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Characterization of the key pathways of dissimilatory nitrate reduction and their response to complex organic substrates in hyporheic sediments

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Characterization of the key pathways of dissimilatory nitrate reduction and their response to complex organic substrates in hyporheic sediments. / Lansdown, K; Trimmer, Mark; Heppell, C.M. et al.
In: Limnology and Oceanography, Vol. 57, No. 2, 03.2012, p. 387-400.

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Lansdown K, Trimmer M, Heppell CM, Sgouridis F, Ullah S, Heathwaite L et al. Characterization of the key pathways of dissimilatory nitrate reduction and their response to complex organic substrates in hyporheic sediments. Limnology and Oceanography. 2012 Mar;57(2):387-400. Epub 2012 Jan 30. doi: 10.4319/lo.2012.57.2.0387

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Lansdown, K ; Trimmer, Mark ; Heppell, C.M. et al. / Characterization of the key pathways of dissimilatory nitrate reduction and their response to complex organic substrates in hyporheic sediments. In: Limnology and Oceanography. 2012 ; Vol. 57, No. 2. pp. 387-400.

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@article{d4e17a3add5240459360f8816a5bd9ee,
title = "Characterization of the key pathways of dissimilatory nitrate reduction and their response to complex organic substrates in hyporheic sediments",
abstract = "ABSTRACT: Laboratory incubations with river-bed sediment collected from riffles and pools were used to quantify potential pathways of dissimilatory nitrate reduction in the hyporheic zone of a groundwater-fed river. Sediments collected from between 5-cm and 86-cm depth in the bed of the River Leith, Cumbria, United Kingdom, were incubated with a suite of 15N-labeled substrates (15NO-3, 15NH+4, and 14NO-3) to quantify nitrate reduction via denitrification, dissimilatory nitrate reduction to ammonium (DNRA), and anaerobic ammonium oxidation (anammox). Denitrification was the dominant pathway of dissimilatory nitrate reduction in the hyporheic sediments, although recovery of 15N from the ammonium pool indicated that DNRA was also active. The potential for anammox was confirmed by the production of 29N2 during the 15NH+4 and 14NO-3 incubation, but it was much smaller than denitrification. Potential rates of denitrification were highest in shallow sediments and decayed exponentially with depth thereafter. There were clear differences in denitrification activity between riffle and pool sediments. After the production of 15N-N2 had stabilized, we added a spike of bacteriological peptone to determine the effect of complex organic substrates on denitrification potential. The potential rate of denitrification increased uniformly at all sediment depths but the total amount of denitrification fueled by the organic substrates decreased markedly with depth, from 90% in the shallow sediments to 30% in the deepest sediments. In addition, a considerable fraction of the 15NO-3 could not be accounted for, which suggested that up to 87% of it had been assimilated in the deepest sediments. ",
author = "K Lansdown and Mark Trimmer and C.M. Heppell and F Sgouridis and Sami Ullah and Louise Heathwaite and Andrew Binley and Hao Zhang",
year = "2012",
month = mar,
doi = "10.4319/lo.2012.57.2.0387",
language = "English",
volume = "57",
pages = "387--400",
journal = "Limnology and Oceanography",
issn = "0024-3590",
publisher = "Wiley Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Characterization of the key pathways of dissimilatory nitrate reduction and their response to complex organic substrates in hyporheic sediments

AU - Lansdown, K

AU - Trimmer, Mark

AU - Heppell, C.M.

AU - Sgouridis, F

AU - Ullah, Sami

AU - Heathwaite, Louise

AU - Binley, Andrew

AU - Zhang, Hao

PY - 2012/3

Y1 - 2012/3

N2 - ABSTRACT: Laboratory incubations with river-bed sediment collected from riffles and pools were used to quantify potential pathways of dissimilatory nitrate reduction in the hyporheic zone of a groundwater-fed river. Sediments collected from between 5-cm and 86-cm depth in the bed of the River Leith, Cumbria, United Kingdom, were incubated with a suite of 15N-labeled substrates (15NO-3, 15NH+4, and 14NO-3) to quantify nitrate reduction via denitrification, dissimilatory nitrate reduction to ammonium (DNRA), and anaerobic ammonium oxidation (anammox). Denitrification was the dominant pathway of dissimilatory nitrate reduction in the hyporheic sediments, although recovery of 15N from the ammonium pool indicated that DNRA was also active. The potential for anammox was confirmed by the production of 29N2 during the 15NH+4 and 14NO-3 incubation, but it was much smaller than denitrification. Potential rates of denitrification were highest in shallow sediments and decayed exponentially with depth thereafter. There were clear differences in denitrification activity between riffle and pool sediments. After the production of 15N-N2 had stabilized, we added a spike of bacteriological peptone to determine the effect of complex organic substrates on denitrification potential. The potential rate of denitrification increased uniformly at all sediment depths but the total amount of denitrification fueled by the organic substrates decreased markedly with depth, from 90% in the shallow sediments to 30% in the deepest sediments. In addition, a considerable fraction of the 15NO-3 could not be accounted for, which suggested that up to 87% of it had been assimilated in the deepest sediments.

AB - ABSTRACT: Laboratory incubations with river-bed sediment collected from riffles and pools were used to quantify potential pathways of dissimilatory nitrate reduction in the hyporheic zone of a groundwater-fed river. Sediments collected from between 5-cm and 86-cm depth in the bed of the River Leith, Cumbria, United Kingdom, were incubated with a suite of 15N-labeled substrates (15NO-3, 15NH+4, and 14NO-3) to quantify nitrate reduction via denitrification, dissimilatory nitrate reduction to ammonium (DNRA), and anaerobic ammonium oxidation (anammox). Denitrification was the dominant pathway of dissimilatory nitrate reduction in the hyporheic sediments, although recovery of 15N from the ammonium pool indicated that DNRA was also active. The potential for anammox was confirmed by the production of 29N2 during the 15NH+4 and 14NO-3 incubation, but it was much smaller than denitrification. Potential rates of denitrification were highest in shallow sediments and decayed exponentially with depth thereafter. There were clear differences in denitrification activity between riffle and pool sediments. After the production of 15N-N2 had stabilized, we added a spike of bacteriological peptone to determine the effect of complex organic substrates on denitrification potential. The potential rate of denitrification increased uniformly at all sediment depths but the total amount of denitrification fueled by the organic substrates decreased markedly with depth, from 90% in the shallow sediments to 30% in the deepest sediments. In addition, a considerable fraction of the 15NO-3 could not be accounted for, which suggested that up to 87% of it had been assimilated in the deepest sediments.

U2 - 10.4319/lo.2012.57.2.0387

DO - 10.4319/lo.2012.57.2.0387

M3 - Journal article

VL - 57

SP - 387

EP - 400

JO - Limnology and Oceanography

JF - Limnology and Oceanography

SN - 0024-3590

IS - 2

ER -