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Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects

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Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects. / Jin, Minghui; Liu, Bo; Zheng, Weigang et al.
In: BMC Biology, Vol. 21, No. 1, 2, 05.01.2023.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Jin, M, Liu, B, Zheng, W, Liu, C, Liu, Z, He, Y, Li, X, Wu, C, Wang, P, Liu, K, Wu, S, Liu, H, Chakrabarty, S, Yuan, H, Wilson, K, Wu, K, Fan, W & Xiao, Y 2023, 'Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects', BMC Biology, vol. 21, no. 1, 2. https://doi.org/10.1186/s12915-022-01504-y

APA

Jin, M., Liu, B., Zheng, W., Liu, C., Liu, Z., He, Y., Li, X., Wu, C., Wang, P., Liu, K., Wu, S., Liu, H., Chakrabarty, S., Yuan, H., Wilson, K., Wu, K., Fan, W., & Xiao, Y. (2023). Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects. BMC Biology, 21(1), Article 2. https://doi.org/10.1186/s12915-022-01504-y

Vancouver

Jin M, Liu B, Zheng W, Liu C, Liu Z, He Y et al. Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects. BMC Biology. 2023 Jan 5;21(1):2. doi: 10.1186/s12915-022-01504-y

Author

Jin, Minghui ; Liu, Bo ; Zheng, Weigang et al. / Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects. In: BMC Biology. 2023 ; Vol. 21, No. 1.

Bibtex

@article{0379f91e249f4b7a8a4a3d9df715530d,
title = "Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects",
abstract = "Background: The black cutworm, Agrotis ipsilon, is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute to its widening distribution and increasing difficulty of control. However, knowledge about these traits is still limited. Results: We generated a high-quality chromosome-level assembly of A. ipsilon using PacBio and Hi-C technology with a contig N50 length of ~ 6.7 Mb. Comparative genomic and transcriptomic analyses showed that detoxification-associated gene families were highly expanded and induced after insects fed on specific host plants. Knockout of genes that encoded two induced ABC transporters using CRISPR/Cas9 significantly reduced larval growth rate, consistent with their contribution to host adaptation. A comparative transcriptomic analysis between tethered-flight moths and migrating moths showed expression changes in the circadian rhythm gene AiCry2 involved in sensing photoperiod variations and may receipt magnetic fields accompanied by MagR and in genes that regulate the juvenile hormone pathway and energy metabolism, all involved in migration processes. Conclusions: This study provides valuable genomic resources for elucidating the mechanisms involved in moth migration and developing innovative control strategies.",
keywords = "Research Article, Evolutionary Genomics, Genome assembly, Comparative genomics, Transcriptome, Host adaptation, Migration, Cutworm",
author = "Minghui Jin and Bo Liu and Weigang Zheng and Conghui Liu and Zhenxing Liu and Yuan He and Xiaokang Li and Chao Wu and Ping Wang and Kaiyu Liu and Shigang Wu and Hangwei Liu and Swapan Chakrabarty and Haibin Yuan and Kenneth Wilson and Kongming Wu and Wei Fan and Yutao Xiao",
year = "2023",
month = jan,
day = "5",
doi = "10.1186/s12915-022-01504-y",
language = "English",
volume = "21",
journal = "BMC Biology",
issn = "1741-7007",
publisher = "BioMed Central",
number = "1",

}

RIS

TY - JOUR

T1 - Chromosome-level genome of black cutworm provides novel insights into polyphagy and seasonal migration in insects

AU - Jin, Minghui

AU - Liu, Bo

AU - Zheng, Weigang

AU - Liu, Conghui

AU - Liu, Zhenxing

AU - He, Yuan

AU - Li, Xiaokang

AU - Wu, Chao

AU - Wang, Ping

AU - Liu, Kaiyu

AU - Wu, Shigang

AU - Liu, Hangwei

AU - Chakrabarty, Swapan

AU - Yuan, Haibin

AU - Wilson, Kenneth

AU - Wu, Kongming

AU - Fan, Wei

AU - Xiao, Yutao

PY - 2023/1/5

Y1 - 2023/1/5

N2 - Background: The black cutworm, Agrotis ipsilon, is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute to its widening distribution and increasing difficulty of control. However, knowledge about these traits is still limited. Results: We generated a high-quality chromosome-level assembly of A. ipsilon using PacBio and Hi-C technology with a contig N50 length of ~ 6.7 Mb. Comparative genomic and transcriptomic analyses showed that detoxification-associated gene families were highly expanded and induced after insects fed on specific host plants. Knockout of genes that encoded two induced ABC transporters using CRISPR/Cas9 significantly reduced larval growth rate, consistent with their contribution to host adaptation. A comparative transcriptomic analysis between tethered-flight moths and migrating moths showed expression changes in the circadian rhythm gene AiCry2 involved in sensing photoperiod variations and may receipt magnetic fields accompanied by MagR and in genes that regulate the juvenile hormone pathway and energy metabolism, all involved in migration processes. Conclusions: This study provides valuable genomic resources for elucidating the mechanisms involved in moth migration and developing innovative control strategies.

AB - Background: The black cutworm, Agrotis ipsilon, is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute to its widening distribution and increasing difficulty of control. However, knowledge about these traits is still limited. Results: We generated a high-quality chromosome-level assembly of A. ipsilon using PacBio and Hi-C technology with a contig N50 length of ~ 6.7 Mb. Comparative genomic and transcriptomic analyses showed that detoxification-associated gene families were highly expanded and induced after insects fed on specific host plants. Knockout of genes that encoded two induced ABC transporters using CRISPR/Cas9 significantly reduced larval growth rate, consistent with their contribution to host adaptation. A comparative transcriptomic analysis between tethered-flight moths and migrating moths showed expression changes in the circadian rhythm gene AiCry2 involved in sensing photoperiod variations and may receipt magnetic fields accompanied by MagR and in genes that regulate the juvenile hormone pathway and energy metabolism, all involved in migration processes. Conclusions: This study provides valuable genomic resources for elucidating the mechanisms involved in moth migration and developing innovative control strategies.

KW - Research Article

KW - Evolutionary Genomics

KW - Genome assembly

KW - Comparative genomics

KW - Transcriptome

KW - Host adaptation

KW - Migration

KW - Cutworm

U2 - 10.1186/s12915-022-01504-y

DO - 10.1186/s12915-022-01504-y

M3 - Journal article

VL - 21

JO - BMC Biology

JF - BMC Biology

SN - 1741-7007

IS - 1

M1 - 2

ER -