Potter and Alexandra Haig and Wenjue Xu and David Townsend and David Rochester and Dom Bellini and Hamdi Hussain and Thijs Ettema and Jan L{\"o}we and Buzz Baum and Nick Robinson and Mohan Balasubramanian",
year = "2021",
month = aug,
day = "17",
doi = "10.1101/2021.08.17.456605",
language = "English",
journal = "Biorxiv",
publisher = "Cold Spring Harbor Laboratory Press",

}

RIS

TY - JOUR

T1 - Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes

AU - Hatano, Tomoyuki

AU - Palani, Saravanan

AU - Papatziamou, Dimitra

AU - Souza, Diorge

AU - Salzer, Ralf

AU - Tamarit, Daniel

AU - Makwana, Mehul

AU - Potter, Antonia

AU - Haig, Alexandra

AU - Xu, Wenjue

AU - Townsend, David

AU - Rochester, David

AU - Bellini, Dom

AU - Hussain, Hamdi

AU - Ettema, Thijs

AU - Löwe, Jan

AU - Baum, Buzz

AU - Robinson, Nick

AU - Balasubramanian, Mohan

PY - 2021/8/17

Y1 - 2021/8/17

N2 - The ESCRT machinery performs a critical role in membrane remodelling events in all eukaryotic cells, including in membrane trafficking, membrane repair, cytokinetic abscission, in viral egress, and in the generation of extracellular vesicles. While the machinery is complex in modern day eukaryotes, where it comprises dozens of proteins, the system has simpler and more ancient origins. Indeed, homologues of ESCRT-III and the Vps4 ATPase, the proteins that execute the final membrane scission reaction, play analogous roles in cytokinesis and potentially in extracellular vesicle formation in TACK archaea where ESCRT-I and II homologues seem to be absent. Here, we explore the phylogeny, structure, and biochemistry of homologues of the ESCRT machinery and the associated ubiquitylation system found in genome assemblies of the recently discovered Asgard archaea. In these closest living prokaryotic relatives of eukaryotes, we provide evidence for the ESCRT-I and II sub-complexes being involved in the ubiquitin-directed recruitment of ESCRT-III,_as it is in eukaryotes. This analysis suggests a pre-eukaryotic origin for the Ub-coupled ESCRT system and a likely path of ESCRT evolution via a series of gene duplication and diversification events.

AB - The ESCRT machinery performs a critical role in membrane remodelling events in all eukaryotic cells, including in membrane trafficking, membrane repair, cytokinetic abscission, in viral egress, and in the generation of extracellular vesicles. While the machinery is complex in modern day eukaryotes, where it comprises dozens of proteins, the system has simpler and more ancient origins. Indeed, homologues of ESCRT-III and the Vps4 ATPase, the proteins that execute the final membrane scission reaction, play analogous roles in cytokinesis and potentially in extracellular vesicle formation in TACK archaea where ESCRT-I and II homologues seem to be absent. Here, we explore the phylogeny, structure, and biochemistry of homologues of the ESCRT machinery and the associated ubiquitylation system found in genome assemblies of the recently discovered Asgard archaea. In these closest living prokaryotic relatives of eukaryotes, we provide evidence for the ESCRT-I and II sub-complexes being involved in the ubiquitin-directed recruitment of ESCRT-III,_as it is in eukaryotes. This analysis suggests a pre-eukaryotic origin for the Ub-coupled ESCRT system and a likely path of ESCRT evolution via a series of gene duplication and diversification events.

U2 - 10.1101/2021.08.17.456605

DO - 10.1101/2021.08.17.456605

M3 - Journal article

JO - Biorxiv

JF - Biorxiv

ER -