Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue. / Gaur, Nishtha; Szili, Endre J.; Oh, Jun-Seok et al.
In: Applied Physics Letters, Vol. 107, No. 10, 103703, 07.09.2015.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Gaur, N, Szili, EJ, Oh, J-S, Hong, S-H, Michelmore, A, Graves, DB, Hatta, A & Short, RD 2015, 'Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue', Applied Physics Letters, vol. 107, no. 10, 103703. https://doi.org/10.1063/1.4930874

APA

Gaur, N., Szili, E. J., Oh, J-S., Hong, S-H., Michelmore, A., Graves, D. B., Hatta, A., & Short, R. D. (2015). Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue. Applied Physics Letters, 107(10), Article 103703. https://doi.org/10.1063/1.4930874

Vancouver

Gaur N, Szili EJ, Oh J-S, Hong S-H, Michelmore A, Graves DB et al. Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue. Applied Physics Letters. 2015 Sept 7;107(10):103703. doi: 10.1063/1.4930874

Author

Gaur, Nishtha ; Szili, Endre J. ; Oh, Jun-Seok et al. / Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue. In: Applied Physics Letters. 2015 ; Vol. 107, No. 10.

Bibtex

@article{6d1e559e3a43411ea059bee680623532,

title = "Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue",

abstract = "The influence of protein and molecular, ground state oxygen (O-2) on the plasma generation, and transport of reactive oxygen and nitrogen species (RONS) in tissue are investigated. A tissue target, comprising a 1mm thick gelatin film (a surrogate for real tissue), is placed on top of a 96-well plate; each well is filled with phosphate buffered saline (PBS, pH 7.4) containing one fluorescent or colorimetric reporter that is specific for one of three RONS (i.e., H2O2, NO2-, or OH center dot) or a broad spectrum reactive oxygen species reporter (2,7-dichlorodihydrofluorescein). A helium cold atmospheric plasma (CAP) jet contacts the top of the gelatin surface, and the concentrations of RONS generated in PBS are measured on a microplate reader. The data show that H2O2, NO2-, or OH center dot are generated in PBS underneath the target. Independently, measurements are made of the O-2 concentration in the PBS with and without the gelatin target. Adding bovine serum albumin protein to the PBS or gelatin shows that protein either raises or inhibits RONS depending upon the O-2 concentration. Our results are discussed in the context of plasma-soft tissue interactions that are important in the development of CAP technology for medicine, biology, and food manufacturing. (C) 2015 AIP Publishing LLC.",

keywords = "RANDOMIZED CONTROLLED-TRIAL, ATMOSPHERIC ARGON PLASMA, PRESSURE COLD-PLASMA, DISCHARGE PLASMA, CANCER-THERAPY, CHRONIC WOUNDS, SKIN CELLS, DNA-DAMAGE, GAS-PLASMA, DECONTAMINATION",

author = "Nishtha Gaur and Szili, {Endre J.} and Jun-Seok Oh and Sung-Ha Hong and Andrew Michelmore and Graves, {David B.} and Akimitsu Hatta and Short, {Robert D.}",

year = "2015",

month = sep,

day = "7",

doi = "10.1063/1.4930874",

language = "English",

volume = "107",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Inc.",

number = "10",

}

RIS

TY - JOUR

T1 - Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue

AU - Gaur, Nishtha

AU - Szili, Endre J.

AU - Oh, Jun-Seok

AU - Hong, Sung-Ha

AU - Michelmore, Andrew

AU - Graves, David B.

AU - Hatta, Akimitsu

AU - Short, Robert D.

PY - 2015/9/7

Y1 - 2015/9/7

N2 - The influence of protein and molecular, ground state oxygen (O-2) on the plasma generation, and transport of reactive oxygen and nitrogen species (RONS) in tissue are investigated. A tissue target, comprising a 1mm thick gelatin film (a surrogate for real tissue), is placed on top of a 96-well plate; each well is filled with phosphate buffered saline (PBS, pH 7.4) containing one fluorescent or colorimetric reporter that is specific for one of three RONS (i.e., H2O2, NO2-, or OH center dot) or a broad spectrum reactive oxygen species reporter (2,7-dichlorodihydrofluorescein). A helium cold atmospheric plasma (CAP) jet contacts the top of the gelatin surface, and the concentrations of RONS generated in PBS are measured on a microplate reader. The data show that H2O2, NO2-, or OH center dot are generated in PBS underneath the target. Independently, measurements are made of the O-2 concentration in the PBS with and without the gelatin target. Adding bovine serum albumin protein to the PBS or gelatin shows that protein either raises or inhibits RONS depending upon the O-2 concentration. Our results are discussed in the context of plasma-soft tissue interactions that are important in the development of CAP technology for medicine, biology, and food manufacturing. (C) 2015 AIP Publishing LLC.

AB - The influence of protein and molecular, ground state oxygen (O-2) on the plasma generation, and transport of reactive oxygen and nitrogen species (RONS) in tissue are investigated. A tissue target, comprising a 1mm thick gelatin film (a surrogate for real tissue), is placed on top of a 96-well plate; each well is filled with phosphate buffered saline (PBS, pH 7.4) containing one fluorescent or colorimetric reporter that is specific for one of three RONS (i.e., H2O2, NO2-, or OH center dot) or a broad spectrum reactive oxygen species reporter (2,7-dichlorodihydrofluorescein). A helium cold atmospheric plasma (CAP) jet contacts the top of the gelatin surface, and the concentrations of RONS generated in PBS are measured on a microplate reader. The data show that H2O2, NO2-, or OH center dot are generated in PBS underneath the target. Independently, measurements are made of the O-2 concentration in the PBS with and without the gelatin target. Adding bovine serum albumin protein to the PBS or gelatin shows that protein either raises or inhibits RONS depending upon the O-2 concentration. Our results are discussed in the context of plasma-soft tissue interactions that are important in the development of CAP technology for medicine, biology, and food manufacturing. (C) 2015 AIP Publishing LLC.

KW - RANDOMIZED CONTROLLED-TRIAL

KW - ATMOSPHERIC ARGON PLASMA

KW - PRESSURE COLD-PLASMA

KW - DISCHARGE PLASMA

KW - CANCER-THERAPY

KW - CHRONIC WOUNDS

KW - SKIN CELLS

KW - DNA-DAMAGE

KW - GAS-PLASMA

KW - DECONTAMINATION

U2 - 10.1063/1.4930874

DO - 10.1063/1.4930874

M3 - Journal article

VL - 107

JO - Applied Physics Letters

JF - Applied Physics Letters

SN - 0003-6951

IS - 10

M1 - 103703

ER -

Research

Associated organisational unit

Links

Text available via DOI:

Keywords