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Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway. / Roberts, Michael; Hodge, Rachel; Ross, Jo H.E. et al.
In: Plant Journal, Vol. 3, No. 5, 05.1993, p. 629-636.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Roberts, M, Hodge, R, Ross, JHE, Sorensen, A, Murphy, DJ, Draper, J & Scott, R 1993, 'Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway', Plant Journal, vol. 3, no. 5, pp. 629-636. https://doi.org/10.1111/j.1365-313X.1993.00629.x

APA

Roberts, M., Hodge, R., Ross, J. H. E., Sorensen, A., Murphy, D. J., Draper, J., & Scott, R. (1993). Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway. Plant Journal, 3(5), 629-636. https://doi.org/10.1111/j.1365-313X.1993.00629.x

Vancouver

Roberts M, Hodge R, Ross JHE, Sorensen A, Murphy DJ, Draper J et al. Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway. Plant Journal. 1993 May;3(5):629-636. doi: 10.1111/j.1365-313X.1993.00629.x

Author

Roberts, Michael ; Hodge, Rachel ; Ross, Jo H.E. et al. / Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway. In: Plant Journal. 1993 ; Vol. 3, No. 5. pp. 629-636.

Bibtex

@article{455bb2a9828248f290577e755cd06f67,
title = "Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway",
abstract = "The expression and sequences of two related anther-specific cDNAs (I3 and C98) of Brassica napus (oilseed rape) were examined. These cDNAs were found to exhibit significant predicted amino acid sequence similarity with a group of seed-specific proteins, the oleosins, which are involved in oil body membranestructure. Pollen of B. napus also contains oil bodies, and the synthesis and accumulation of these organelles correlates with expression of the I3 and C98 transcripts. The protein content of purified pollen oil bodies was or absence of possible oleosins. One major protein species of 14 kDa was identified and subjected to N-terminal amino acid sequence determination. The sequence of this pollen oil body associated protein is homologous to the predicted sequence of one of the anther-specific cDNAs, I3, implying that this cDNA represents an anther-specific oleosin gene expressed in developing pollen. The expression of other genes active during seed oil body formation was also examined, and these were found to be specific to the sporophyte, inferring the existence of specificcomponents of the pathway of lipid synthesis and storage in the male gametophyte. These possibilities and the relationship between different oleosin genes are discussed.",
keywords = "pollen, oil body, oleosin, gametophyte",
author = "Michael Roberts and Rachel Hodge and Ross, {Jo H.E.} and Anna Sorensen and Murphy, {Denis J.} and John Draper and Rod Scott",
year = "1993",
month = may,
doi = "10.1111/j.1365-313X.1993.00629.x",
language = "English",
volume = "3",
pages = "629--636",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Blackwell Publishing Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - Characterization of a new class of oleosins suggests a male gametophyte-specific lipid storage pathway

AU - Roberts, Michael

AU - Hodge, Rachel

AU - Ross, Jo H.E.

AU - Sorensen, Anna

AU - Murphy, Denis J.

AU - Draper, John

AU - Scott, Rod

PY - 1993/5

Y1 - 1993/5

N2 - The expression and sequences of two related anther-specific cDNAs (I3 and C98) of Brassica napus (oilseed rape) were examined. These cDNAs were found to exhibit significant predicted amino acid sequence similarity with a group of seed-specific proteins, the oleosins, which are involved in oil body membranestructure. Pollen of B. napus also contains oil bodies, and the synthesis and accumulation of these organelles correlates with expression of the I3 and C98 transcripts. The protein content of purified pollen oil bodies was or absence of possible oleosins. One major protein species of 14 kDa was identified and subjected to N-terminal amino acid sequence determination. The sequence of this pollen oil body associated protein is homologous to the predicted sequence of one of the anther-specific cDNAs, I3, implying that this cDNA represents an anther-specific oleosin gene expressed in developing pollen. The expression of other genes active during seed oil body formation was also examined, and these were found to be specific to the sporophyte, inferring the existence of specificcomponents of the pathway of lipid synthesis and storage in the male gametophyte. These possibilities and the relationship between different oleosin genes are discussed.

AB - The expression and sequences of two related anther-specific cDNAs (I3 and C98) of Brassica napus (oilseed rape) were examined. These cDNAs were found to exhibit significant predicted amino acid sequence similarity with a group of seed-specific proteins, the oleosins, which are involved in oil body membranestructure. Pollen of B. napus also contains oil bodies, and the synthesis and accumulation of these organelles correlates with expression of the I3 and C98 transcripts. The protein content of purified pollen oil bodies was or absence of possible oleosins. One major protein species of 14 kDa was identified and subjected to N-terminal amino acid sequence determination. The sequence of this pollen oil body associated protein is homologous to the predicted sequence of one of the anther-specific cDNAs, I3, implying that this cDNA represents an anther-specific oleosin gene expressed in developing pollen. The expression of other genes active during seed oil body formation was also examined, and these were found to be specific to the sporophyte, inferring the existence of specificcomponents of the pathway of lipid synthesis and storage in the male gametophyte. These possibilities and the relationship between different oleosin genes are discussed.

KW - pollen

KW - oil body

KW - oleosin

KW - gametophyte

U2 - 10.1111/j.1365-313X.1993.00629.x

DO - 10.1111/j.1365-313X.1993.00629.x

M3 - Journal article

VL - 3

SP - 629

EP - 636

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 5

ER -