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Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9.

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Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9. / Caspari, Thomas; Dahlen, Maria; Kanter-Smoler, Gunilla; Lindsay, Howard D.; Hofmann, Kay; Papadimitriou, Konstantinos; Sunnerhagen, Per; Carr, Antony M.

In: Molecular and Cellular Biology, Vol. 20, No. 4, 02.2000, p. 1254-1262.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Caspari, T, Dahlen, M, Kanter-Smoler, G, Lindsay, HD, Hofmann, K, Papadimitriou, K, Sunnerhagen, P & Carr, AM 2000, 'Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9.', Molecular and Cellular Biology, vol. 20, no. 4, pp. 1254-1262. <http://mcb.asm.org/cgi/content/abstract/20/4/1254>

APA

Caspari, T., Dahlen, M., Kanter-Smoler, G., Lindsay, H. D., Hofmann, K., Papadimitriou, K., Sunnerhagen, P., & Carr, A. M. (2000). Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9. Molecular and Cellular Biology, 20(4), 1254-1262. http://mcb.asm.org/cgi/content/abstract/20/4/1254

Vancouver

Caspari T, Dahlen M, Kanter-Smoler G, Lindsay HD, Hofmann K, Papadimitriou K et al. Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9. Molecular and Cellular Biology. 2000 Feb;20(4):1254-1262.

Author

Caspari, Thomas ; Dahlen, Maria ; Kanter-Smoler, Gunilla ; Lindsay, Howard D. ; Hofmann, Kay ; Papadimitriou, Konstantinos ; Sunnerhagen, Per ; Carr, Antony M. / Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 4. pp. 1254-1262.

Bibtex

@article{7d16897024f34091a1378f710f50cb20,
title = "Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9.",
abstract = "Hus1 is one of six checkpoint Rad proteins required for all Schizosaccharomyces pombe DNA integrity checkpoints. MYC-tagged Hus1 reveals four discrete forms. The main form, Hus1-B, participates in a protein complex with Rad9 and Rad1, consistent with reports that Rad1-Hus1 immunoprecipitation is dependent on the rad9+ locus. A small proportion of Hus1-B is intrinsically phosphorylated in undamaged cells and more becomes phosphorylated after irradiation. Hus1-B phosphorylation is not increased in cells blocked in early S phase with hydroxyurea unless exposure is prolonged. The Rad1-Rad9-Hus1-B complex is readily detectable, but upon cofractionation of soluble extracts, the majority of each protein is not present in this complex. Indirect immunofluorescence demonstrates that Hus1 is nuclear and that this localization depends on Rad17. We show that Rad17 defines a distinct protein complex in soluble extracts that is separate from Rad1, Rad9, and Hus1. However, two-hybrid interaction, in vitro association and in vivo overexpression experiments suggest a transient interaction between Rad1 and Rad17.",
author = "Thomas Caspari and Maria Dahlen and Gunilla Kanter-Smoler and Lindsay, {Howard D.} and Kay Hofmann and Konstantinos Papadimitriou and Per Sunnerhagen and Carr, {Antony M.}",
year = "2000",
month = feb,
language = "English",
volume = "20",
pages = "1254--1262",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "4",

}

RIS

TY - JOUR

T1 - Characterization of Schizosaccharomyces pombe Hus1: a PCNA-Related Protein That Associates with Rad1 and Rad9.

AU - Caspari, Thomas

AU - Dahlen, Maria

AU - Kanter-Smoler, Gunilla

AU - Lindsay, Howard D.

AU - Hofmann, Kay

AU - Papadimitriou, Konstantinos

AU - Sunnerhagen, Per

AU - Carr, Antony M.

PY - 2000/2

Y1 - 2000/2

N2 - Hus1 is one of six checkpoint Rad proteins required for all Schizosaccharomyces pombe DNA integrity checkpoints. MYC-tagged Hus1 reveals four discrete forms. The main form, Hus1-B, participates in a protein complex with Rad9 and Rad1, consistent with reports that Rad1-Hus1 immunoprecipitation is dependent on the rad9+ locus. A small proportion of Hus1-B is intrinsically phosphorylated in undamaged cells and more becomes phosphorylated after irradiation. Hus1-B phosphorylation is not increased in cells blocked in early S phase with hydroxyurea unless exposure is prolonged. The Rad1-Rad9-Hus1-B complex is readily detectable, but upon cofractionation of soluble extracts, the majority of each protein is not present in this complex. Indirect immunofluorescence demonstrates that Hus1 is nuclear and that this localization depends on Rad17. We show that Rad17 defines a distinct protein complex in soluble extracts that is separate from Rad1, Rad9, and Hus1. However, two-hybrid interaction, in vitro association and in vivo overexpression experiments suggest a transient interaction between Rad1 and Rad17.

AB - Hus1 is one of six checkpoint Rad proteins required for all Schizosaccharomyces pombe DNA integrity checkpoints. MYC-tagged Hus1 reveals four discrete forms. The main form, Hus1-B, participates in a protein complex with Rad9 and Rad1, consistent with reports that Rad1-Hus1 immunoprecipitation is dependent on the rad9+ locus. A small proportion of Hus1-B is intrinsically phosphorylated in undamaged cells and more becomes phosphorylated after irradiation. Hus1-B phosphorylation is not increased in cells blocked in early S phase with hydroxyurea unless exposure is prolonged. The Rad1-Rad9-Hus1-B complex is readily detectable, but upon cofractionation of soluble extracts, the majority of each protein is not present in this complex. Indirect immunofluorescence demonstrates that Hus1 is nuclear and that this localization depends on Rad17. We show that Rad17 defines a distinct protein complex in soluble extracts that is separate from Rad1, Rad9, and Hus1. However, two-hybrid interaction, in vitro association and in vivo overexpression experiments suggest a transient interaction between Rad1 and Rad17.

M3 - Journal article

VL - 20

SP - 1254

EP - 1262

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 4

ER -