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Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse.

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Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse. / Hodges, Matt; Vieira, Helena; Gregory-Evans, Kevin et al.
In: Genomics, Vol. 80, No. 5, 11.2002, p. 531-542.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Hodges, M, Vieira, H, Gregory-Evans, K & Gregory-Evans, CY 2002, 'Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse.', Genomics, vol. 80, no. 5, pp. 531-542. https://doi.org/10.1006/geno.2002.6854

APA

Hodges, M., Vieira, H., Gregory-Evans, K., & Gregory-Evans, C. Y. (2002). Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse. Genomics, 80(5), 531-542. https://doi.org/10.1006/geno.2002.6854

Vancouver

Hodges M, Vieira H, Gregory-Evans K, Gregory-Evans CY. Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse. Genomics. 2002 Nov;80(5):531-542. doi: 10.1006/geno.2002.6854

Author

Hodges, Matt ; Vieira, Helena ; Gregory-Evans, Kevin et al. / Characterization of the genomic and transcriptional structure of the CRX gene : substantial differences between human and mouse. In: Genomics. 2002 ; Vol. 80, No. 5. pp. 531-542.

Bibtex

@article{b0ea488bab0c4c2e876089e8c47f0792,
title = "Characterization of the genomic and transcriptional structure of the CRX gene: substantial differences between human and mouse.",
abstract = "We have previously shown that there is a temporal difference in human CRX: gene expression compared with that of mouse Crx. We have now characterized these genes at the genomic and transcriptional levels and here we expand on this earlier report. Human CRX: spans 25 kb and has six exons, and mouse Crx: spans 15 kb and has four exons. We isolated seven human and two mouse mRNAs generated by alternative splicing of a variable 5′ untranslated region. The human and mouse genes share an evolutionarily conserved promoter, which contains OTX/CRX type and SP1/AP2 binding sites and drives expression of two conserved transcripts in both species. Additionally, the human gene has a second human-specific promoter, which has OTX/CRX type binding sites and drives expression of five other transcripts. Band shift assays have shown that six of the seven candidate OTX/CRX elements bind CRX in vitro, possibly implying that the gene can regulate its own expression. These data may account for the differences in temporal expression in vivo: we have previously reported between these two species.",
keywords = "CRX, eye",
author = "Matt Hodges and Helena Vieira and Kevin Gregory-Evans and Gregory-Evans, {Cheryl Y.}",
year = "2002",
month = nov,
doi = "10.1006/geno.2002.6854",
language = "English",
volume = "80",
pages = "531--542",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "5",

}

RIS

TY - JOUR

T1 - Characterization of the genomic and transcriptional structure of the CRX gene

T2 - substantial differences between human and mouse.

AU - Hodges, Matt

AU - Vieira, Helena

AU - Gregory-Evans, Kevin

AU - Gregory-Evans, Cheryl Y.

PY - 2002/11

Y1 - 2002/11

N2 - We have previously shown that there is a temporal difference in human CRX: gene expression compared with that of mouse Crx. We have now characterized these genes at the genomic and transcriptional levels and here we expand on this earlier report. Human CRX: spans 25 kb and has six exons, and mouse Crx: spans 15 kb and has four exons. We isolated seven human and two mouse mRNAs generated by alternative splicing of a variable 5′ untranslated region. The human and mouse genes share an evolutionarily conserved promoter, which contains OTX/CRX type and SP1/AP2 binding sites and drives expression of two conserved transcripts in both species. Additionally, the human gene has a second human-specific promoter, which has OTX/CRX type binding sites and drives expression of five other transcripts. Band shift assays have shown that six of the seven candidate OTX/CRX elements bind CRX in vitro, possibly implying that the gene can regulate its own expression. These data may account for the differences in temporal expression in vivo: we have previously reported between these two species.

AB - We have previously shown that there is a temporal difference in human CRX: gene expression compared with that of mouse Crx. We have now characterized these genes at the genomic and transcriptional levels and here we expand on this earlier report. Human CRX: spans 25 kb and has six exons, and mouse Crx: spans 15 kb and has four exons. We isolated seven human and two mouse mRNAs generated by alternative splicing of a variable 5′ untranslated region. The human and mouse genes share an evolutionarily conserved promoter, which contains OTX/CRX type and SP1/AP2 binding sites and drives expression of two conserved transcripts in both species. Additionally, the human gene has a second human-specific promoter, which has OTX/CRX type binding sites and drives expression of five other transcripts. Band shift assays have shown that six of the seven candidate OTX/CRX elements bind CRX in vitro, possibly implying that the gene can regulate its own expression. These data may account for the differences in temporal expression in vivo: we have previously reported between these two species.

KW - CRX

KW - eye

U2 - 10.1006/geno.2002.6854

DO - 10.1006/geno.2002.6854

M3 - Journal article

VL - 80

SP - 531

EP - 542

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 5

ER -