Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1
AU - Zhang, Dayi
AU - Zhao, Yun
AU - He, Yi
AU - Wang, Yun
AU - Zhao, Yiyu
AU - Zheng, Yi
AU - Wei, Xia
AU - Zhang, Litong
AU - Li, Yuzhen
AU - Jin, Tao
AU - Wu, Lin
AU - Wang, Hui
AU - Davison, Paul A.
AU - Xu, Junguang
AU - Huang, Wei E.
PY - 2012/7
Y1 - 2012/7
N2 - Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP 1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.
AB - Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP 1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.
KW - GENES
KW - ESCHERICHIA-COLI
KW - OPERON
KW - benzoate
KW - catechol
KW - salicylate
KW - Acinetobacter baylyi ADP1
KW - ACTIVATOR
KW - LysR-type gene regulation
KW - TRANSFORMATION
KW - STRAIN ADP1
KW - repressor
KW - PROMOTER
KW - DEGRADATION
KW - BACTERIUM
KW - cross-regulation
KW - mathematic model
KW - SALICYLATE
U2 - 10.1021/sb3000244
DO - 10.1021/sb3000244
M3 - Journal article
VL - 1
SP - 274
EP - 283
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
SN - 2161-5063
IS - 7
ER -