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Circadian regulation of protein cargo in extracellular vesicles

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Circadian regulation of protein cargo in extracellular vesicles. / Yeung, C.-Y.C.; Dondelinger, F.; Schoof, E.M. et al.
In: Science Advances, Vol. 8, No. 14, eabc9061, 08.04.2022.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Yeung, C-YC, Dondelinger, F, Schoof, EM, Georg, B, Lu, Y, Zheng, Z, Zhang, J, Hannibal, J, Fahrenkrug, J & Kjaer, M 2022, 'Circadian regulation of protein cargo in extracellular vesicles', Science Advances, vol. 8, no. 14, eabc9061. https://doi.org/10.1126/sciadv.abc9061

APA

Yeung, C.-YC., Dondelinger, F., Schoof, E. M., Georg, B., Lu, Y., Zheng, Z., Zhang, J., Hannibal, J., Fahrenkrug, J., & Kjaer, M. (2022). Circadian regulation of protein cargo in extracellular vesicles. Science Advances, 8(14), Article eabc9061. https://doi.org/10.1126/sciadv.abc9061

Vancouver

Yeung CYC, Dondelinger F, Schoof EM, Georg B, Lu Y, Zheng Z et al. Circadian regulation of protein cargo in extracellular vesicles. Science Advances. 2022 Apr 8;8(14):eabc9061. doi: 10.1126/sciadv.abc9061

Author

Yeung, C.-Y.C. ; Dondelinger, F. ; Schoof, E.M. et al. / Circadian regulation of protein cargo in extracellular vesicles. In: Science Advances. 2022 ; Vol. 8, No. 14.

Bibtex

@article{3a2ee8e85b6a4615ae658059621383ab,
title = "Circadian regulation of protein cargo in extracellular vesicles",
abstract = "The circadian clock controls many aspects of physiology, but it remains undescribed whether extracellular vesicles (EVs), including exosomes, involved in cell-cell communications between tissues are regulated in a circadian pattern. We demonstrate a 24-hour rhythmic abundance of individual proteins in small EVs using liquid chromatography-mass spectrometry in circadian-synchronized tendon fibroblasts. Furthermore, the release of small EVs enriched in RNA binding proteins was temporally separated from those enriched in cytoskeletal and matrix proteins, which peaked during the end of the light phase. Last, we targeted the protein sorting mechanism in the exosome biogenesis pathway and established (by knockdown of circadian-regulated flotillin-1) that matrix metalloproteinase 14 abundance in tendon fibroblast small EVs is under flotillin-1 regulation. In conclusion, we have identified proteomic time signatures for small EVs released by tendon fibroblasts, which supports the view that the circadian clock regulates protein cargo in EVs involved in cell-cell cross-talk.",
author = "C.-Y.C. Yeung and F. Dondelinger and E.M. Schoof and B. Georg and Y. Lu and Z. Zheng and J. Zhang and J. Hannibal and J. Fahrenkrug and M. Kjaer",
year = "2022",
month = apr,
day = "8",
doi = "10.1126/sciadv.abc9061",
language = "English",
volume = "8",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "14",

}

RIS

TY - JOUR

T1 - Circadian regulation of protein cargo in extracellular vesicles

AU - Yeung, C.-Y.C.

AU - Dondelinger, F.

AU - Schoof, E.M.

AU - Georg, B.

AU - Lu, Y.

AU - Zheng, Z.

AU - Zhang, J.

AU - Hannibal, J.

AU - Fahrenkrug, J.

AU - Kjaer, M.

PY - 2022/4/8

Y1 - 2022/4/8

N2 - The circadian clock controls many aspects of physiology, but it remains undescribed whether extracellular vesicles (EVs), including exosomes, involved in cell-cell communications between tissues are regulated in a circadian pattern. We demonstrate a 24-hour rhythmic abundance of individual proteins in small EVs using liquid chromatography-mass spectrometry in circadian-synchronized tendon fibroblasts. Furthermore, the release of small EVs enriched in RNA binding proteins was temporally separated from those enriched in cytoskeletal and matrix proteins, which peaked during the end of the light phase. Last, we targeted the protein sorting mechanism in the exosome biogenesis pathway and established (by knockdown of circadian-regulated flotillin-1) that matrix metalloproteinase 14 abundance in tendon fibroblast small EVs is under flotillin-1 regulation. In conclusion, we have identified proteomic time signatures for small EVs released by tendon fibroblasts, which supports the view that the circadian clock regulates protein cargo in EVs involved in cell-cell cross-talk.

AB - The circadian clock controls many aspects of physiology, but it remains undescribed whether extracellular vesicles (EVs), including exosomes, involved in cell-cell communications between tissues are regulated in a circadian pattern. We demonstrate a 24-hour rhythmic abundance of individual proteins in small EVs using liquid chromatography-mass spectrometry in circadian-synchronized tendon fibroblasts. Furthermore, the release of small EVs enriched in RNA binding proteins was temporally separated from those enriched in cytoskeletal and matrix proteins, which peaked during the end of the light phase. Last, we targeted the protein sorting mechanism in the exosome biogenesis pathway and established (by knockdown of circadian-regulated flotillin-1) that matrix metalloproteinase 14 abundance in tendon fibroblast small EVs is under flotillin-1 regulation. In conclusion, we have identified proteomic time signatures for small EVs released by tendon fibroblasts, which supports the view that the circadian clock regulates protein cargo in EVs involved in cell-cell cross-talk.

U2 - 10.1126/sciadv.abc9061

DO - 10.1126/sciadv.abc9061

M3 - Journal article

VL - 8

JO - Science Advances

JF - Science Advances

SN - 2375-2548

IS - 14

M1 - eabc9061

ER -