Home > Research > Publications & Outputs > Comparative proteomics of Pinus – Fusarium circ...

Links

Text available via DOI:

View graph of relations

Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance. / Amaral, Joana; Lamelas, Laura; Valledor, Luis; Castillejo, María Ángeles; Alves, Artur; Pinto, Glória.

In: Physiologia Plantarum, Vol. 173, No. 4, 31.12.2021, p. 2142-2154.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Amaral, J, Lamelas, L, Valledor, L, Castillejo, MÁ, Alves, A & Pinto, G 2021, 'Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance', Physiologia Plantarum, vol. 173, no. 4, pp. 2142-2154. https://doi.org/10.1111/ppl.13563

APA

Amaral, J., Lamelas, L., Valledor, L., Castillejo, M. Á., Alves, A., & Pinto, G. (2021). Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance. Physiologia Plantarum, 173(4), 2142-2154. https://doi.org/10.1111/ppl.13563

Vancouver

Amaral J, Lamelas L, Valledor L, Castillejo MÁ, Alves A, Pinto G. Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance. Physiologia Plantarum. 2021 Dec 31;173(4):2142-2154. https://doi.org/10.1111/ppl.13563

Author

Amaral, Joana ; Lamelas, Laura ; Valledor, Luis ; Castillejo, María Ángeles ; Alves, Artur ; Pinto, Glória. / Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance. In: Physiologia Plantarum. 2021 ; Vol. 173, No. 4. pp. 2142-2154.

Bibtex

@article{eb4b7e293e424cfc9115ff33b0dee862,
title = "Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance",
abstract = "Fusarium circinatum, causing pine pitch canker (PPC), affects conifers productivity and health worldwide. Selection and breeding for resistance arises as the most promising approach to fight PPC. Therefore, it is crucial to explore the response of hosts with varying levels of susceptibility to PPC to unveil the genes/pathways behind these phenotypes. We evaluated the dynamics of the needle proteome of a susceptible (Pinus radiata) and a relatively resistant (Pinus pinea) species upon F. circinatum inoculation by GeLC-MS/MS. Integration with physiological data and validation of key genes by qPCR allowed to identify core pathways regulating these contrasting responses. In P. radiata, the pathogen may target both the secondary metabolism to negatively regulate immune response and chloroplast redox proteins to increase energy-producing pathways for amino acid production in its favour. In contrast, chloroplast redox regulation may assure redox homeostasis in P. pinea, as well as nonenzymatic antioxidants. The presence of membrane trafficking-related proteins exclusively in P. pinea likely explains its defence response against F. circinatum. A crosstalk between abscisic acid and epigenetic regulation of gene expression is also proposed in PPC response. These results are useful to support breeding programs aiming to achieve PPC resistance.",
author = "Joana Amaral and Laura Lamelas and Luis Valledor and Castillejo, {Mar{\'i}a {\'A}ngeles} and Artur Alves and Gl{\'o}ria Pinto",
year = "2021",
month = dec,
day = "31",
doi = "10.1111/ppl.13563",
language = "English",
volume = "173",
pages = "2142--2154",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Blackwell-Wiley",
number = "4",

}

RIS

TY - JOUR

T1 - Comparative proteomics of Pinus – Fusarium circinatum interactions reveal metabolic clues to biotic stress resistance

AU - Amaral, Joana

AU - Lamelas, Laura

AU - Valledor, Luis

AU - Castillejo, María Ángeles

AU - Alves, Artur

AU - Pinto, Glória

PY - 2021/12/31

Y1 - 2021/12/31

N2 - Fusarium circinatum, causing pine pitch canker (PPC), affects conifers productivity and health worldwide. Selection and breeding for resistance arises as the most promising approach to fight PPC. Therefore, it is crucial to explore the response of hosts with varying levels of susceptibility to PPC to unveil the genes/pathways behind these phenotypes. We evaluated the dynamics of the needle proteome of a susceptible (Pinus radiata) and a relatively resistant (Pinus pinea) species upon F. circinatum inoculation by GeLC-MS/MS. Integration with physiological data and validation of key genes by qPCR allowed to identify core pathways regulating these contrasting responses. In P. radiata, the pathogen may target both the secondary metabolism to negatively regulate immune response and chloroplast redox proteins to increase energy-producing pathways for amino acid production in its favour. In contrast, chloroplast redox regulation may assure redox homeostasis in P. pinea, as well as nonenzymatic antioxidants. The presence of membrane trafficking-related proteins exclusively in P. pinea likely explains its defence response against F. circinatum. A crosstalk between abscisic acid and epigenetic regulation of gene expression is also proposed in PPC response. These results are useful to support breeding programs aiming to achieve PPC resistance.

AB - Fusarium circinatum, causing pine pitch canker (PPC), affects conifers productivity and health worldwide. Selection and breeding for resistance arises as the most promising approach to fight PPC. Therefore, it is crucial to explore the response of hosts with varying levels of susceptibility to PPC to unveil the genes/pathways behind these phenotypes. We evaluated the dynamics of the needle proteome of a susceptible (Pinus radiata) and a relatively resistant (Pinus pinea) species upon F. circinatum inoculation by GeLC-MS/MS. Integration with physiological data and validation of key genes by qPCR allowed to identify core pathways regulating these contrasting responses. In P. radiata, the pathogen may target both the secondary metabolism to negatively regulate immune response and chloroplast redox proteins to increase energy-producing pathways for amino acid production in its favour. In contrast, chloroplast redox regulation may assure redox homeostasis in P. pinea, as well as nonenzymatic antioxidants. The presence of membrane trafficking-related proteins exclusively in P. pinea likely explains its defence response against F. circinatum. A crosstalk between abscisic acid and epigenetic regulation of gene expression is also proposed in PPC response. These results are useful to support breeding programs aiming to achieve PPC resistance.

U2 - 10.1111/ppl.13563

DO - 10.1111/ppl.13563

M3 - Journal article

VL - 173

SP - 2142

EP - 2154

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 4

ER -