Final published version
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Chromosome-scale genome sequencing, assembly and annotation of six genomes from subfamily Leishmaniinae
AU - Almutairi, Hatim
AU - Urbaniak, Michael D
AU - Bates, Michelle D
AU - Jariyapan, Narissara
AU - Kwakye-Nuako, Godwin
AU - Thomaz Soccol, Vanete
AU - Al-Salem, Waleed S
AU - Dillon, Rod J
AU - Bates, Paul A
AU - Gatherer, Derek
N1 - © 2021. The Author(s).
PY - 2021/9/6
Y1 - 2021/9/6
N2 - We provide the raw and processed data produced during the genome sequencing of isolates from six species of parasites from the sub-family Leishmaniinae: Leishmania martiniquensis (Thailand), Leishmania orientalis (Thailand), Leishmania enriettii (Brazil), Leishmania sp. Ghana, Leishmania sp. Namibia and Porcisia hertigi (Panama). De novo assembly was performed using Nanopore long reads to construct chromosome backbone scaffolds. We then corrected erroneous base calling by mapping short Illumina paired-end reads onto the initial assembly. Data has been deposited at NCBI as follows: raw sequencing output in the Sequence Read Archive, finished genomes in GenBank, and ancillary data in BioSample and BioProject. Derived data such as quality scoring, SAM files, genome annotations and repeat sequence lists have been deposited in Lancaster University's electronic data archive with DOIs provided for each item. Our coding workflow has been deposited in GitHub and Zenodo repositories. This data constitutes a resource for the comparative genomics of parasites and for further applications in general and clinical parasitology.
AB - We provide the raw and processed data produced during the genome sequencing of isolates from six species of parasites from the sub-family Leishmaniinae: Leishmania martiniquensis (Thailand), Leishmania orientalis (Thailand), Leishmania enriettii (Brazil), Leishmania sp. Ghana, Leishmania sp. Namibia and Porcisia hertigi (Panama). De novo assembly was performed using Nanopore long reads to construct chromosome backbone scaffolds. We then corrected erroneous base calling by mapping short Illumina paired-end reads onto the initial assembly. Data has been deposited at NCBI as follows: raw sequencing output in the Sequence Read Archive, finished genomes in GenBank, and ancillary data in BioSample and BioProject. Derived data such as quality scoring, SAM files, genome annotations and repeat sequence lists have been deposited in Lancaster University's electronic data archive with DOIs provided for each item. Our coding workflow has been deposited in GitHub and Zenodo repositories. This data constitutes a resource for the comparative genomics of parasites and for further applications in general and clinical parasitology.
KW - Leishmaniinae
KW - Leishmania
KW - Porcisia
KW - genome sequencing
KW - genome assembly
KW - genome annotation
KW - Parasitology
KW - kinetoplastid
KW - Trypanosomatidae
U2 - 10.1038/s41597-021-01017-3
DO - 10.1038/s41597-021-01017-3
M3 - Journal article
C2 - 34489462
VL - 8
JO - Scientific Data
JF - Scientific Data
SN - 2052-4463
IS - 1
M1 - 234
ER -